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Regulatory mechanism of growth polarity of plant cells by NIMA-related kinases

Research Project

Project/Area Number 16K07403
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionOkayama University

Principal Investigator

Motose Hiroyasu  岡山大学, 自然科学研究科, 准教授 (70342863)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords成長極性 / NIMA関連キナーゼ / 微小管 / 植物細胞の伸長 / 器官形成 / メカニカルフィードバック / 進化的保存性 / 植物細胞 / 伸長極性 / チューブリン / シロイヌナズナ / ゼニゴケ / キナーゼ / 極性成長 / 先端成長 / 細胞極性 / リン酸化 / 細胞伸長
Outline of Final Research Achievements

We analyzed biological function of NIMA-related kinases (NEK) to address microtubule-dependent growth polarity in plants. Arabidopsis NEK6 localizes to shrinking ends of microtubules and phosphorylates five amino acid residues of beta-tubulin, which induces depolymerization of aberrant microtubules (Takatani et al. 2017 Sci. Rep.). Recently, we found novel function of NEK6 in organ growth. NEK6 curbs microtubule response to tensile stress during organ development to buffer growth variation and to suppress organ deformation (Takatani et al. 2020 Curr. Biol.). Using a basal land plant Marchantia polymorpha, we revealed that NEK-microtubule regulatory module is evolutionary conserved. Marchantia MpNEK1 localizes to the apical dome of rhizoids to stabilize the direction of tip growth of rhizoids (Otani et al. 2018 Development).

Academic Significance and Societal Importance of the Research Achievements

本研究により、これまで不明であったNEKによる成長極性制御機構が明らかになった。NEKは変形した微小管や張力に応答した微小管を選択的に除去し、植物の成長方向を安定化する。この機構は細胞レベルだけでなく、植物の器官成長や姿勢制御においても重要であるという独創的な発見につながった。NEK6が欠損すると、微小管が張力方向に配向して固定化され、局所的な形の歪みが増幅される。本研究で見出された機構を利用することで、植物の成長を制御する新規な技術を開発することができる。また、メタボや加齢による体の変形の抑制、ロボットや人工衛星の姿勢制御、建造物の力学などについて、新たな方法を提供する。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (57 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (7 results) Journal Article (15 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 14 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (32 results) (of which Int'l Joint Research: 9 results,  Invited: 4 results) Remarks (3 results)

  • [Int'l Joint Research] ENS-Lyon(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of British Columbia(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ENS Lyon(フランス)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Univ. British Columbia(Canada)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] ENS Lyon(France)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] ENS-Lyon(France)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] The University of British Columbia(Canada)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Microtubule response to tensile stress is curbed by NEK6 to buffer growth variation in the Arabidopsis hypocotyl2020

    • Author(s)
      Shogo Takatani, Stephane Verger, Takashi Okamoto, Taku Takahashi, Olivier Hamant, Hiroyasu Motose
    • Journal Title

      Current Biology

      Volume: 30 Issue: 8 Pages: 1491-1503

    • DOI

      10.1016/j.cub.2020.02.024

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Chemical synthesis and biological effect on xylem formation of xylemin and Its analogues2020

    • Author(s)
      Hiroyoshi Takamura, Hiroyasu Motose, Taichi Otsu, Shiori Shinohara, Ryugo Kouno, Isao Kadota, Taku Takahashi
    • Journal Title

      Eur. J. Org. Chem.

      Volume: 2020 Issue: 18 Pages: 2745-2753

    • DOI

      10.1002/ejoc.202000322

    • NAID

      120006999366

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nonsense-mediated mRNA decay deficiency affects the auxin response and shoot regeneration in Arabidopsis2019

    • Author(s)
      Chiam NC, Fujimura T, Sano R, Akiyoshi N, Hiroyama R, Watanabe Y, Motose H, Demura T, Ohtani M
    • Journal Title

      Plant Cell Physiol.

      Volume: 60 Issue: 9 Pages: 2000-2014

    • DOI

      10.1093/pcp/pcz154

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Salt hypersensitivity is associated with excessive xylem development in a thermospermine-deficient mutant of Arabidopsis thaliana2019

    • Author(s)
      Shinohara S, Okamoto T, Motose H, Takahashi T
    • Journal Title

      Plant J.

