| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
A relaxin-like gonad-stimulating peptide (RGP) of starfish is the first identified invertebrate gonadotropin for final gamete maturation. An antiserum against Patira pectinifera RGP (PpeRGP) was produced by immunizing rabbits. The antiserum was used for the development of a specific and sensitive RIA and ELISA for the measurement of RGP. RGP was present in the radial nerve cords and circumoral nerve rings of P. pectinifera throughout the year and its quantity is mostly the same irrespective of the breeding season. Immunohistochemical observations employing the PpeRGP antibody showed that there was lots of staining in the radial nerve cords and marginal nerves. In contrast, there did not appear to be any staining in the optic cushion. Because there are three orthologs of RGP, RGP derivatives with exchanged each A- and B-chain were synthesized chemically to analyze the interaction of RGP with its receptor. The result suggests that B-chain of RGP is involved in binding to its receptor.
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