Crosstalk regulation of Ca2+ and CO2 signals for bicarbonate transport in algae
| Project/Area Number |
16K07399
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
Yamano Takashi 京都大学, 生命科学研究科, 講師 (70570167)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | CO2濃縮機構 / クラミドモナス / ピレノイド / 重炭酸イオン輸送 / 光合成 / デンプン鞘 / 重炭酸イオン輸送体 / CO2シグナル伝達 / Ca2+シグナル伝達 / 無機炭素濃縮機構 |
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| Outline of Final Research Achievements |
In this study, by forward genetic analysis using the green alga Chlamydomonas reinhardtii, we revealed the following points: (1) The calcium-binding protein CAS regulates CCM-related gene expression, including bicarbonate transporters, by retrograde signaling from chloroplast to the nucleus, (2) CAS changes its localization in the chloroplast in response to environmental CO2 concentrations and localizes within a structure called pyrenoid under CO2 deficiency conditions, and (3)Starch sheath formed around pyrenoid is essential for maintaining photosynthetic activity and proper localization of LCIB, an essential factor of the CCM, around the pyrenoid.
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| Academic Significance and Societal Importance of the Research Achievements |
現在の地球環境は、持続可能な開発目標（SDGs）に関わる温暖化・食糧不足・エネルギー枯渇などの様々な問題を抱えている。これに対して、水圏に生息する藻類が持つCO2濃縮機構を利用・改変して、光合成の能力を最大限に高めた陸上植物を創出し、解決しようとする試みが広がっている。例えば、主要作物にCO2濃縮機構を導入し、CO2の吸収量と生産量を高めるといった研究である。本研究課題で明らかになった、藻類のCO2濃縮機構の制御機構に関する新しい知見は、これらの喫緊の課題を解決するためのブレイクスルーにつながることが期待される。
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Report
(5 results)
Research Products
(46 results)
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[Journal Article] The bZIP transcription factor regulates lipid remodeling and contributes to ER stress management in Chlamydomonas reinhardtii.2019
Author(s)
Yamaoka Y, Shin S, Choi BY, Kim H, Jang S, Kajikawa M, Yamano T, Kong F, Legeret B, Fukuzawa H, Li-Beisson Y, Lee Y.
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Journal Title
Plant Cell
Volume: 31
Issue: 5
Pages: 1127-1140
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A practical guide to intelligent image-activated cell sorting2019
Author(s)
Isozaki A, Mikami H, Hiramatsu K, Sakuma S, Kasai Y, Iino T, Yamano T, Yasumoto A, Oguchi Y, Suzuki N, Shirasaki Y, Endo T, Ito T, Hiraki , Yamada M, Matsusaka S, Hayakawa T, Fukuzawa H, Yatomi Y, Arai F, Di Carlo D, Nakagawa A, Hoshino Y, Hosokawa Y, Uemura S, Sugimura T, Ozeki Y, Nitta N, Goda K
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Journal Title
Nature Protocols
Volume: 14
Issue: 8
Pages: 2370-2415
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions2019
Author(s)
Shinkawa H, Kajikawa M, Nomura Y, Ogura M, Sawaragi Y, Yamano T, Nakagami H, Sugiyama N, Ishihama Y, Kanesaki Y, Yoshikawa H, Fukuzawa H
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Journal Title
Plant and Cell Physiology
Volume: 60
Issue: 4
Pages: 916-930
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii2016
Author(s)
L. Wang, T. Yamano, S. Takane, Y. Niikawa, C. Toyokawa, S. Ozawa, R. Tokutsu, Y. Takahashi, J. Minagawa, Y. Kanesaki, H. Yoshikawa, and H. Fukuzawa
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 113
Issue: 44
Pages: 12586-12591
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii2018
Author(s)
Daisuke Shimamura, Yuki Niikawa, Donghui Hu, Asuka Miyamoto, Tomoya Honjo, Noriko Kozai, Chihana Toyokawa, Takashi Yamano, Hideya Fukuzawa
Organizer
The 17th International Joint Mini-Symposium on Molecular and Cell Biology among Kyoto University, National Taiwan University, the University of Tsukuba
Related Report
Int'l Joint Research
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