      Volume: 100 Issue: 2 Pages: 374-383

    • DOI

      10.1111/tpj.14448

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of thermospermine on the growth and expression of polyamine-related genes in rice seedlings2019

    • Author(s)
      Miyamoto M, Shimao S, Tong W, Motose H, Takahashi T
    • Journal Title

      Plants

      Volume: 8 Issue: 8 Pages: 296-296

    • DOI

      10.3390/plants8080269

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complexity and Conservation of Thermospermine-Responsive uORFs of SAC51 Family Genes in Angiosperms2019

    • Author(s)
      Soichi Ishitsuka, Mai Yamamoto, Minaho Miyamoto, Yoshitaka Kuwashiro, Akihiro Imai, Hiroyasu Motose, Taku Takahashi
    • Journal Title

      Frontiers in Plant Science

      Volume: 564 Pages: 1-9

    • DOI

      10.3389/fpls.2019.00564

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An evolutionarily conserved NIMA-related kinase directs rhizoid tip growth in the basal land plant Marchantia polymorpha2018

    • Author(s)
      Otani Kento、Ishizaki Kimitsune、Nishihama Ryuichi、Takatani Shogo、Kohchi Takayuki、Takahashi Taku、Motose Hiroyasu
    • Journal Title

      Development

      Volume: 145 Issue: 5 Pages: 154617-154617

    • DOI

      10.1242/dev.154617

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Omeprazole enhances mechanical stress-induced root growth reduction in Arabidopsis thaliana2018

    • Author(s)
      T. Okamoto, S. Takatani, Y. Noutoshi, H. Motose, and T. Takahashi
    • Journal Title

      Plant Cell Physiol.

      Volume: 59 Issue: 8 Pages: 1581-1591

    • DOI

      10.1093/pcp/pcy131

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 植物におけるサーモスペルミンの2つの機能2018

    • Author(s)
      篠原志桜里・本瀬宏康・高橋卓
    • Journal Title

      日本ポリアミン学会誌

      Volume: 5 Pages: 41-46

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Directional cell expansion requires NIMA-related kinase 6 (NEK6)-mediated cortical microtubule destabilization2018

    • Author(s)
      Takatani S, Ozawa S, Yagi N, Hotta T, Hashimoto T, Takahashi Y, Takahashi T, Motose H
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-18

    • DOI

      10.1038/s41598-017-08453-5

    • NAID

      120006337929

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The ARM domain of ARMADILLO-REPEAT KINESIN 1 is not required for microtubule catastrophe but can negatively regulate NIMA-RELATED KINASE 6 in Arabidopsis thaliana2017

    • Author(s)
      Eng RC, Halat LS, Livingston SJ, Sakai T, Motose H, Wasteneys GO
    • Journal Title

      Plant & Cell Physiology

      Volume: 58 Issue: 8 Pages: 1350-1363

    • DOI

      10.1093/pcp/pcx070

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Chemical control of xylem differentiation by thermospermine, xylemin, and auxin.2016

    • Author(s)
      K. Yoshimoto, H. Takamura, I. Kadota, H. Motose and T. Takahashi
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 21487-21487

    • DOI

      10.1038/srep21487

    • NAID

      120005728629

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Polyamine resistance is increased by mutations in a nitrate transporter gene NRT1.3 (AtNPF6.4) in Arabidopsis thaliana.2016

    • Author(s)
      Tong W, Imai A, Tabata R, Shigenobu S, Yamaguchi K, Yamada M, Hasebe M, Sawa S, Motose H, Takahashi T
    • Journal Title

      Front. Plant Sci.

      Volume: 7-834 Pages: 834-834

    • DOI

      10.3389/fpls.2016.00834

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The SAC51 family plays a central role in thermospermine responses in Arabidopsis.2016

    • Author(s)
      Cai Q, Fukushima H, Yamamoto M, Ishii N, Sakamoto T, Kurata T, Motose H, Takahashi T
    • Journal Title

      Plant Cell Physiol.

      Volume: 57 Issue: 8 Pages: 1583-1592

    • DOI

      10.1093/pcp/pcw113

    • NAID

      120006348277

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] サーモスペルミン / 木部分化の鍵を握る低分子 ―遺伝子翻訳に関わるその特異な作用機構2016

    • Author(s)
      高橋卓, 本瀬宏康
    • Journal Title

      生物の科学 遺伝

      Volume: 70 Pages: 356-360

    • Related Report
      2016 Research-status Report
  • [Presentation] ゼニゴケのエチレン応答とエチレン関連遺伝子の解析2019

    • Author(s)
      本瀬宏康・片寄明日香・久保 康隆・高橋 卓
    • Organizer
      第60回日本植物生理学会年会(名古屋)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Straight organ growth requires NEK6-dependent dampening of microtubule response to mechanical stress2019

    • Author(s)
      S Takatani, S Verger, T Okamoto, T Takahashi, O Hamant, H Motose
    • Organizer
      第60回日本植物生理学会年会(名古屋)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 異所的な分裂組織を形成する新奇ゼニゴケ変異体eda1の解析2019

