| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Embryonic stem cells are the stem cells that are established from an early stage of a developing embryo. ES cells are the origin of cell types of several hundreds that compose our bodies, and they are known to hold properties that are unique in various aspects of cell division cycle. During this research period, we found that replication machinery progress slowly at all stage of DNA replication (S) phase, and found that this is due to reduced amount of dNTPs. Despite the slow replication-machinery, ES cells complete DNA synthesis within similar amount of time compared to MEFs. Our results show that this is due to denser distribution of active DNA replication machinery along the genome.
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