• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on molecular basis for vegetative propagation of plant

Research Project

Project/Area Number 16K07408
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto Sangyo University

Principal Investigator

KIMURA Seisuke  京都産業大学, 総合生命科学部, 教授 (40339122)

Research Collaborator FERJANI Ali  
IKEUCHI Momoko  
SAKAMOTO Tomoaki  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords栄養繁殖 / 植物 / 不定芽 / 再生
Outline of Final Research Achievements

We have investigated the mechanism of vegetative propagation in Rorippa aquatica, which is a perennial herbaceous semiaquatic plant. R. aquatica propagate asexually in nature by regeneration from leaf fragments. Morphological and anatomical observations showed that both root and shoot apical meristems emerged from the cutting surface of proximal side of the leaf fragment through cell proliferation at vascular tissue. To understand the underlying molecular mechanism for the regeneration, we performed time-series RNA-seq analyses and showed that many auxin-related genes were differentially expressed between the proximal and distal sides of leaf. The results of quantification of auxins and application experiments of auxins suggested that the accumulation of auxins in the proximal side of leaf fragments by polar auxin transport activates the molecular machinery for regeneration of plantlets in R. aquatica. We also found that cytokine is important for shoot regeneration.

Academic Significance and Societal Importance of the Research Achievements

本研究は、これまで適切なモデルが存在しなかったことにより研究がほとんど進んでいなかった栄養繁殖について、R. aquaticaという新しいモデルを用いて研究することが特色である。本研究により、栄養繁殖のメカニズムについて多くの新たな知見が得られたことに大きな意義がある。また、本研究の成果を応用すれば、栽培作物などに高い再性能を付与できる可能性があり、有用作物の改良にも役に立つ。長期的に見れば優良な性質を持つ個体の大量培養、F1雑種系統の維持、絶滅危惧種の繁殖などにも応用可能であり、農業や園芸などの農学分野や保全生態学の分野で役に立つと期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (42 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (6 results) Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 7 results) Presentation (24 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Remarks (1 results)

  • [Int'l Joint Research] Warwick University/University of Edinburgh(英国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of California, Davis(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 中国科学院水生生物研究所(中国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Warwick University(英国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Warwick University/University of Edinburgh(英国)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] University of California, Davis(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Developmental analyses of divarications in leaves of an aquatic fern Microsorum pteropus and its varieties2019

    • Author(s)
      Miyoshi Saori、Kimura Seisuke、Ootsuki Ryo、Higaki Takumi、Nakamasu Akiko
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 1 Pages: e0210141-e0210141

    • DOI

      10.1371/journal.pone.0210141

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plant Temperature Sensors2018

    • Author(s)
      Sakamoto Tomoaki、Kimura Seisuke
    • Journal Title

      Sensors

      Volume: 18 Issue: 12 Pages: 4365-4365

    • DOI

      10.3390/s18124365

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ser-Gln sites of SOG1 are rapidly hyperphosphorylated in response to DNA double-strand breaks2018

    • Author(s)
      Yoshiyama K. O.、Kimura S.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 13 Issue: 6 Pages: e1477904-e1477904

    • DOI

      10.1080/15592324.2018.1477904

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multichromosomal structure of the onion mitochondrial genome and a transcript analysis2018

    • Author(s)
      Mai Tsujimura, Takakazu Kaneko, Tomoaki Sakamoto, Seisuke Kimura, Masayoshi Shigyo, Hiroshi Yamagishi, Toru Terachi
    • Journal Title

      Mitochondrion

      Volume: -

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative transcriptomics with self-organizing map reveals cryptic photosynthetic differences between two accessions of North American Lake cress2018

    • Author(s)
      Nakayama Hokuto、Sakamoto Tomoaki、Okegawa Yuki、Kaminoyama Kaori、Fujie Manabu、Ichihashi Yasunori、Kurata Tetsuya、Motohashi Ken、Al-Shehbaz Ihsan、Sinha Neelima、Kimura Seisuke
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-15

    • DOI

      10.1038/s41598-018-21646-w

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair2018

    • Author(s)
      Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S and Torii KU
    • Journal Title

      Nature Chem. Biol.

