| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We have investigated the mechanism of vegetative propagation in Rorippa aquatica, which is a perennial herbaceous semiaquatic plant. R. aquatica propagate asexually in nature by regeneration from leaf fragments. Morphological and anatomical observations showed that both root and shoot apical meristems emerged from the cutting surface of proximal side of the leaf fragment through cell proliferation at vascular tissue. To understand the underlying molecular mechanism for the regeneration, we performed time-series RNA-seq analyses and showed that many auxin-related genes were differentially expressed between the proximal and distal sides of leaf. The results of quantification of auxins and application experiments of auxins suggested that the accumulation of auxins in the proximal side of leaf fragments by polar auxin transport activates the molecular machinery for regeneration of plantlets in R. aquatica. We also found that cytokine is important for shoot regeneration.
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