| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Coccolithophorids are haptophyte algae which have calcified scales with elaborate morphologies, called coccoliths, on the cell surface. In this study, we aimed to clarify the molecular mechanism of calcification, and obtained the following results, using a coccolithophorid Pleurochrysis haptonemofera: 1) A screening system of calcification-related genes by RNAi has been established. Using the system, candidate genes which had been obtained from cDNA macroarry analysis and transcripts database were screened. 2) In vitro calcification experiments indicated that an SDS-DTT extracted protein and acidic polysaccharide Ph-PS-2 play an important role in crystal nucleation.
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