      Volume: 14 Issue: 3 Pages: 299-305

    • DOI

      10.1038/nchembio.2555

    • NAID

      120006473593

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Increased Phosphorylation of Ser-Gln Sites on SUPPRESSOR OF GAMMA RESPONSE1 Strengthens the DNA Damage Response inArabidopsis thaliana2017

    • Author(s)
      Yoshiyama Kaoru Okamoto、Kaminoyama Kaori、Sakamoto Tomoaki、Kimura Seisuke
    • Journal Title

      The Plant Cell

      Volume: 29 Issue: 12 Pages: 3255-3268

    • DOI

      10.1105/tpc.17.00267

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] How Do Plants and Phytohormones Accomplish Heterophylly, Leaf Phenotypic Plasticity, in Response to Environmental Cues2017

    • Author(s)
      Nakayama Hokuto、Sinha Neelima R.、Kimura Seisuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 8 Pages: 1-7

    • DOI

      10.3389/fpls.2017.01717

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Asymmetries in leaf branch are associated with differential speeds along growth axes: A theoretical prediction2017

    • Author(s)
      Akiko Nakamasu, Nobuhiko J. Suematsu, Seisuke Kimura
    • Journal Title

      Developmental Dynamics

      Volume: 246 Issue: 12 Pages: 981-991

    • DOI

      10.1002/dvdy.24587

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rorippa aquatica遺伝子情報データベースの構築2016

    • Author(s)
      坂本智昭、木村成介
    • Journal Title

      京都産業大学総合学術研究所報

      Volume: 11 Pages: 105-113

    • NAID

      110010054160

    • Related Report
      2016 Research-status Report
  • [Journal Article] アブラナ科植物Rorippa aquaticaにみられる葉断面からの栄養繁殖条件の検討2016

    • Author(s)
      天野瑠美、中山北斗、木村成介
    • Journal Title

      京都産業大学総合学術研究所報

      Volume: 11 Pages: 115-121

    • NAID

      110010054161

    • Related Report
      2016 Research-status Report
  • [Presentation] アブラナ科植物Rorippa aquaticaの茎生葉上の新奇分裂組織を用いた栄養繁殖2019

    • Author(s)
      池松朱夏、佐々木亜美、天野瑠美、坂本智昭、木村成介
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rorippa aquaticaの栄養繁殖を調節する植物ホルモンの解析2019

    • Author(s)
      天野瑠美、中山北斗、桃井理沙、郡司玄、竹林裕美子、坂本智昭、笠原博幸、榊原均、Ali Ferjani、木村成介
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 水陸両生植物R. aquaticaの水没に応答した気孔形成抑制メカニズムの解明2019

    • Author(s)
      馬瀬樹志、池松朱夏、野口楓子、坂本智昭、木村成介
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Adaptation of plants to aquatic environments: Studies on heterophylly and vegetative propagation in semi-aquatic plant, Rorippa aquatica2018

    • Author(s)
      Seisuke Kimura, Ikematsu Shuka, Tomoaki Sakamoto, Rumi Amano
    • Organizer
      The 46th Naito Conference on “Mechanisms of Evolution and Biodiversity”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物DNA損傷応答のマスターレギュレーターSOG1が果たす役割2018

    • Author(s)
      愿山(岡本)郁、坂本智昭、上ノ山華織、木村成介
    • Organizer
      日本遺伝学会第90回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rorippa aquaticaを用いた葉断面からの栄養繁殖機構の解析2018

    • Author(s)
      天野瑠美、中山北斗、桃井理沙、郡司玄、竹林裕美子、坂本智昭、笠原博幸、Ali Ferjani、木村成介
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 水陸両生植物Rorippa aquaticaにおける水没に応答した気孔形成抑制メカニズムの解析2018

    • Author(s)
      馬瀬樹志、野口楓子、池松朱夏、坂本智昭、木村成介
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 異形葉性植物Rorippa aquaticaの比較ゲノム解析2018

    • Author(s)
      坂本智昭、木村成介
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rorippa aquaticaの温度移行に応答した葉形決定メカニズムの解析2018

    • Author(s)
      池松朱夏、北野つくし、坂本智昭、笠原博幸、木村成介
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Adaptation of plants to aquatic environments: Studies on vegetative propagation in semi-aquatic plant, Rorippa aquatica2018

