Crosstalk regulation of Ca2+ and CO2 signals for bicarbonate transport in algae

• Project/Area Number: 16K07399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Yamano Takashi 京都大学, 生命科学研究科, 講師 (70570167)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: CO2濃縮機構 / クラミドモナス / ピレノイド / 重炭酸イオン輸送 / 光合成 / デンプン鞘 / 重炭酸イオン輸送体 / CO2シグナル伝達 / Ca2+シグナル伝達 / 無機炭素濃縮機構

Outline of Final Research Achievements

In this study, by forward genetic analysis using the green alga Chlamydomonas reinhardtii, we revealed the following points: (1) The calcium-binding protein CAS regulates CCM-related gene expression, including bicarbonate transporters, by retrograde signaling from chloroplast to the nucleus, (2) CAS changes its localization in the chloroplast in response to environmental CO2 concentrations and localizes within a structure called pyrenoid under CO2 deficiency conditions, and (3)Starch sheath formed around pyrenoid is essential for maintaining photosynthetic activity and proper localization of LCIB, an essential factor of the CCM, around the pyrenoid.

Academic Significance and Societal Importance of the Research Achievements

現在の地球環境は、持続可能な開発目標（SDGs）に関わる温暖化・食糧不足・エネルギー枯渇などの様々な問題を抱えている。これに対して、水圏に生息する藻類が持つCO2濃縮機構を利用・改変して、光合成の能力を最大限に高めた陸上植物を創出し、解決しようとする試みが広がっている。例えば、主要作物にCO2濃縮機構を導入し、CO2の吸収量と生産量を高めるといった研究である。本研究課題で明らかになった、藻類のCO2濃縮機構の制御機構に関する新しい知見は、これらの喫緊の課題を解決するためのブレイクスルーにつながることが期待される。

Research Products

• [Journal Article] CrABCA2 Facilitates Triacylglycerol Accumulation in Chlamydomonas reinhardtii under Nitrogen Starvation (2020)
  • Author(s): Jang S, Kong F, Lee J, Choi BY, Wang P, Gao P, Yamano T, Fukuzawa H, Kang BH, Lee Y
  • Journal Title: Molecules and Cells Volume: 43 Pages: 48-57
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal (2020)
  • Author(s): Yamano T, Fukuzawa H
  • Journal Title: Methods in Molecular Biology Volume: 2050 Pages: 155-161
  • DOI: 10.1007/978-1-4939-9740-4_16
  • NAID: 120006767891
  • ISBN: 9781493997398, 9781493997404
  • Peer Reviewed / Open Access
• [Journal Article] The bZIP transcription factor regulates lipid remodeling and contributes to ER stress management in Chlamydomonas reinhardtii. (2019)
  • Author(s): Yamaoka Y, Shin S, Choi BY, Kim H, Jang S, Kajikawa M, Yamano T, Kong F, Legeret B, Fukuzawa H, Li-Beisson Y, Lee Y.
