Functional analysis of swimming locomotor network by optogenetics and live imaging at the single cell resolution
| Project/Area Number |
16K07433
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HORIE Takeo 筑波大学, 生命環境系, 助教 (10455925)
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| Project Period (FY) |
2016-10-21 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ホヤ / 遊泳運動 / 運動神経回路 / ニューロン / 光遺伝学 / 神経活動イメージング / ライブイメージング / 神経回路 |
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| Outline of Final Research Achievements |
We performed functional analysis of swimming locomotor network of the ascidian larva which are comprised of 5 pairs of cholinergic neurons and 2 pairs of GABAegic/glycinergic neurons by optogenetics and live imaging at the single cell resolution. We also performed single cell transcriptome analysis of ascidian embryos to identify the specific marker genes for individual neurons. We identified the neurons that are essential for start swimming, keep swimming, stop swimming and modulate swimming. We also identified the transcription factors that are essential for the differentiation of individual neurons in the swimming locomotor networks in the ascidian larva by single cell transcriptome analysis.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、ホヤ幼生の遊泳運動神経回路を構成する一つ一つのニューロンについてその生理的な機能やその発生プログラムを明らかにすることに成功した。その結果、ホヤの各ニューロン遊泳運動神経回路を構成するニューロンは機能的にも、発生的にも脊椎動物の脊髄神経回路と似ていることが明らかとなった。脊髄神経回路は、歩行運動など人の行動に重要な役割をする神経回路である。本研究から、単純なホヤ幼生の遊泳運動神経回路が複雑な脊椎動物の脊髄神経回路を理解するためのシンプルなモデルになり得ることが明らかとなった。
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Report
(5 results)
Research Products
(22 results)
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[Journal Article] GABA-Induced GnRH Release Triggers Chordate Metamorphosis.2020
Author(s)
Hozumi A, Matsunobu S, Mita K, Treen N, Sugihara T, Horie T, Sakuma T, Yamamoto T, Shiraishi A, Hamada M, Satoh N, Sakurai K, Satake H, Sasakura Y.
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Journal Title
Current Biology
Volume: 30
Issue: 8
Pages: 1555-1561
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Conserved gene regulatory module specifies lateral neural borders across bilaterians.2017
Author(s)
Li Y, Zhao D, Horie T, Chen G, Bao H, Chen S, Liu W, Horie R, Liang T, Dong B, Feng Q, Tao Q, Liu X
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 114
Issue: 31
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Calcium imaging and single cell optogenetic analysis of a neural circuit for generating swimming2016
Author(s)
Takeo Horie, Masamichi Ohkura, Kotaro Shimai, Ryoko Horie, Yasunori Sasakura, Takehiro Kusakabe, Junichi Nakai, Michael Levine, Masashi Nakagawa
Organizer
The 22nd International Congress of Zoology
Place of Presentation
Okinawa Institute of Science and Technology, Onna-son, Okinawa, Japan
Related Report
Int'l Joint Research / Invited
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