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The mechanical bias induced by cell wall dynamics plays a key role in the regulation of cell morphogenesis in plants

Research Project

Project/Area Number 16K07389
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionTohoku University

Principal Investigator

YOKOYAMA Ryusuke  東北大学, 生命科学研究科, 講師 (90302083)

Research Collaborator NISHITANI kazuhiko  東北大学, 大学院生命科学研究科, 教授 (60164555)
KUROHA takeshi  東北大学, 大学院生命科学研究科, 助教 (50415155)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords植物細胞壁 / プロトプラスト / セルロース / キシログルカン / ネットワーク構造 / イメージング / マトリックス構造 / 細胞壁 / 細胞形成 / 物理的バイアス
Outline of Final Research Achievements

Cell wall dynamics have been shown to play a key role in the regulation of cell expansion in plants. However, cell wall dynamics results not only in cell wall relaxation, but also in a highly biased pattern of cell wall mechanics. The mechanical bias due to the dynamic structure of cell wall may determine cell polarity and growth patterns, and trigger cell differentiation. In this study, we developed a quantitative confocal imaging method for general use in the study of cell wall dynamics in protoplasts derived from Arabidopsis leaf mesophyll cells. Confocal imaging of regenerating cell walls in protoplasts stained with Calcofluor White M2R allowed us to visualize the cellulose network, the basic framework that functions as the mechanical underpinning of the cell wall. Using the image analysis techniques, we successfully characterized dynamics of the cellulose network, which provides a mechanical bias for functional expression of cell wall in cell morphogenesis.

Academic Significance and Societal Importance of the Research Achievements

植物の形態形成は,発生プログラムや環境シグナルによる統御を受け,最終的には細胞壁の強靱性と伸展性という2つの物理的性質の相互変換によって制御される細胞形成に依存している。近年,植物においても,細胞内情報伝達や遺伝子発現の制御機構の研究が飛躍的な進歩を遂げる一方で,細胞壁による細胞形成の分子機構の解明は大きく遅れている。本研究で細胞形成に寄与する細胞壁の機能的構造のダイナミクスを明らかにできたことは,植物の形態形成メカニズムの総合的な理解に繋がるものであり,この研究成果は,学術的に意義があるだけではなく,農業における品種改良などでも大きな社会貢献に繋がるものと考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (38 results)

All 2019 2018 2017 2016 Other

All Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (28 results) (of which Int'l Joint Research: 9 results) Remarks (2 results)

  • [Journal Article] 植物細胞壁の機能的構造とダイナミクス2019

    • Author(s)
      横山隆亮
    • Journal Title

      アグリバイオ

      Volume: 3 Pages: 43-47

    • NAID

      40021843572

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Ethylene-gibberellin signaling underlies adaptation of rice to periodic flooding2018

    • Author(s)
      T Kuroha, K Nagai, R Gamuyao, D R Wang, T Furuta, M Nakamori, T Kitaoka, K Adachi, A Minami, Y Mori, K Mashiguchi, Y Seto, S Yamaguchi, M Kojima, H Sakakibara, J Wu, K Ebana, N Mitsuda, M Ohme-Takagi, S Yanagisawa, M Yamasaki, R Yokoyama, K Nishitani, T Mochizuki, G Tamiya, S R McCouch, and M Ashikari
    • Journal Title

      Science

      Volume: 361 Issue: 6398 Pages: 181-186

    • DOI

      10.1126/science.aat1577

    • NAID

      120006529624

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy.2018

    • Author(s)
      Yamaguchi, N., Huang, J., Tatsumi, Y., Abe, M., Sugano, S. S., Kojima, M., Takebayashi, Y., Kiba, T., Yokoyama, R., Nishitani, K., Sakakibara, H., and Ito, T.
    • Journal Title

      Nature Commun.

      Volume: 9 Issue: 1 Pages: 5290-5290

    • DOI

      10.1038/s41467-018-07763-0

    • NAID

      120006549567

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Arabinogalactan proteins accumulate in the cell walls of searching hyphae of the stem parasitic plants, Cuscuta campestris and Cuscuta japonica.2017

    • Author(s)
      Hozumi A, Bera S, Fujiwara D, Obayashi T, Yokoyama R, Nishitani K, Aoki K
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 11 Pages: 1868-1877

    • DOI

      10.1093/pcp/pcx121

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.2017

    • Author(s)
      Bowman J, Kouchi T,..(106名)..Yokoyama R, ..(4名)
    • Journal Title