  • Journal Title: Plant Cell Volume: 31 Issue: 5 Pages: 1127-1140
  • DOI: 10.1105/tpc.18.00723
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A practical guide to intelligent image-activated cell sorting (2019)
  • Author(s): Isozaki A, Mikami H, Hiramatsu K, Sakuma S, Kasai Y, Iino T, Yamano T, Yasumoto A, Oguchi Y, Suzuki N, Shirasaki Y, Endo T, Ito T, Hiraki , Yamada M, Matsusaka S, Hayakawa T, Fukuzawa H, Yatomi Y, Arai F, Di Carlo D, Nakagawa A, Hoshino Y, Hosokawa Y, Uemura S, Sugimura T, Ozeki Y, Nitta N, Goda K
  • Journal Title: Nature Protocols Volume: 14 Issue: 8 Pages: 2370-2415
  • DOI: 10.1038/s41596-019-0183-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Lipid remodeling regulator 1 (LRL1) is differently involved in the phosphorus‐depletion response from PSR1 in Chlamydomonas reinhardtii (2019)
  • Author(s): Hidayati Nur A.、Yamada‐Oshima Yui、Iwai Masako、Yamano Takashi、Kajikawa Masataka、Sakurai Nozomu、Suda Kunihiro、Sesoko Kanami、Hori Koichi、Obayashi Takeshi、Shimojima Mie、Fukuzawa Hideya、Ohta Hiroyuki
  • Journal Title: The Plant Journal Volume: 100 Issue: 3 Pages: 610-626
  • DOI: 10.1111/tpj.14473
  • Peer Reviewed / Open Access
• [Journal Article] 緑藻クラミドモナスにおける光合成ターボエンジンの駆動と制御 (2019)
  • Author(s): 山野 隆志、福澤 秀哉
  • Journal Title: 光合成研究 Volume: 29 Pages: 14-28
  • NAID: 120006767894
  • Peer Reviewed
• [Journal Article] Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions (2019)
  • Author(s): Shinkawa H, Kajikawa M, Nomura Y, Ogura M, Sawaragi Y, Yamano T, Nakagami H, Sugiyama N, Ishihama Y, Kanesaki Y, Yoshikawa H, Fukuzawa H
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 4 Pages: 916-930
  • DOI: 10.1093/pcp/pcz010
  • NAID: 120006823940
  • Peer Reviewed / Open Access
• [Journal Article] Inner lumen proteins stabilize doublet microtubules in cilia and flagella (2019)
  • Author(s): Owa M, Uchihashi T, Yanagisawa H, Yamano T, Iguchi H, Fukuzawa H, Wakabayashi K, Ando T, Kikkawa M
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1143-1143
  • DOI: 10.1038/s41467-019-09051-x
  • NAID: 120006591370
  • Peer Reviewed / Open Access
• [Journal Article] Intelligent Image-Activated Cell Sorting (2018)
  • Author(s): Nitta N et al.
  • Journal Title: Cell Volume: 175 Issue: 1 Pages: 266-276.e13
  • DOI: 10.1016/j.cell.2018.08.028
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High-resolution suborganellar localization of Ca2+-binding protein CAS, a novel regulator of CO2-concentrating mechanism (2018)
  • Author(s): Yamano T, Toyokawa C, Fukuzawa H
  • Journal Title: Protoplasma Volume: ー Issue: 4 Pages: 1015-1022
  • DOI: 10.1007/s00709-018-1208-2
  • NAID: 120006482851
  • Peer Reviewed / Open Access
• [Journal Article] Indirect Immunofluorescence Assay in Chlamydomonas reinhardtii. (2016)
  • Author(s): Yamano T, Fukuzawa H
  • Journal Title: Bio-protocol Volume: 6 Issue: 13
  • DOI: 10.21769/bioprotoc.1864
  • NAID: 120006769751
