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pect1-4 shows growth retardation at low temperature due to downregulation of COP respiration

Research Project

Project/Area Number 16K07392
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionSaitama University

Principal Investigator

NISHIDA IKUO  埼玉大学, 理工学研究科, 教授 (40189288)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords呼吸活性 / 低温成長 / リン脂質生合成の意義 / 呼吸 / COP活性 / AOP活性 / 外向きNAD(P)HデヒドロゲナーゼNDB2 / 低温生育改善 / 恒常的活性型AOX1a* / 光呼吸 / 早期花成 / COP経路 / AOP経路 / COX活性 / 低温生育阻害 / ホスファチジルエタノールアミン / pect1-4 / aox1a / 脂質 / 植物 / 生理学
Outline of Final Research Achievements

The pect1-4 mutant that exhibits a 26% residual activity of CDP-ethanolamine biosynthesis shows pleiotropic mutant phenotypes, such as growth retardation at low temperature or short-day conditions and early flowering. In this study, we demonstrated that pect1-4 mutation causes downregulation of COP respiratory capacity, which in turn causes the growth retardation at low temperature or short-day conditions. Overexpression of constitutively active AOX1a* in pect1-4 mutants fully resumed the COP respiratory capacity and recovered from the growth retardation. On the other hand, pect1-4 AOX1a* plants partially recovered from the early flowering phenotype. Taken together, our results suggested that the pect1-4 mutant exhibits the pleiotropic mutant phenotypes via a respiratory capacity-dependent stress pathway and a non-stress pathway.

Academic Significance and Societal Importance of the Research Achievements

ホスファチジルエタノールアミン(PE)はミトコンドリアなどの細胞内小器官の膜を構成するリン脂質であるが、その生合成を完全に阻害すると、細胞が生存できず、さらなる研究を阻んでいた。われわれは、PE生合成活性が野生型の1/4まで低下したpect1-4変異株を用いることにより、ミトコンドリアの機能低下によってもたらされる変異は、呼吸に依存したストレス経路と、呼吸に依存しない非ストレス経路によって引き起こされることをあきらかにした。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2020 2019 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Eukaryotic lipid metabolic pathway is essential for functional chloroplasts and CO2 and light responses in Arabidopsis guard cells.2018

    • Author(s)
      Negi, J., Munemasa, S., Song, B., Tadakuma, R., Fujita, M., Azoulay-Shemer, T., Engineer, C. B., Kusumi, K., Nishida, I., Schroeder, J. I. and Iba, K.
    • Journal Title

      Proc Natl. Acad. Sci. USA

      Volume: 115 Issue: 36 Pages: 9038-9043

    • DOI

      10.1073/pnas.1810458115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] シロイヌナズナpect1-4変異株における早期花成メカニズムの解析2020

    • Author(s)
      佐藤有季,星野奈摘,生貝咲貴,藤木友紀,西田生郎
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A mechamism of early flowering in pect1-4 mutants of Arabidopsis thaliana2019

    • Author(s)
      Sato, Y., Hoshino, N., Ikegai, S. and Nishida, I.
    • Organizer
      8th Asian-Oceanian Symposium on Plant Lipids (ASPL2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナpect1-4変異株における早期花成メカニズムの解析2019

    • Author(s)
      佐藤有季,星野奈摘,生貝咲貴,西田生郎
    • Organizer
      第32回植物脂質シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 恒常的活性型AOX1a*の過剰発現はpect1-4変異株のCOP活性低下を解消する2019

    • Author(s)
      中沢和音、清水琢登、西田生郎
    • Organizer
      第32回植物脂質シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナpect1-4変異株における早期花成とヒストン修飾に関する研究2018

    • Author(s)
      佐藤有季、星野奈摘、生貝咲貴、藤木友紀、西田生郎
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] シロイヌナズナ種子貯蔵タンパク質変異株における種子特異的BnDGAT1発現の影響2018

    • Author(s)
      Nishida, I., Haneishi, K., Inanobe, A., Sato, A., Kato, A., Lee, Y. and Fujiki, Y.
    • Organizer
      第31回植物脂質シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] シロイヌナズナのPI3P結合タンパク質AtFYVE2のオートファジーと老化葉における役割2018

    • Author(s)
      藤木友紀、岡部茉莉子、Youngsook Lee、西田生郎
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] The CDP-choline Pathway to Phosphatidylcholine Biosynthesis is Required for the Maintenance of Endoplasmic Reticulum at Low Temperature2018

    • Author(s)
      Kuga, C., Sato, Y., Yatake, M. and Nishida, I.
    • Organizer
      The 23rd International Symposium on Plant Lipids
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genetic Disruption of CRC 12S Globulin and Overexpression of BnDGAT1 Synergistically Improve the Seed Oil Content in Arabidopsis2018

    • Author(s)
      Fujiki, Y., Haneishi, K., Sato, A., Kato, A., Lee, Y. and Nishida, I.
    • Organizer
      The 23rd International Symposium on Plant Lipids
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] pect1-4 aox1a-1 Double Mutants Grow Better Than pect1-4 Single Mutants at Low Temperature2018

    • Author(s)
      akuto Shimizu, Ko Noguchi, Ikuo Nishida
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] A mechanism of early flowering in Arabidopsis thaliana pect1-4 mutants2017

    • Author(s)
      Natsumi Hoshino, Saki Ikegai, Mayu Nakagawa, Yuki Fujiki, Ikuo Nishida
    • Organizer
      Japanese Society of Plant Physiologists Annual Meeting
    • Place of Presentation
      鹿児島大学(鹿児島県、鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] pect1-4のCOP活性低下はpect1-4 aox1a-1では解消する2017

    • Author(s)
      清水琢登 矢竹美樹 野口航 西田生郎
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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