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Real-time imaging of butterfly wing tissues

Research Project

Project/Area Number 16K07425
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Otaki Joji  琉球大学, 理学部, 准教授 (70360211)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsチョウ / 翅組織 / 色模様形成 / リアルタイムイメージング / 蛍光イメージング / 顕微鏡技術 / イメージング / 昆虫形態 / 昆虫発生
Outline of Final Research Achievements

The objective of this study is to clarify developmental mechanisms of color pattern formation and wing shape formation in butterfly wings through real-time observations of these developmental processes in pupae at the cellular and organelle levels. Focusing on a small butterfly, the pale grass blue butterfly, the experimental system for real-time observations of developmental changes was established using fluorescent dyes that stain nuclei, mitochondria, and membrane structures such as Golgi apparatus. With this system, dynamics of the pupal wing tissues was made clear in detail. Furthermore, it was discovered that color pattern formation in butterfly wings depends not only on physical damage and distortions of the tissue but also on physicochemical characters of contact surface (extracellular matrix) of the tissue.

Academic Significance and Societal Importance of the Research Achievements

チョウの翅形成および色模様形成のメカニズムの解明に一石を投じることができた。そのメカニズムはチョウに限らず、生物の発生全般に適用されうるものであろう。チョウの場合、色模様形成のシグナルは、翅細胞の特定の位置に存在する形成体(オーガナイザー)と呼ばれる小さな特殊な細胞群から発せられると考えられている。形成体からシグナルが発せられるという考え方は100年ほど前に提案され、発生生物学の最も重要な概念の一つであり、現代生物学全体においても重要なパラダイムとなっているが、その実態は未解明のままである。本研究によってその一部が解明され、今後のさらなる発展のための基盤を形成することができた。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2020 2019 2018 2017

All Journal Article (10 results) (of which Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Butterfly eyespot color pattern formation requires physical contact of the pupal wing epithelium with extracellular materials for morphogenic signal propagation2020

    • Author(s)
      Joji M. Otaki
    • Journal Title

      BMC Developmental Biology

      Volume: 20 Issue: 1 Pages: 6-6

    • DOI

      10.1186/s12861-020-00211-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-time in vivo imaging of the developing pupal wing tissues in the pale grass blue butterfly Zizeeria maha: establishing the lycaenid system for multiscale bioimaging.2019

    • Author(s)
      Hirata K, Otaki JM
    • Journal Title

      Journal of Imaging

      Volume: 5 Issue: 4 Pages: 42-42

    • DOI

      10.3390/jimaging5040042

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insights into eyespot color-pattern formation mechanisms from color gradients, boundary scales, and rudimentary eyespots in butterfly wings.2019

    • Author(s)
      Iwata M, Otaki JM
    • Journal Title

      Journal of Insect Physiology

      Volume: 114 Pages: 68-82

    • DOI

      10.1016/j.jinsphys.2019.02.009

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Developmental dynamics of butterfly wings: real-time in vivo whole-wing imaging of twelve butterfly species.2018

    • Author(s)
      Iwata M, Tsutsumi M, Otaki JM
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 16848-16848

    • DOI

      10.1038/s41598-018-34990-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-range effects of wing physical damage and distortion on eyespot color patterns in the hindwing of the blue pansy butterfly Junonia orithya.2018

    • Author(s)
      Otaki JM
    • Journal Title

      Insects

      Volume: 9 Issue: 4 Pages: 195-195

    • DOI

      10.3390/insects9040195

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synergistic damage response of the double-focus eyespot in the hindwing of the peacock pansy butterfly2017

    • Author(s)
      Mayo Iwasaki, Joji M. Otaki
    • Journal Title

      Lepidoptera (InTech)

      Volume: - Pages: 65-82

    • DOI

      10.5772/intechopen.70050

    • ISBN
      9789535136590, 9789535136606
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contact-mediated eyespot color-pattern changes in the peacock pansy butterfly: Contributions of mechanical force and extracellular matrix to morphogenic signal propagation2017

    • Author(s)
      Joji M. Otaki
    • Journal Title

      Lepidoptera (InTech)

      Volume: - Pages: 83-102

    • DOI

      10.5772/intechopen.70098

    • ISBN
      9789535136590, 9789535136606
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] チョウの翅のリアルタイムイメージング:色模様形成の謎に迫る2017

    • Author(s)
      大瀧丈二
    • Journal Title

      昆虫と自然

      Volume: 52(5) Pages: 39-40

    • Related Report
      2017 Research-status Report
  • [Journal Article] チョウの翅の色模様を読み解く:自己相似構造2017

    • Author(s)
      大瀧丈二
    • Journal Title

      昆虫と自然

      Volume: 52(8) Pages: 41-42

    • Related Report
      2017 Research-status Report
  • [Journal Article] Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues2017

    • Author(s)
      Mayo Iwasaki, Joji M. Otaki
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 40705-40705

    • DOI

      10.1038/srep40705

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] チョウの翅発生過程のリアルタイムイメージングによる新知見2019

    • Author(s)
      平田香南子、大瀧丈二
    • Organizer
      日本動物学会第90回大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 色模様決定に機能するチョウの眼状紋オーガナイザー細胞のライブイメージング2018

    • Author(s)
      岩崎茉世、大野良和、大瀧丈二
    • Organizer
      日本動物学会第89回大会
    • Related Report
      2018 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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