    • Author(s)
      Seisuke Kimura
    • Organizer
      第59回日本植物生理学会年会シンポジウム「Amazing Development -Revealing Unusual Developmental Phenomena in Plants 植物が見せるユニークな発生および成長様式を読み解く」
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Rorippa aquaticaの栄養繁殖を正誤する遺伝子群の探索2018

    • Author(s)
      天野瑠美、中山北斗、桃井理沙、郡司玄、竹林裕美子、桶川友季、本橋健、 笠原博幸、Ali Ferjani、木村成介
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 非モデル植物Rorippa aquaticaにみられる栄養繁殖機構の解析2017

    • Author(s)
      天野瑠美、中山北斗、坂本智昭、桃井理沙、郡司玄、Ali Ferjani、木村成介
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県・鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Exploration of genes regulating vegetative propagation in Rorippa aquatica2017

    • Author(s)
      Rumi Amano, Hokuto Nakayama, Risa Momoi, Shizuka Gunji, Yumiko Takebayashi, Yuki Okegawa, Ken Motohashi, Hiroyuki Kasahara, Ali Ferjani, Seisuke Kimura
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Rorippa aquaticaの栄養繁殖を制御する遺伝子群の探索2017

    • Author(s)
      天野瑠美、中山北斗、桃井理沙、郡司玄、竹林裕美子、 笠原博幸、Ali Ferjani、木村成介
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Rorippa aquaticaにおける栄養繁殖とオーキシンの関係の解析2017

    • Author(s)
      桃井理沙、天野瑠美、中山北斗、小島美紀子、竹林裕美子、榊原均、木村成介
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Rorippa aquaticaの栄養繁殖を制御する遺伝子群の探索2017

    • Author(s)
      天野瑠美、中山北斗、桃井理沙、郡司玄、竹林裕美子、 笠原博幸、Ali Ferjani、木村成介
    • Organizer
      日本植物形態学会第29回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Adaptation of plants to aquatic environments: Studies on heterophylly and vegetative propagation in semi-aquatic plant, Rorippa aquatica2017

    • Author(s)
      Seisuke Kimura
    • Organizer
      CSRS seminar
    • Place of Presentation
      RIKEN, CSRS, Yokohama, Japan
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Developmental and molecular studies on the mechanism of vegetative propagateon in Rorippa aquatica2016

    • Author(s)
      Rumi Amano, Hokuto Nakayama, Risa Momoi, Shizuka Gunji, Ali Ferjani, Seisuke Kimura
    • Organizer
      Latest Advances in Plant Development & Environmental Response, 2016 Cold Spring Harbor Asia Conference
    • Place of Presentation
      Awaji, Japan
    • Year and Date
      2016-11-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 水中への適応戦略としての異形葉性と栄養繁殖の進化2016

    • Author(s)
      木村成介
    • Organizer
      植物科学若手研究会2016
    • Place of Presentation
      木江ふれあい郷土資料館(広島県・豊田郡)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Presentation] Rorippa aquaticaの栄養繁殖とオーキシンの関係の解析2016

    • Author(s)
      桃井理沙、天野瑠美、中山北斗、木村成介
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] アブラナ科Rorippa aquaticaを用いた葉断面からの栄養繁殖機構の解2016

    • Author(s)
      天野瑠美、中山北斗、郡司玄、Ali Ferjani、木村成介
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Vegetative propagation in Rorippa aquatica: Understanding plant regeneration using non-model species2016

    • Author(s)
      Rumi Amano, Risa Momoi, Seisuke Kimura
    • Organizer
      The 45th Plant Biotechnology Symposium “International Plant Meeting in Kyoto 2016, Plant Development and Environment-“
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 水中への適応戦略としての異形葉性と栄養繁殖2016

    • Author(s)
      木村成介
    • Organizer
      日本植物学会第80回大会シンポジウム「Induced Development: 環境要因に誘発される発生の多様性と共通性」
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] アブラナ科Rorippa aquaticaを用いた葉断面からの栄養繁殖機構の解析2016

    • Author(s)
      天野瑠美、中山北斗、郡司玄、Ali Ferjani、木村成介
    • Organizer
      日本植物形態学会第28回大会
    • Place of Presentation
      琉球大学(沖縄県・中頭郡)
    • Related Report
      2016 Research-status Report
  • [Remarks]

    • URL

      https://www.seisukekimura.com

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-02-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi