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Endocrine mechanisms of adaptation to low temperature in amphibians

Research Project

Project/Area Number 16K07420
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionShizuoka University

Principal Investigator

Okada Reiko  静岡大学, 理学部, 講師 (50386554)

Co-Investigator(Kenkyū-buntansha) 菊山 榮  早稲田大学, 教育・総合科学学術院, 名誉教授 (20063638)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords両生類 / 視床下部 / 下垂体 / 凍結耐性 / アルギニンヴァソトシン / プロラクチン / グルコース / 脳下垂体 / 視床下部ホルモン / 低温適応 / 環境適応
Outline of Final Research Achievements

Using the bullfrog as a model, we studied the discrepancy of the role of hypophysiotropic hormones between poikilotherms and homeotherms. It was revealed that in the frog, the main hypophysiotropic hormones for TSH, ACTH, and PRL are CRF, AVT, and TRH, respectively. Considering that in the poikilothermic frog, TRH secretion is enhanced when subjected to a lower temperature, it was concluded that the hypophysiotropic system for metamorphosing amphibians is reasonable, and that the shift of the role of the hypophysiotropic system would have been one of the driving forces for the appearance of homeotherms. In addition, a novel type of PRL expressed only in the larval bullfrog pituitary is expected to be a clue for analyzing the tolerance mechanism in the larvae to a cold temperature. In another series of experiments, we have demonstrated that in the freeze-resistant Japanese tree frog, a cold temperature enhances synthesis of glucose and tissue-specific expression of glucose transporters.

Academic Significance and Societal Importance of the Research Achievements

視床下部-下垂体の調節メカニズムについて、両生類では哺乳類と異なることの詳細を明らかにした。これは脊椎動物の進化の過程で、下垂体ホルモン産生細胞の視床下部因子に対する感受性が変化したことを示唆する知見であり、比較生物学的・進化学的に意義がある。また、一部の両生類に備わる凍結耐性機構の一端を明らかにし、脊椎動物の多様な生存戦略について考察した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] A novel type of prolactin expressed in the bullfrog pituitary specifically during the larval period2019

    • Author(s)
      Okada Reiko、Suzuki Masakazu、Ito Nozomi、Hyodo Susumu、Kikuyama Sakae
    • Journal Title

      General and Comparative Endocrinology

      Volume: 276 Pages: 77-85

    • DOI

      10.1016/j.ygcen.2019.02.006

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of thyrotropin-releasing hormone receptor 3 in the release of prolactin in the metamorphosing bullfrog larvae2018

    • Author(s)
      Nakano Masaki, Hasunuma Itaru, Minagawa Atsuko, Iwamuro Shawichi, Yamamoto Kazutoshi, Kikuyama Sakae, Machida Takeo, Kobayasi Tetsuya
    • Journal Title

      General and Comparative Endocrinology

      Volume: 267 Pages: 36-44

    • DOI

      10.1016/j.ygcen.2018.05.029

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary2017

    • Author(s)
      Imamichi, Y., Sekiguchi, T., Kitano, T., Kajitani, T., Okada, R., Inaoka, Y., Miyamoto, K., Uwada, J., Takahashi, S., Nemoto, T., Mano, A., Khan, M.R.I., Islam, M.T., Yuhki, K., Kashiwagi, H., Ushikubi, F., Suzuki, N., Taniguchi, T., Yazawa, T.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 8374-8374

    • DOI

      10.1038/s41598-017-08780-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Arginine vasotocin is the major adrenocorticotropic hormone-releasing factor in the bullfrog Rana catesbeiana.2016

    • Author(s)
      Okada. R., Yamamoto, K., Hasunuma, I., Asahina, J., Kikuyama, S.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 237 Pages: 121-130

    • DOI

      10.1016/j.ygcen.2016.08.014

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 両生類の変態:分子から個体レベルの制御2016

    • Author(s)
      岡田令子、鈴木賢一
    • Journal Title

      生物科学

      Volume: 67 Pages: 146-153

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 両生類における下垂体ホルモン放出因子の役割の特異性とその意義2016

    • Author(s)
      岡田令子
    • Journal Title

      比較内分泌学

      Volume: 42 Pages: 60-61

    • NAID

      130005158049

    • Related Report
      2016 Research-status Report
  • [Presentation] 両生類の環境適応とアクアポリン2018

