| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We performed functional analysis of swimming locomotor network of the ascidian larva which are comprised of 5 pairs of cholinergic neurons and 2 pairs of GABAegic/glycinergic neurons by optogenetics and live imaging at the single cell resolution. We also performed single cell transcriptome analysis of ascidian embryos to identify the specific marker genes for individual neurons. We identified the neurons that are essential for start swimming, keep swimming, stop swimming and modulate swimming. We also identified the transcription factors that are essential for the differentiation of individual neurons in the swimming locomotor networks in the ascidian larva by single cell transcriptome analysis.
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