New roles and mechanisms of translational regulation mediated by upstream open reading frame-encoded peptides
| Project/Area Number |
16K07387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Research Collaborator |
HAYASHI noriya
UMEHARA shun-ichi
SASAKI shun
SASAHARA daiki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | uORF / リボソーム / 翻訳制御 / 翻訳アレスト / 新生ペプチド / 被子植物 |
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| Outline of Final Research Achievements |
Upstream open reading frames (uORFs) are small ORFs located in the 5′ untranslated regions (5′-UTRs) of many eukaryotic mRNAs. Some uORFs encode regulatory peptides that repress translation of the downstream main ORF. Most of the previously characterized uORFs encoding regulatory peptides play a role in feedback regulation in response to a metabolite. To identify new physiological roles of uORF-encoded regulatory peptides, we searched for uORFs encoding regulatory peptides among angiosperm uORFs with evolutionarily conserved amino acid sequences, and identified nine novel regulatory uORFs. We investigated physiological roles and mechanisms of the translational regulation mediated by these uORFs. We identified a uORF encoding a peptide that causes ribosome stalling in response to magnesium and thereby represses main ORF translation under high magnesium conditions. In addition, we identified a uORF involved in translational regulation in response to nucleolar stress.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、マグネシウムに応答してリボソームの停滞を引き起こし、それにより主要ORFの翻訳を抑制するuORFペプチドを見出した。これまでに代謝産物に応答してリボソームを停滞させるuORFペプチドは知られていたが、無機イオンに応答してリボソームを停滞させるuORFペプチドとしては、これが初めての発見である。また、核小体ストレスに応答して誘導される選択的スプライシングにより、uORFが除去されたmRNAが生産され、それによって主要ORFの翻訳が促進されるという、ストレスに応答した新たな翻訳制御機構を見出した。
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Report
(4 results)
Research Products
(13 results)
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[Presentation] Nucleolar stress promotes expression of Arabidopsis ANAC082, a nucleolar stress response mediator, by inducing alternative splicing that removes an inhibitory upstream open reading frame2019
Author(s)
Shun Sasaki, Rin Kudo, Daiki Sasahara, Hiro Takahashi, Shun Watanabe, Iwai Ohbayashi, Munataka Sugiyama, Satoshi Naito, Hitoshi Onouchi
Organizer
Post-transcriptional Gene Regulation in Plants (PGRP) 2019
Related Report
Int'l Joint Research
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[Presentation] A Critical Role of the NAC transcription Factor ANAC082 in Ribosomal Stress Signaling of Plant Cells2018
Author(s)
Iwai Ohbayashi, Shun Sasaki, Chung-Yi Lin, Naoki Shinohara, Yoko Matsumura, Yasunori Machida, Gorou Horiguchi, Hirokazu Tsukaya, Masahiko Furutani, Hitoshi Onouchi, and Munetaka Sugiyama
Organizer
第59回日本植物生理学会年会
Related Report
Invited
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