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Physiological function of peroxyredoxins in chloroplast ROS-scavenging system

Research Project

Project/Area Number 16K07409
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto Sangyo University

Principal Investigator

MOTOHASHI Takeshi  京都産業大学, 総合生命科学部, 教授 (90301952)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords活性酸素 / 葉緑体 / 酸化ストレス / 光合成 / レドックス制御
Outline of Final Research Achievements

Plants produce abundant oxygen and Reactive Oxygen Species (ROS) by photosynthesis. To protect themselves from oxidative stress, several ROS-scavenging system are identified in chloroplast of plants. Recently, several works indicate that peroxiredoxin-family proteins function in ROS-scavenging system of chloroplast. In this study, we evaluated physiological impacts for peroxiredoxins in ROS-scavenging system of chloroplast.

Academic Significance and Societal Importance of the Research Achievements

ペルオキシレドキシン のROS 消去系に対する生理的な寄与が明らかになれば、葉緑体ROS消去メカニズムで重要な役割を担うシステムが判明し、葉緑体ROS消去系に関する理解が進む。また、陸上植物の葉緑体における抗酸化システムについて、生理的意義の理解を深めることができる。これらのことが明らかになることで、植物の機能改良に向けた技術開発のための基礎的知見となるはずである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2019 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products2019

    • Author(s)
      Ken Motohashi
    • Journal Title

      Sci. Rep.

      Volume: 9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative transcriptomics with self-organizing map reveals cryptic photosynthetic differences between two accessions of North American Lake cress2018

    • Author(s)
      Nakayama Hokuto、Sakamoto Tomoaki、Okegawa Yuki、Kaminoyama Kaori、Fujie Manabu、Ichihashi Yasunori、Kurata Tetsuya、Motohashi Ken、Al-Shehbaz Ihsan、Sinha Neelima、Kimura Seisuke
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-15

    • DOI

      10.1038/s41598-018-21646-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] チオレドキシンシステムによる光合成の調節機構 ~変動する光環境で植物はどのように効率良く光合成を行っているのか?~2018

    • Author(s)
      桶川友季、本橋健
    • Journal Title

      化学と生物

      Volume: 56 Pages: 452-453

    • NAID

      130007665236

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods2017

    • Author(s)
      Ken Motohashi
    • Journal Title

      Biochem. Biophys. Rep.

      Volume: 9 Pages: 310-315

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Seamless Ligation Cloning Extract (SLiCE) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to SLiP Site-Directed Mutagenesis2017

    • Author(s)
      Ken Motohashi
    • Journal Title

      Methods Mol. Biol.

      Volume: 1498 Pages: 349-357

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] m型チオレドキシンは光化学系Iサイクリック電子伝達を負に制御する2019

    • Author(s)
      桶川友季、本橋健
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Regulation of cyclic electron transport around Photosystem I by thioredoxin2018

    • Author(s)
      Yuki Okegawa, Ken Motohashi
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] チオレドキシンによる光化学系Iサイクリック電子伝達の制御メカニズム解析2018

    • Author(s)
      桶川友季、本橋健
    • Organizer
      第9回日本光合成学会年会およびシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Redox regulation of cyclic electron transport around Photosystem I by thioredoxin2018

    • Author(s)
      桶川友季、本橋健
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Analysis of overexpressor of chloroplastic thioredoxins in Arabidopsis thaliana2017

    • Author(s)
      桶川友季、本橋 健
    • Organizer
      第58 回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県・鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods.2017

    • Author(s)
      本橋 健
    • Organizer
      第58 回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県・鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Evaluation of recombinant enzyme-free seamless DNA cloning methods.2017

    • Author(s)
      本橋 健
    • Organizer
      第90回日本生化学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Thioredoxin-dependent redox regulatory system in chloroplasts2016

    • Author(s)
      Ken Motohashi, Yuki Okegawa
    • Organizer
      The 17th International Congress on Photosynthesis Research
    • Place of Presentation
      オランダ・マーストリヒト
    • Year and Date
      2016-08-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2020-03-30  

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