    • Author(s)
      岡田令子,鈴木雅一
    • Organizer
      第43回日本比較内分泌学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ウシガエル幼生下垂体は2種類のプロラクチンを発現している2018

    • Author(s)
      岡田令子,鈴木雅一,伊藤希実,兵藤晋,菊山榮
    • Organizer
      第33回日本下垂体研究会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 両生類をモデルとした腺性下垂体の研究2018

    • Author(s)
      菊山榮
    • Organizer
      第33回日本下垂体研究会学術集会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] アフリカツメガエル3型甲状腺刺激ホルモン放出ホルモン受容体(TRHR3)の構造と機能性発現の検討2018

    • Author(s)
      中野真樹,岩室祥一,小林哲也,山本和俊,菊山榮,蓮沼至
    • Organizer
      第43回日本比較内分泌学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 有尾両生類アカハライモリにおける新規プロラクチン受容体の同定2018

    • Author(s)
      須藤百合子,池田卓聡,岩室祥一,菊山榮,蓮沼至
    • Organizer
      第89回日本動物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アカハライモリ新規プロラクチン受容体遺伝子の同定2018

    • Author(s)
      須藤百合子、池田卓聡、岩室祥一 、菊山榮、蓮沼至
    • Organizer
      日本動物学会関東支部 第70回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 両生類の水環境適応におけるアクアポリンの役割2017

    • Author(s)
      鈴木雅一,岡田令子
    • Organizer
      日本動物学会第88回富山大会 第7回ホメオスタシスバイオロジーシンポジウム
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 変態期のウシガエル幼生下垂体中の2種類のプロラクチンmRNAの発現様式2017

    • Author(s)
      伊藤希実、岡田令子、鈴木雅一、兵頭晋
    • Organizer
      平成29年度日本動物学会中部支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニホンアマガエル凍結耐性におけるグルコースの役割2017

    • Author(s)
      岡田令子,阿達駿,岩﨑良平
    • Organizer
      日本動物学会第88回富山大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ソデフリンに走りアイモリンを得るまで2017

    • Author(s)
      菊山 榮
    • Organizer
      日本動物学会第88回富山大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] プロラクチンはイモリ脳内細胞の分裂活性を高める2017

    • Author(s)
      大和田孝祐、豊田ふみよ、岩室祥一、菊山榮、蓮沼至
    • Organizer
      第42回日本比較内分泌学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] アカハライモリのアルギニンバソトシンV2b受容体の発現とその機能2017

    • Author(s)
      小野慧, 豊田ふみよ, 岩室祥一, 菊山榮, 蓮沼至
    • Organizer
      日本動物学会第69回関東支部大会
    • Place of Presentation
      筑波大学東京キャンパス(東京都文京区)
    • Related Report
      2016 Research-status Report
  • [Presentation] Arginine vasotocin, but not authentic corticotropin-releasing factor, acts as the major factor for inducing the corticotropin release from the bullfrog pituitary.2016

    • Author(s)
      Okada, R., Yamamoto, K., Hasunuma, I., Asahina, J., Kikuyama, S.
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology and 87th meeting of Zoological Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Prolactin acts directly on the thyroid gland of larval bullfrogs to suppress its function2016

    • Author(s)
      Hayama S, Watanabe T, Yamamoto K, Iwamuro S, Kikuyama S, Hasunuma I
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology and 87th meeting of Zoological Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ニホンアマガエルの凍結耐性に関わるグルコース輸送体(GLUT)の機能2016

    • Author(s)
      阿達駿,滝谷優,廣田敦司,鈴木雅一,岡田令子
    • Organizer
      日本動物学会2016中部支部大会
    • Place of Presentation
      静岡大学(静岡市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] 変態に関わる下垂体ホルモンの放出制御因子2016

    • Author(s)
      岡田令子
    • Organizer
      第2回次世代両生類研究会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2016-08-08
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ウシガエル幼生甲状腺のサイロキシン放出におよぼすプロラクチンの影響2016

    • Author(s)
      葉山舜, 山本和俊, 菊山榮, 岩室祥一, 蓮沼至
    • Organizer
      第2回次世代両生類研究会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2016-08-08
    • Related Report
      2016 Research-status Report
  • [Remarks] 岡田研究室 静岡大学理学部生物科学科

    • URL

      https://wwp.shizuoka.ac.jp/bio-okada/

    • Related Report
      2018 Annual Research Report 2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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