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A study on molecualr mechanisms of lipid signaling and G protein function in plant endoplasmic reticulum stress response

Research Project

Project/Area Number 16K07388
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionMuroran Institute of Technology

Principal Investigator

Kanehara Kazue  室蘭工業大学, 大学院工学研究科, その他(客員准教授) (30587746)

Co-Investigator(Kenkyū-buntansha) 岩佐 達郎  室蘭工業大学, 大学院工学研究科, 名誉教授 (00133926)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords小胞体ストレス / Gタンパク質 / 脂質シグナリング / シロイヌナズナ / 小胞体ストレス応答 / 三量体Gタンパク複合体 / Gβサブユニット / 小胞体 / シグナル伝達 / オルガネラ
Outline of Final Research Achievements

Living cells always need to maintain cellular homeostasis while they continuously suffered from various stresses. Recent studies have revealed molecular mechanisms of stress responses at the endoplasmic reticulum in higher plants that are exposed to high temperature or salinity.
In our research project, we successfully established Arabidopsis transgenic lines that allow us to visualize the endoplasmic stress response in vivo. In addition, we found that one of fatty acid desaturases is important in endoplasmic reticulum stress tolerance. We published two original articles in international journals that report these results.

Academic Significance and Societal Importance of the Research Achievements

タンパク質の品質管理に焦点をあてた小胞体ストレス応答の分子メカニズムは、主に動物細胞を用いた研究からその全容が明らかになりつつあるが、高等植物における知見は未だ限られてる。本研究で得られた成果は、植物の小胞体ストレス応答における理解を通じて、高ストレス耐性植物の分子育種に新たな方法を提示し植物バイオマスの増産に貢献する方法論を拓く可能性があるため、生物学的意義だけでなく、農産業的にも極めて意義深い。

Report

(3 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Journal Article] Endoplasmic reticulum stress response in Arabidopsis roots2017

    • Author(s)
      Yueh Cho and Kazue Kanehara
    • Journal Title

      Frontiers in Plant Science

      Volume: 8 Pages: 1-10

    • DOI

      10.3389/fpls.2017.00144

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Endoplasmic reticulum stress response in Arabidopsis roots2017

    • Author(s)
      Yueh Cho、Kazue kanehara
    • Organizer
      EMBO Workshop: Intercellular communication in development and disease
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] The link between endoplasmic reticulum stress response and phosphoinositide signaling in higher plants2017

    • Author(s)
      Kazue Kanehara
    • Organizer
      IoC-IPMB-ITbM Jpint Symposium on New Frontiers by Fusing Chemistry and Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Arabidopsis AGB1 in endoplasmic reticulum stress response2017

    • Author(s)
      Yueh Cho, Tatsuo Iwasa, Kazue Kanehara
    • Organizer
      The 7th Asian Symposium on Plant Lipid
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Phosphoinositide signaling involved in the endoplasmic reticulum stress response in higher plants2016

    • Author(s)
      Kazue kanehara, Chao-Yuan Yu, Markus R Wenk, and Tatsuo Iwasa
    • Organizer
      EMBO conference: Structure and function of the endoplasmic reticulum
    • Place of Presentation
      Hotel PGA Catalunya Business & Convention Centre, Girona, Spain
    • Year and Date
      2016-10-24
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Arabidopsis phosphoinositide-specific phospholipase C2, AtPLC2, involved in the endoplasmic reticulum stress tolerance2016

    • Author(s)
      Yueh Cho,Tatsuo Iwasa, Markus R Wenk, and Kazue Kanehara
    • Organizer
      57th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Convention Centre LE MAJESTIC, Chamonix, France
    • Year and Date
      2016-09-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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