      Cell

      Volume: 171 Issue: 2 Pages: 287-304

    • DOI

      10.1016/j.cell.2017.09.030

    • NAID

      120006353839

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quantitative confocal imaging method for analyzing cellulose dynamics during cell-wall regeneration in Arabidopsis mesophyll protoplasts.2017

    • Author(s)
      Kuki H, Higaki T, Yokoyama R, Kuroha T, Shinohara N, Hasezawa S, Nishitani K
    • Journal Title

      Plant Direct

      Volume: 1 Issue: 6 Pages: 1-10

    • DOI

      10.1002/pld3.21

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The plant extracellular enzyme AtXTH3 catalyses covalent cross-linking between cellulose and cello-oligosaccharide2017

    • Author(s)
      Shinohara N, Sunagawa N, Tamura S, Yokoyama R, Ueda M, Igarashi K, Nishitani K
    • Journal Title

      Scientific Reports

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabidopsis regenerating protoplast: A powerful model system for combining the proteomics of cell wall proteins and the visualization of cell wall dynamics2016

    • Author(s)
      Yokoyama, R., Kuki, H., Kuroha, T., Nishitani, K.
    • Journal Title

      Proteomes

      Volume: 4 Issue: 4 Pages: 34-34

    • DOI

      10.3390/proteomes4040034

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ゼニゴケにおける Endoglucanase16 の機能解析2019

    • Author(s)
      石田光南,黒羽剛,石崎公庸,檜垣匠,楢本悟史,横山隆亮,小竹敬久,西谷和彦
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Insight into strategies for plant-plant parasitism in Cuscuta campestris based on genome sequencing2018

    • Author(s)
      Narukawa H, Yokoyama R, Obayashi T, Kaga Y, Kato M, Kuroha T, Nishitani K
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 洪水環境に適応した浮イネの進化におけるジベレリン合成遺伝子の関与2018

    • Author(s)
      黒羽剛,ダイアンワン,スーザンマッコチーチ,横山隆亮,西谷和彦,芦苅基行
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アメリカネナシカズラのゲノム解読による寄生植物の適応進化メカニズムの解明2018

    • Author(s)
      横山隆亮,大林武,鳴川秀樹,加賀悠樹,加藤萌木,黒羽剛,西谷和彦
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アメリカネナシカズラが持つ付着器の形成に関与する遺伝子の探索2018

    • Author(s)
      藤原大輝,横山隆亮,西谷和彦,青木考
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular insights into changes of the extracellular matrix during cold and sub-zero acclimation2018

    • Author(s)
      Takahashi D, Sampathkumar A, Erban A, Gorka M, Kopka J, Graf A, Yokoyama R, Kuroha T, Nishitani K, Zuther E, Hincha DK
    • Organizer
      11th International Plant Cold Hardiness Seminar (IPCHS)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 浮イネにおけるジベレリン合成を介した洪水への適応戦略2018

    • Author(s)
      黒羽剛,増口潔,瀬戸義哉,山口信次郎,横山隆亮,西谷和彦
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物種間情報伝達を介したアメリカネナシカズラ吸器における維管束新生機構の探索2018

    • Author(s)
      加賀悠樹,大林武,横山隆亮,黒羽剛,大谷美沙都,出村拓,西谷和彦
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 寄生植物アメリカネナシカズラの花成制御におけるFTの機能2018

    • Author(s)
      加藤萌木,横山隆亮,黒羽剛,大林武,阿部光知,西谷和彦
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 基部陸上植物における細胞壁酵素(GH16)の進化2018

    • Author(s)
      石田光南,黒羽剛,石崎公庸,檜垣匠,楢本悟史,横山隆亮,西谷和彦
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 炭素イオンビームによる寄生植物ネナシカズラ属の変異体ライブラリ作製2018

    • Author(s)
      横山隆亮,加賀悠樹,加藤萌木,古井瑛恵,黒羽剛,西谷和彦,佐藤勝也、大野豊
    • Organizer
      QST 高崎サイエンスフェスタ2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] The C. campestris genome provides new insights into adaptation for the heterotrophic life style in parasitic plants2018

    • Author(s)
      Yokoyama R, Obayashi T, Narukawa H, Kaga Y, Kato M, Kuroha T, Nishitani K
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Insight into strategies for plant-plant parasitism in Cascuta campestris based on genome sequencing2018