    • Author(s)
      毛利遊野・大谷健人・山岡尚平・西浜竜一・河内孝之・高橋卓・本瀬宏康
    • Organizer
      第60回日本植物生理学会年会(名古屋)
    • Related Report
      2019 Annual Research Report
  • [Presentation] NIMA-related kinases direct plant growth through microtubule organization2019

    • Author(s)
      H Motose
    • Organizer
      John Harada Symposium, UCLA
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ゼニゴケ仮根細胞の先端成長に必要なアルマジロリピート型キネシンの解析2019

    • Author(s)
      本瀬宏康・神田麻花・大谷健人・高橋卓
    • Organizer
      日本植物学会第83回大会(仙台)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Live imaging of rhizoid growth in Marchantia polymorpha.2019

    • Author(s)
      H Motose, A Kanda, T Takahashi
    • Organizer
      Marchantia Workshop 2019, Sendai
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナNIMA関連キナーゼ6の機能解析2019

    • Author(s)
      橋爪駿・高谷彰吾・高橋卓・本瀬宏康
    • Organizer
      植物細胞骨格研究会 Plant Cytoskeleton 2019(熊本)
    • Related Report
      2019 Annual Research Report
  • [Presentation] ゼニゴケ仮根細胞ライブイメージングの確立とアルマジロリピートキネシンの解析2019

    • Author(s)
      神田麻花・高橋卓・本瀬宏康
    • Organizer
      植物細胞骨格研究会 Plant Cytoskeleton 2019(熊本)
    • Related Report
      2019 Annual Research Report
  • [Presentation] ゼニゴケのエチレン応答とエチレン関連遺伝子の解析2019

    • Author(s)
      本瀬 宏康, 片寄 明日香, 久保 康隆, 高橋 卓
    • Organizer
      日本植物生理学会第60回大会(名古屋)
    • Related Report
      2018 Research-status Report
  • [Presentation] 異所的な分裂組織を形成する新奇ゼニゴケ変異体eda1の解析2019

    • Author(s)
      毛利遊野, 大谷健人, 山岡尚平, 西浜竜一, 河内孝之, 高橋卓, 本瀬宏康
    • Organizer
      日本植物生理学会第60回大会(名古屋)
    • Related Report
      2018 Research-status Report
  • [Presentation] Functional analysis of microtubule-associated genes in Marchantia polymorpha2018

    • Author(s)
      H. Motose, M. Kurata, K. Otani, R. Nishihama, T. Kohchi, T. Takahashi
    • Organizer
      EMBO workshop, Lisbon, Portugal
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 進化的に保存された微小管依存的な極性成長のメカニズム2018

    • Author(s)
      本瀬宏康
    • Organizer
      日本植物学会第82回大会(広島)
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] NIMA関連キナーゼファミリーによる細胞伸長制御の進化的な保存性2018

    • Author(s)
      橋爪駿・高谷彰吾・日渡祐二・坂山英俊・西山智明・高橋卓・本瀬宏康
    • Organizer
      日本植物学会第82回大会(広島)
    • Related Report
      2018 Research-status Report
  • [Presentation] ゼニゴケのエチレン応答とエチレン関連遺伝子の解析2018

    • Author(s)
      片寄明日香・久保康隆・高橋卓・本瀬宏康
    • Organizer
      植物細胞骨格研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] ゼニゴケの細胞分裂関連遺伝子の機能解析2018

    • Author(s)
      倉田元気、大谷健人、西浜竜一、河内孝之、高橋卓、本瀬宏康
    • Organizer
      第59回日本植物生理学会年会(札幌)
    • Related Report
      2017 Research-status Report
  • [Presentation] 枝状突起を形成する新奇ゼニゴケ変異体 eda1の解析2018

    • Author(s)
      毛利遊野、大谷健人、山岡尚平、西浜竜一、河内孝之、高橋卓、本瀬宏康
    • Organizer
      第59回日本植物生理学会年会(札幌)
    • Related Report
      2017 Research-status Report
  • [Presentation] NEK6 による微小管の張力応答の抑制はまっすぐな器官伸長に必要である2018

    • Author(s)
      高谷彰吾、Stephane Verger、岡本崇、高橋卓、Olivier Hamant、本瀬宏康
    • Organizer
      第59回日本植物生理学会年会(札幌)
    • Related Report
      2017 Research-status Report
  • [Presentation] 基部陸上植物ゼニゴケの仮根細胞における微小管依存的な先端成長機構の解析2018

    • Author(s)
      本瀬宏康、大谷健人、石崎公庸、高谷彰吾、西浜竜一、河内孝之、高橋卓
    • Organizer
      第59回日本植物生理学会年会(札幌)
    • Related Report
      2017 Research-status Report
  • [Presentation] NEK6 coordinates organ growth by mechanical signal in Arabidopsis2017

