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Development of the lateral line system through local tissue-tissue interactions in the zebrafish head

Research Project

Project/Area Number 16K07386
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionKitasato University

Principal Investigator

Wada Hironori  北里大学, 一般教育部, 准教授 (70322708)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsゼブラフィッシュ / 側線 / 神経堤細胞 / プラコード / Rspo2 / Wnt signaling / 側線神経 / 感丘 / 有毛細胞 / 軟骨 / 硬骨 / 器官形成 / 組織間相互作用
Outline of Final Research Achievements

The distribution of sensory organs is important for detecting environmental signals efficiently. The mechanosensory receptors of the lateral line system, neuromasts, are stereotypically distributed over the head and body surface of fish.We investigated development of the anterior lateral line (ALL) system in zebrafish head. The ALL neuromasts formed in the predetermined positions through proliferation and differentiation of 1) non-migratory lateral line primordia, 2) migratory primordia, 3) interneuromast cells connecting pre-existing neuromasts, and 4) budding primordia. We demonstrated that R-spondin2 (Rspo2), an activator of Wnt/β-catenin signaling, is required for the development of a particular set of neuromasts associated with hyomandibular cartilage. Further genetic analyses suggested that Rspo2, which emanates from the hyoid mesenchyme, acts on the adjacent neuromast progenitor cells to stimulate their proliferation through activating Wnt/β-catenin signaling.

Academic Significance and Societal Importance of the Research Achievements

動物は、体の決まった場所に感覚器をつくることにより、様々な環境に適応してきた。しかし、感覚器を決まった場所につくる仕組みはよく分かっていない。この研究では、魚類の側線器官(水の流れを感じる器官)の発生過程において、頭部の間葉組織(結合組織)から分泌されるタンパク質が、感覚器の細胞増殖を制御していることを示した。側線器官の前駆細胞は、増殖や移動を伴って、頭部に複雑なネットワークを作っている。特定の場所から分泌される増殖因子が働くことで、その場所に感覚器が形成される。このような、決まった場所でおきる組織どうしの相互作用が、感覚器の多様なパターンを生み出している。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2020 2019 2018 2017 2016 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Open Access: 3 results,  Peer Reviewed: 4 results) Presentation (8 results) (of which Invited: 2 results) Book (1 results) Remarks (1 results)

  • [Journal Article] ゼブラフィッシュ側線器官のパターン形成メカニズム2020

    • Author(s)
      和田浩則
    • Journal Title

      Comparative Endocrinology

      Volume: 46 Issue: 169 Pages: 16-18

    • DOI

      10.5983/nl2008jsce.46.16

    • NAID

      130007806047

    • ISSN
      1882-6636, 1882-6644
    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Journal Article] Regulation of Scale Morphogenesis through Wnt/PCP Signaling in Zebrafish2019

    • Author(s)
      Iwasaki M, Wada H
    • Journal Title

      CYTOLOGIA

      Volume: 84 Issue: 4 Pages: 293-294

    • DOI

      10.1508/cytologia.84.293

    • NAID

      130007769551

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2019-12-25
    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Journal Article] Epidermal regulation of bone morphogenesis through the development and regeneration of osteoblasts in the zebrafish scale.2018

    • Author(s)
      Iwasaki M, Kuroda J, Kawakami K, Wada H.
    • Journal Title

      Dev Biol.

      Volume: 437 Issue: 2 Pages: 105-119

    • DOI

      10.1016/j.ydbio.2018.03.005

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish.2018

    • Author(s)
      Lal P, Tanabe H, Suster ML, Ailani D, Kotani Y, Muto A, Itoh M, Iwasaki M, Wada H, Yaksi E, Kawakami K.
    • Journal Title

      BMC Biol.

      Volume: 16 Issue: 1 Pages: 45-45

    • DOI

      10.1186/s12915-018-0502-y

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gal4 Driver Transgenic Zebrafish: Powerful Tools to StudyDevelopmental Biology, Organogenesis, and Neuroscience2016

    • Author(s)
      Kawakami K, Asakawa K, Hibi M, Itoh M, Muto A, Wada H.
    • Journal Title

      Adv Genet.

      Volume: 95 Pages: 65-87

    • DOI

      10.1016/bs.adgen.2016.04.002

    • ISBN
      9780128048009
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tol2-mediated transgenesis, gene trapping, enhancer trapping, and Gal4-UAS system2016

    • Author(s)
      Kawakami K, Asakawa K, Muto A, Wada H.
    • Journal Title

      Methods Cell Biol.

      Volume: 135 Pages: 19-37

    • DOI

      10.1016/bs.mcb.2016.01.011

    • ISBN
      9780128034743
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Jaw cartilage regulates proliferation of the lateral line sensory organs through Wnt signaling in zebrafish2019

    • Author(s)
      Iwasaki M, Yokoi H, Suzuki T, Kawakami K, Wada H
    • Organizer
      The 25th Japanese medaka and zebrafish meeting
    • Related Report
      2019 Annual Research Report
  • [Presentation] ゼブラフィッシュ鱗の発生と再生2019

    • Author(s)
      和田浩則
    • Organizer
      第二回 再生学異分野融合研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 魚類側線系の多様性を生み出す発生システム2019

    • Author(s)
      和田浩則
    • Organizer
      魚類分類研究会
    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Invited
  • [Presentation] Epidermal regulation of bone patterning through the development and regeneration of osteoblasts in the zebrafish scale2018

    • Author(s)
      Iwasaki M, Kuroda J, Kawakami K, Wada H.
    • Organizer
      Tokyo 2018 Cell and Developmental Biology Meeting
    • Related Report
      2018 Research-status Report
  • [Presentation] Epidermal patterning regulates directional bone growth during scale development in zebrafish2017

    • Author(s)
      Iwasaki M, Kawakami K, Wada H.
    • Organizer
      The 23rd Japanese Medaka and Zebrafish Meeting
    • Related Report
      2017 Research-status Report
  • [Presentation] ゼブラフィッシュ頭部側線神経のパターン形成2017

    • Author(s)
      和田浩則、川上浩一、岩崎美樹
    • Organizer
      第7回 Tokyo Vertebrate Morphology Meeting
    • Related Report
      2017 Research-status Report
  • [Presentation] ゼブラフィッシュにおいて表皮のパターンが鱗の形態形成を制御する2017

    • Author(s)
      岩崎 美樹、川上 浩一、和田 浩則
    • Organizer
      第40回 日本分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] ゼブラフィッシュの鱗における皮骨形成過程の解析2016

    • Author(s)
      岩崎美樹・川上浩一・和田浩則
    • Organizer
      第6回 Tokyo Vertebrate Morphology Meeting
    • Place of Presentation
      東京慈恵会医科大学(東京都・港区)
    • Year and Date
      2016-08-06
    • Related Report
      2016 Research-status Report
  • [Book] 生き物と音の事典2019

    • Author(s)
      和田浩則(分担)生物音響学会編
    • Total Pages
      464
    • Publisher
      朝倉書店
    • ISBN
      9784254171679
    • Related Report
      2019 Annual Research Report
  • [Remarks] ようこそ和田のホームページへ

    • URL

      http://w01.tp1.jp/~a680124941/Wadas_home_page/homu.html

    • Related Report
      2019 Annual Research Report 2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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