    • Author(s)
      Narukawa H, Yokoyama R, Obayashi T, Kaga Y, Kato M, Kuroha T, Nishitani K
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] XTHの新規機能に基づく新しい植物細胞壁像2017

    • Author(s)
      篠原直貴、砂川直輝、田村理、横山隆亮、上田実、五十嵐圭日子、西谷和彦
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] プロトプラスト細胞壁再生系:細胞壁構築プロセスの可視化・定量化のためのツール2017

    • Author(s)
      九鬼寛明、桧垣匠、,横山隆亮、馳澤盛一郎、西谷和彦
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Apoplastic and symplastic interactions between parasitic plants and host plants2017

    • Author(s)
      Aoki K, Hozumi A, Ekawa M, Shimizu K, Yokoyama R, Nishitani K
    • Organizer
      14th World Congress on Parasitic Plants
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ネナシカズラのゲノムから読み解く茎寄生の生命戦略2017

    • Author(s)
      西谷和彦,横山隆亮,大林武,鳴川秀樹,柴田航希,加賀悠樹,加藤萌木,黒羽剛,倉田哲也,堀江佐知子,牧雅之,山田和範,青木裕一,田高周,成瀬孝史,西羽美,木下賢吾,青木考,阿部光知,出村拓,吉田聡子,白須賢 ,長谷部光泰,星野敦,榊原康文,若杉達也,山田恭司
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 比較ゲノム解析から見えてきた茎寄生植物ネナシカズラの形態形成メカニズム2017

    • Author(s)
      横山隆亮,大林武,鳴川秀樹,加賀悠樹,加藤萌木,黒羽剛,西谷和彦
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 茎寄生植物ネナシカズラにおける宿主由来エチレンを介した吸器発生メカニズム2017

    • Author(s)
      鳴川秀樹,加賀悠樹,横山隆亮,黒羽剛,西谷和彦
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 細胞壁イメージングと新規の酵素機能の解析に基づく新しい細胞壁高次構造モデルの提案2017

    • Author(s)
      九鬼寛明,桧垣匠,篠原直貴,横山隆亮,黒羽剛,三上慎吾,馳澤盛一郎,西谷和彦
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Parasitic Plant-Host Interactions Control Endoreduplication-Mediated Cell Expansion During Haustorial Development of the Holoparasitic Plant, Cuscuta Campestris2017

    • Author(s)
      Narukawa H, Kaga Y, Kuroha T, Yokoyama R, Nishitani K
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Quantitative Imaging Approaches to Mechanisms of Cell Wall Construction Using Arabidopsis Mesophyll Protoplasts2017

    • Author(s)
      Kuki H, Higaki T, Yokoyama R, Kuroha T, Hasezawa S, Nishitani K
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] A Model for the Molecular Mechanism of Vascular Development in the Haustorium of the Parasitic Plant Cuscuta Campestris2017

    • Author(s)
      Kaga Y, Obayashi T, Yokoyama R, Nishitani K
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Elucidation of the molecular mechanisms controlling flowering in the stem parasitic plant Cuscuta campestris2017

    • Author(s)
      Kato M, Yokoyama R, Kuroha T, Abe M, Nishitani K
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cell Wall-Related Gene Families in Land Plants2017

    • Author(s)
      Yokoyama R, Kuroha T, Kuki H, Shinohara N, Mikami S, Nishitani K
    • Organizer
      第65回NIBBコンファレンス
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] XTH-mediated covalent-linking between cellulose and cellulose2016

    • Author(s)
      Nishitani K
    • Organizer
      XIV Cell Wall Meeting
    • Place of Presentation
      Chania, Greece
    • Year and Date
      2016-12-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞壁再生イメージング解析法:細胞壁動態解明への新しいアプローチ2016

    • Author(s)
      九鬼寛明、桧垣匠、,横山隆亮、馳澤盛一郎、西谷和彦
    • Organizer
      東北植物学会第6回大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2016-12-10
    • Related Report
      2016 Research-status Report
  • [Presentation] A bioinformatics approach to the identification, classification, and characterization of cell-wall-related gene families yields evolutionary insights into plant cell walls2016

    • Author(s)
      Yokoyama R, Nishitani K
    • Organizer
      XIV Cell Wall Meeting
    • Place of Presentation
      Chania, Greece
    • Year and Date
      2016-06-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 東北大学大学院生命科学研究科植物細胞壁分野ホームページ

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/nishitani_lab/

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Remarks] 東北大大学院生命科学研究科 植物細胞壁機能分野ホームページ

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/nishitani_lab/

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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