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii (2016)
  • Author(s): L. Wang, T. Yamano, S. Takane, Y. Niikawa, C. Toyokawa, S. Ozawa, R. Tokutsu, Y. Takahashi, J. Minagawa, Y. Kanesaki, H. Yoshikawa, and H. Fukuzawa
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 113 Issue: 44 Pages: 12586-12591
  • DOI: 10.1073/pnas.1606519113
  • NAID: 120006425865
  • Peer Reviewed / Open Access
• [Presentation] 緑藻クラミドモナスのSAGA1変異株は異常なピレノイドの形態だけでなく重炭酸イオン取り込み機能の抑制による影響で高CO2要求性の表現型を示す (2020)
  • Author(s): 嶋村 大亮、新川 友貴、山野 隆志、福澤 秀哉
  • Organizer: 第61回日本植物生理学会年会（大阪大学 吹田キャンパス）
• [Presentation] クラミドモナスにおけるデンプン鞘はCO2濃縮機構に必要である (2020)
  • Author(s): 豊川 知華、山野 隆志、福澤 秀哉
  • Organizer: 第61回日本植物生理学会年会（大阪大学 吹田キャンパス）
• [Presentation] 緑藻クラミドモナスのデンプン鞘はCO2濃縮機構に寄与する (2020)
  • Author(s): 山野 隆志、豊川 知華、福澤 秀哉
  • Organizer: 日本農芸化学会2020年度大会 （九州大学 伊都キャンパス）
• [Presentation] Running and regulation of photosynthetic turbocharger engine in green alga (2019)
  • Author(s): 山野 隆志
  • Organizer: ERATOセミナー（理化学研究所和光キャンパス）
  • Invited
• [Presentation] ピレノイド周囲のデンプン鞘はLCIBの局在と無機炭素濃縮機構に必要である (2019)
  • Author(s): 豊川 知華、山野 隆志、松岡 俊樹、福澤 秀哉
  • Organizer: 第13回クラミドモナス研究会（東京工業大学 すずかけ台キャンパス 大学会館）
• [Presentation] インテリジェント画像活性細胞選抜法によるタンパク質局在変化を指標にした藻類細胞の高速変異株スクリーニング (2019)
  • Author(s): 山野 隆志、豊川 知華、新田 尚、杉村 武昭、磯崎 瑛宏、飯野 敬矩、伊藤 卓朗、合田 圭介、福澤 秀哉
  • Organizer: 第13回クラミドモナス研究会（東京工業大学 すずかけ台キャンパス 大学会館）
• [Presentation] CO2濃縮に関わるデンプン結合性因子の同定とCRISPR/Cas9による遺伝子破壊 (2019)
  • Author(s): 嶋村 大亮、新川 友貴、山野 隆志、福澤 秀哉
  • Organizer: 第37日本植物細胞分子生物学会大会（京都府立大学 稲盛記念会館）
• [Presentation] 緑藻クラミドモナスのデンプン鞘はLCIBの局在と無機炭素濃縮機構に必要である (2019)
  • Author(s): 豊川 知華、山野 隆志、福澤 秀哉
  • Organizer: ユーグレナ研究会 第35回研究集会（大阪府立大学 I-siteなんば）
• [Presentation] CO2-dependent relocation of calcium-binding protein CAS and LCIB essential for carbon-concentrating mechanism in Chlamydomonas reinhardtii (2018)
  • Author(s): Takashi Yamano, Chihana Toyokawa, Toshiki Matsuoka, Hideya Fukuzawa
  • Organizer: 18th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Int'l Joint Research
• [Presentation] Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii (2018)
  • Author(s): Daisuke Shimamura, Yuki Niikawa, Donghui Hu, Asuka Miyamoto, Tomoya Honjo, Noriko Kozai, Chihana Toyokawa, Takashi Yamano, Hideya Fukuzawa
  • Organizer: The 17th International Joint Mini-Symposium on Molecular and Cell Biology among Kyoto University, National Taiwan University, the University of Tsukuba
  • Int'l Joint Research
• [Presentation] 緑藻クラミドモナスのCO2濃縮機構に必須な因子LCIBの局在制御機構 (2018)
  • Author(s): 豊川 知華，松岡 俊樹，西村 明洋，山野 隆志，福澤 秀哉
  • Organizer: 日本植物学会第82回大会
• [Presentation] 松岡 俊樹、豊川 知華、山野 隆志、福澤 秀哉 (2018)
  • Author(s): CO2濃縮に必要な因子LCIBがピレノイド周囲に局在する培養条件の再検討
  • Organizer: 日本植物学会第82回大会
• [Presentation] 緑藻CO2要求性変異株の単離とCO2濃縮機構の制御因子の大規模探索 (2018)
  • Author(s): 嶋村 大亮、新川 友貴、胡 東輝、本庄 智也、香西 紀子、山野 隆志、福澤 秀哉
  • Organizer: 日本植物学会第82回大会
• [Presentation] CO2要求性変異株の単離とCO2濃縮機構の制御因子の大規模探索 (2018)
  • Author(s): 新川 友貴、胡 東輝、嶋村 大亮、本庄 智也、香西 紀子、宮本 明日香、豊川 知華、山野 隆志、福澤 秀哉