    • Author(s)
      S. Takatani, S. Verger, T. Okamoto, T. Hashimoto, T. Takahashi, O. Hamant, H. Motose
    • Organizer
      Plant Signaling & Behavior 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] NIMA関連キナーゼ6の分裂組織における機能解析2017

    • Author(s)
      高谷彰吾、高橋卓、本瀬宏康
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] ゼニゴケから見た微小管関連遺伝子の機能と進化2017

    • Author(s)
      本瀬宏康、大谷健人、倉田元気、高谷彰吾、石崎公庸、西浜竜一、河内孝之、高橋卓
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] NEK6 coordinates organ growth by mechanical signal in Arabidopsis2017

    • Author(s)
      S. Takatani, S. Verger, T. Okamoto, O. Hamant, T. Hashimoto, T. Takahashi, H. Motose
    • Organizer
      Bilateral Closure Symposium of GDRI Integrative Plant Biology Network Program,
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of eda1, a novel Marchantia polymorpha mutant with ectopic branching protrusions in thallus2017

    • Author(s)
      Y. Mori, K. Otani, S. Yamaoka, R. Nishihama, T. Kohchi, T. Takahashi, H. Motose
    • Organizer
      65th NIBB Conference, Marchantia Workshop 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microtubule-dependent directional growth of rhizoids in Marchantia polymorpha2017

    • Author(s)
      H. Motose, K. Otani, K. Ishizaki, R. Nishihama, T. Kohchi, T. Takahashi
    • Organizer
      65th NIBB Conference, Marchantia Workshop 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 維管束分化のケミカルバイオロジー 新規な植物ホルモン・サーモスペルミンの機能解析2017

    • Author(s)
      本瀬宏康
    • Organizer
      岡山大学・資源植物科学研究所・共同研究拠点ワークショップ『植物体再生技術とその分子基盤』
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] シロイヌナズナNEK6はメカニカルシグナルを介した器官成長統御に関与する2017

    • Author(s)
      高谷彰吾・Verger Stephane・岡本崇・橋本隆・高橋卓・Hamant Olivier・本瀬宏康
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島
    • Related Report
      2016 Research-status Report
  • [Presentation] ゼニゴケ微小管関連遺伝子の機能解析2017

    • Author(s)
      本瀬宏康・大谷健人・石崎公庸・西浜竜一・河内孝之・高橋卓
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島
    • Related Report
      2016 Research-status Report
  • [Presentation] ゲノム編集を用いたゼニゴケ微小管関連遺伝子の機能解析2016

    • Author(s)
      本瀬宏康・大谷健人・石崎公庸・西浜竜一・河内孝之・高橋卓
    • Organizer
      第80回日本植物学会年会
    • Place of Presentation
      那覇
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] A NIMA-related kinase regulates directional tip growth and microtubule stability in rhizoid cells of Marchantia polymorpha2016

    • Author(s)
      K. Otani, K. Ishizaki, R. Nishihama, T. Kohchi, T. Takahashi, H. Motose
    • Organizer
      EMBO symposia “Microtubules: From Atoms to Complex Systems”
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2016-05-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Arabidopsis NEK6 destabilizes cortical microtubules through tubulin phosphorylation to direct cell elongation2016

    • Author(s)
      S. Takatani, S. Ozawa, N. Yagi, T. Hotta, Y. Takahashi, T. Hashimoto, T. Takahashi, H. Motose
    • Organizer
      EMBO symposia “Microtubules: From Atoms to Complex Systems”
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2016-05-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 微小管を介した器官成長におけるNEK6の機能解析2016

    • Author(s)
      高谷省吾・高橋卓・本瀬宏康
    • Organizer
      植物細胞骨格研究会(Plant Cytoskeleton 2016)
    • Place of Presentation
      駒場・東京
    • Related Report
      2016 Research-status Report
  • [Presentation] ゼニゴケ微小管関連遺伝子のフェノーム解析2016

    • Author(s)
      本瀬 宏康
    • Organizer
      植物細胞骨格研究会(Plant Cytoskeleton 2016)
    • Place of Presentation
      駒場・東京
    • Related Report
      2016 Research-status Report
  • [Remarks] Don’t feel the force ~植物が張力応答を抑制し、姿勢を正す仕組みを解明

    • URL

      https://www.okayama-u.ac.jp/tp/release/release_id703.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 植物細胞で進化的に保存された極性成長メカニズムを解明 ーゼニゴケの裏側の仮根細胞のひ・み・つー

    • URL

      https://www.okayama-u.ac.jp/tp/release/release_id525.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 植物細胞が真っすぐ伸びる仕組みを解明 ー細胞骨格を整理整頓するタンパクNEK6の働きを解明ー

    • URL

      https://www.okayama-u.ac.jp/tp/release/release_id485.html

    • Related Report
      2019 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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