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Continuous observation of interaction among cell wall molecules by atomic force microscopy

Research Project

Project/Area Number 16K07423
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionKochi University

Principal Investigator

Mine Ichiro  高知大学, 教育研究部総合科学系黒潮圏科学部門, 准教授 (00274358)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords細胞壁 / 原子間力顕微鏡 / 液中観察 / 巨大細胞性藻類 / 細胞壁マトリックス成分
Outline of Final Research Achievements

Fine structure of cellulose microfibril (CMF) and matrix components present among the gap of CMFs was observed on the inner surface of cell wall fragment cut from three species of giant-celled algae by atomic force microscopy in liquid. Fibrous structures that connect among CMFs arranged in a direction perpendicular to the direction of cell growth in Chara, fine fibrous structures that connect neighboring CMFs and layers of the cell wall of Valonia and granular structures of the cell wall matrix component in Vaucheria were observed. Behavior of these structures in the cell wall under tension should be investigated in future.

Academic Significance and Societal Importance of the Research Achievements

細胞壁の伸びやすさなどの力学的な性質は植物や藻類,菌類における細胞成長の主な要因と考えられており,本研究でその微細な形態が明らかになったマトリックス成分はセルロース微繊維(CMF)との相互作用により細胞の成長を制御していることが予想される。また,これらの微細構造は液中AFM観察という特殊な方法で初めて明らかになったが,人為産物のない生物の真の構造を観察する方法として,今後,生物学の多くの分野で応用されることが予想される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2018 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (1 results)

  • [Journal Article] pH-Dependent Maintenance of Cell Wall Integrity in the Giant-Celled Green Alga <i>Valonia utricularis</i>2018

    • Author(s)
      Ichiro Mine, Sho Suzuki, Kun-Feng Li and Satoko Sekida
    • Journal Title

      CYTOLOGIA

      Volume: 83 Issue: 1 Pages: 99-102

    • DOI

      10.1508/cytologia.83.99

    • NAID

      130006588591

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fibrous matrix component of cell wall in the giant-celled green alga Valonia utricularis observed by atomic force microscopy in liquid2018

    • Author(s)
      Mine I, Sekida S
    • Journal Title

      Protoplasma

      Volume: 255 Issue: 5 Pages: 1575-1579

    • DOI

      10.1007/s00709-018-1251-z

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cell wall layers delimit cell groups derived from cell division in the foliose trebouxiophycean alga Prasiola japonica2018

    • Author(s)
      Mine Ichiro、Kinoshita Urara、Kawashima Shigetaka、Sekida Satoko
    • Journal Title

      Protoplasma

      Volume: 印刷中 Issue: 4 Pages: 1253-1256

    • DOI

      10.1007/s00709-018-1206-4

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 巨大細胞性緑藻バロニアの細胞壁マトリックス成分の液中AFM 観察2016

    • Author(s)
      峯 一朗
    • Organizer
      日本植物学会第80回沖縄大会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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