Development of the lateral line system through local tissue-tissue interactions in the zebrafish head

• Project/Area Number: 16K07386
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kitasato University
• Principal Investigator: Wada Hironori 北里大学, 一般教育部, 准教授 (70322708)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ゼブラフィッシュ / 側線 / 神経堤細胞 / プラコード / Rspo2 / Wnt signaling / 側線神経 / 感丘 / 有毛細胞 / 軟骨 / 硬骨 / 器官形成 / 組織間相互作用

Outline of Final Research Achievements

The distribution of sensory organs is important for detecting environmental signals efficiently. The mechanosensory receptors of the lateral line system, neuromasts, are stereotypically distributed over the head and body surface of fish.We investigated development of the anterior lateral line (ALL) system in zebrafish head. The ALL neuromasts formed in the predetermined positions through proliferation and differentiation of 1) non-migratory lateral line primordia, 2) migratory primordia, 3) interneuromast cells connecting pre-existing neuromasts, and 4) budding primordia. We demonstrated that R-spondin2 (Rspo2), an activator of Wnt/β-catenin signaling, is required for the development of a particular set of neuromasts associated with hyomandibular cartilage. Further genetic analyses suggested that Rspo2, which emanates from the hyoid mesenchyme, acts on the adjacent neuromast progenitor cells to stimulate their proliferation through activating Wnt/β-catenin signaling.

Academic Significance and Societal Importance of the Research Achievements

動物は、体の決まった場所に感覚器をつくることにより、様々な環境に適応してきた。しかし、感覚器を決まった場所につくる仕組みはよく分かっていない。この研究では、魚類の側線器官（水の流れを感じる器官）の発生過程において、頭部の間葉組織（結合組織）から分泌されるタンパク質が、感覚器の細胞増殖を制御していることを示した。側線器官の前駆細胞は、増殖や移動を伴って、頭部に複雑なネットワークを作っている。特定の場所から分泌される増殖因子が働くことで、その場所に感覚器が形成される。このような、決まった場所でおきる組織どうしの相互作用が、感覚器の多様なパターンを生み出している。

Research Products

• [Journal Article] ゼブラフィッシュ側線器官のパターン形成メカニズム (2020)
  • Author(s): 和田浩則
  • Journal Title: Comparative Endocrinology Volume: 46 Issue: 169 Pages: 16-18
  • DOI: 10.5983/nl2008jsce.46.16
  • NAID: 130007806047
  • ISSN: 1882-6636, 1882-6644
  • Open Access
• [Journal Article] Regulation of Scale Morphogenesis through Wnt/PCP Signaling in Zebrafish (2019)
  • Author(s): Iwasaki M, Wada H
  • Journal Title: CYTOLOGIA Volume: 84 Issue: 4 Pages: 293-294
  • DOI: 10.1508/cytologia.84.293
  • NAID: 130007769551
  • ISSN: 0011-4545, 1348-7019
  • Year and Date: 2019-12-25
  • Open Access
• [Journal Article] Epidermal regulation of bone morphogenesis through the development and regeneration of osteoblasts in the zebrafish scale. (2018)
  • Author(s): Iwasaki M, Kuroda J, Kawakami K, Wada H.
  • Journal Title: Dev Biol. Volume: 437 Issue: 2 Pages: 105-119
  • DOI: 10.1016/j.ydbio.2018.03.005
  • Peer Reviewed
• [Journal Article] Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish. (2018)
  • Author(s): Lal P, Tanabe H, Suster ML, Ailani D, Kotani Y, Muto A, Itoh M, Iwasaki M, Wada H, Yaksi E, Kawakami K.
  • Journal Title: BMC Biol. Volume: 16 Issue: 1 Pages: 45-45
  • DOI: 10.1186/s12915-018-0502-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gal4 Driver Transgenic Zebrafish: Powerful Tools to StudyDevelopmental Biology, Organogenesis, and Neuroscience (2016)
  • Author(s): Kawakami K, Asakawa K, Hibi M, Itoh M, Muto A, Wada H.
  • Journal Title: Adv Genet. Volume: 95 Pages: 65-87
  • DOI: 10.1016/bs.adgen.2016.04.002
  • Peer Reviewed
• [Journal Article] Tol2-mediated transgenesis, gene trapping, enhancer trapping, and Gal4-UAS system (2016)
  • Author(s): Kawakami K, Asakawa K, Muto A, Wada H.
  • Journal Title: Methods Cell Biol. Volume: 135 Pages: 19-37
  • DOI: 10.1016/bs.mcb.2016.01.011
  • ISBN: 9780128034743
  • Peer Reviewed
• [Presentation] Jaw cartilage regulates proliferation of the lateral line sensory organs through Wnt signaling in zebrafish (2019)
  • Author(s): Iwasaki M, Yokoi H, Suzuki T, Kawakami K, Wada H
  • Organizer: The 25th Japanese medaka and zebrafish meeting
• [Presentation] ゼブラフィッシュ鱗の発生と再生 (2019)
  • Author(s): 和田浩則
  • Organizer: 第二回 再生学異分野融合研究会
  • Invited
• [Presentation] 魚類側線系の多様性を生み出す発生システム (2019)
  • Author(s): 和田浩則
  • Organizer: 魚類分類研究会
  • Invited
• [Presentation] Epidermal regulation of bone patterning through the development and regeneration of osteoblasts in the zebrafish scale (2018)
  • Author(s): Iwasaki M, Kuroda J, Kawakami K, Wada H.
  • Organizer: Tokyo 2018 Cell and Developmental Biology Meeting
• [Presentation] Epidermal patterning regulates directional bone growth during scale development in zebrafish (2017)
  • Author(s): Iwasaki M, Kawakami K, Wada H.
  • Organizer: The 23rd Japanese Medaka and Zebrafish Meeting
• [Presentation] ゼブラフィッシュ頭部側線神経のパターン形成 (2017)
  • Author(s): 和田浩則、川上浩一、岩崎美樹
  • Organizer: 第７回 Tokyo Vertebrate Morphology Meeting
• [Presentation] ゼブラフィッシュにおいて表皮のパターンが鱗の形態形成を制御する (2017)
  • Author(s): 岩崎 美樹、川上 浩一、和田 浩則
  • Organizer: 第４０回 日本分子生物学会
• [Presentation] ゼブラフィッシュの鱗における皮骨形成過程の解析 (2016)
  • Author(s): 岩崎美樹・川上浩一・和田浩則
  • Organizer: 第６回 Tokyo Vertebrate Morphology Meeting
  • Place of Presentation: 東京慈恵会医科大学（東京都・港区）
  • Year and Date: 2016-08-06
• [Book] 生き物と音の事典 (2019)
  • Author(s): 和田浩則（分担）生物音響学会編
  • Total Pages: 464
  • Publisher: 朝倉書店
  • ISBN: 9784254171679
• [Remarks] ようこそ和田のホームページへ
  • URL: http://w01.tp1.jp/~a680124941/Wadas_home_page/homu.html