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 緑藻クラミドモナスにおいて光とCO2により制御されるカルシウム結合タンパク質CASの細胞内局在 (2017)
  • Author(s): 王 連勇、山野隆志、新川 友貴、豊川 知華、福澤 秀哉
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 群元キャンパス
  • Year and Date: 2017-03-16
• [Presentation] 葉緑体Ca2+結合タンパク質CASおよびCa2+シグナルを介してCO2応答性遺伝子を制御し光合成無機炭素親和性を維持する (2017)
  • Author(s): 新川 友貴、胡 東輝、王 連勇、山野 隆志、吉川 博文、兼崎 友、福澤 秀哉
  • Organizer: 第8回日本光合成学会年会およびシンポジウム
• [Presentation] 藻類のCO2濃縮とその制御 ー光合成のターボエンジンはいかにして駆動するかー (2017)
  • Author(s): 山野 隆志
  • Organizer: 第13回植物縦横無尽の会
  • Invited
• [Presentation] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS (2017)
  • Author(s): Takashi Yamano
  • Organizer: 第19回京都大学生命科学研究科シンポジウム
  • Invited
• [Presentation] 葉緑体Ca2+結合タンパク質CASによる核のCO2応答性遺伝子発現とCO2濃縮の制御 (2017)
  • Author(s): 山野 隆志、新川 友貴、豊川 知華、王 連勇、兼崎 友、吉川 博文、福澤 秀哉
  • Organizer: 日本植物学会第81回大会
• [Presentation] Identification of nuclear genes regulated by chloroplast calcium-sensing receptor homologue, CAS, under CO2-limiting conditions by transcriptome analyses in Chlamydomonas reinhardtii (2016)
  • Author(s): Yuki Niikawa, Lianyong Wang, Takashi Yamano, Hideya Fukuzawa
  • Organizer: The IXth International Symposium on Inorganic Carbon Utilization by Aquatic Photosynthetic Organisms (CCM9)
  • Place of Presentation: Clare College, Cambridge, UK
  • Year and Date: 2016-08-14
  • Int'l Joint Research
• [Presentation] Suborganellar localization of chloroplast Ca2+-binding protein CAS, a novel regulator of CO2-concentrating mechanism, in Chlamydomonas reinhardtii (2016)
  • Author(s): Chihana Toyokawa, Takashi Yamano, Lianyong Wang, Hideya Fukuzawa
  • Organizer: The IXth International Symposium on Inorganic Carbon Utilization by Aquatic Photosynthetic Organisms (CCM9)
  • Place of Presentation: Clare College, Cambridge, UK
  • Year and Date: 2016-08-14
  • Int'l Joint Research
• [Presentation] Characterization of cooperative bicarbonate transport uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii (2016)
  • Author(s): Takashi Yamano, Emi Sato, Hiro Iguchi, Yuri Fukuda, Hideya Fukuzawa
  • Organizer: The IXth International Symposium on Inorganic Carbon Utilization by Aquatic Photosynthetic Organisms (CCM9)
  • Place of Presentation: Clare College, Cambridge, UK
  • Year and Date: 2016-08-14
  • Int'l Joint Research
• [Presentation] Thylakoid calcium-binding protein CAS and calcium regulate the inorganic carbon uptake in Chlamydomonas reinhardtii (2016)
  • Author(s): Lianyong Wang, Takashi Yamano, Shunsuke Takane, Yuki Niikawa, Chihana Toyokawa, Ryutaro Tokutsu, Jun Minagawa, Hideya Fukuzawa
  • Organizer: The IXth International Symposium on Inorganic Carbon Utilization by Aquatic Photosynthetic Organisms (CCM9)
  • Place of Presentation: Clare College, Cambridge, UK
  • Year and Date: 2016-08-14
  • Int'l Joint Research
• [Presentation] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii (2016)
  • Author(s): Takashi Yamano, Lianyong Wang, Shunsuke Takane, Yuki Niikawa, Chihana Toyokawa, Ryutaro Tokutsu, Jun Minagawa, Hideya Fukuzawa
  • Organizer: Satellite Meeting on Photosynthesis
  • Place of Presentation: Kyoto University Seminar House
  • Year and Date: 2016-07-01
  • Int'l Joint Research / Invited
• [Presentation] Thylakoid calcium-binding protein CAS and calcium regulate the expression of ABC-type plasma membrane bicarbonate transporter in Chlamydomonas reinhardtii (2016)
  • Author(s): Lianyong Wang, Takashi Yamano, Shunsuke Takane, Yuki Niikawa, Chihana Toyokawa, Ryutaro Tokutsu, Jun Minagawa, Hideya Fukuzawa
  • Organizer: 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Koyo International Conference Center, Kyoto, JAPAN
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Suborganellar localization of chloroplast Ca2+-binding protein CAS, a novel regulator of CO2-concentrating mechanism in Chlamydomonas reinhardtii (2016)
  • Author(s): Chihana Toyokawa, Takashi Yamano, Lianyong Wang, Jun Minagawa, Hideya Fukuzawa
  • Organizer: 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Koyo International Conference Center, Kyoto, JAPAN
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Identification of nuclear genes regulated by chloroplast calcium-sensing receptor homologue, CAS, under CO2-limiting conditions by transcriptome analyses in Chlamydomonas reinhardtii (2016)
  • Author(s): Yuki Niikawa, Lianyong Wang, Takashi Yamano, and Hideya Fukuzawa
  • Organizer: 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Koyo International Conference Center, Kyoto, JAPAN
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Cooperative bicarbonate uptake into chloroplast stroma by HLA3 and LCIA in Chlamydomonas reinhardtii (2016)
  • Author(s): Takashi Yamano, Emi Sato, Hiro Iguchi, Yuri Fukuda, Hideya Fukuzawa
  • Organizer: 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Koyo International Conference Center, Kyoto, JAPAN
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Ca2+結合タンパク質CASによる葉緑体を介した重炭酸イオン輸送の制御機構 (2016)
  • Author(s): 山野 隆志、王 連勇、高根 俊輔、新川 友貴、豊川 知華、小澤 真一郎、得津 隆太郎、高橋 裕一郎、皆川 純、兼崎 友、吉川 博文、福澤 秀哉
  • Organizer: 第7回日本光合成学会年会およびシンポジウム
  • Place of Presentation: 東京理科大学葛飾キャンパス
  • Year and Date: 2016-05-27
• [Book] 京大発！ フロンティア生命科学 (2018)
  • Author(s): 京都大学大学院生命科学研究科
  • Total Pages: 336
  • Publisher: 講談社サイエンティフィク
  • ISBN: 4065038014
• [Book] 京大発！フロンティア生命科学「18章 体の設計図を読む ゲノム情報と進化」 (2018)
  • Author(s): 山野隆志（編：京都大学大学院生命科学研究科）
  • Total Pages: 336
  • Publisher: 講談社サイエンティフィク
  • ISBN: 9784065038017
• [Book] 微細藻の光合成を支える重炭酸イオン輸送 ー細胞膜と葉緑体包膜に局在する重炭酸イオン輸送体の発見ー (2016)
  • Author(s): 山野隆志、福澤秀哉
  • Total Pages: 2
  • Publisher: 日本農芸化学会
• [Remarks] 京都大学微生物細胞機構学研究室ホームページ
  • URL: http://www.molecule.lif.kyoto-u.ac.jp/index.html
• [Remarks] 「光合成のターボエンジン」CO2濃縮機構が葉緑体を介して制御される仕組みを新たに発見
  • URL: http://www.kyoto-u.ac.jp/ja/research/research_results/2016/161017_1.html