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Functional analysis of swimming locomotor network by optogenetics and live imaging at the single cell resolution

Research Project

Project/Area Number 16K07433
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionUniversity of Tsukuba

Principal Investigator

HORIE Takeo  筑波大学, 生命環境系, 助教 (10455925)

Project Period (FY) 2016-10-21 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsホヤ / 遊泳運動 / 運動神経回路 / ニューロン / 光遺伝学 / 神経活動イメージング / ライブイメージング / 神経回路
Outline of Final Research Achievements

We performed functional analysis of swimming locomotor network of the ascidian larva which are comprised of 5 pairs of cholinergic neurons and 2 pairs of GABAegic/glycinergic neurons by optogenetics and live imaging at the single cell resolution. We also performed single cell transcriptome analysis of ascidian embryos to identify the specific marker genes for individual neurons. We identified the neurons that are essential for start swimming, keep swimming, stop swimming and modulate swimming. We also identified the transcription factors that are essential for the differentiation of individual neurons in the swimming locomotor networks in the ascidian larva by single cell transcriptome analysis.

Academic Significance and Societal Importance of the Research Achievements

本研究では、ホヤ幼生の遊泳運動神経回路を構成する一つ一つのニューロンについてその生理的な機能やその発生プログラムを明らかにすることに成功した。その結果、ホヤの各ニューロン遊泳運動神経回路を構成するニューロンは機能的にも、発生的にも脊椎動物の脊髄神経回路と似ていることが明らかとなった。脊髄神経回路は、歩行運動など人の行動に重要な役割をする神経回路である。本研究から、単純なホヤ幼生の遊泳運動神経回路が複雑な脊椎動物の脊髄神経回路を理解するためのシンプルなモデルになり得ることが明らかとなった。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (22 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (7 results) Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 6 results) Remarks (4 results)

  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 北京師範大学(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] 清華大学(中国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] 清華大学(中国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] GABA-Induced GnRH Release Triggers Chordate Metamorphosis.2020

    • Author(s)
      Hozumi A, Matsunobu S, Mita K, Treen N, Sugihara T, Horie T, Sakuma T, Yamamoto T, Shiraishi A, Hamada M, Satoh N, Sakurai K, Satake H, Sasakura Y.
    • Journal Title

      Current Biology

      Volume: 30 Issue: 8 Pages: 1555-1561

    • DOI

      10.1016/j.cub.2020.02.003

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Shared evolutionary origin of vertebrate neural crest and cranial placodes.2018

    • Author(s)
      Horie R, Hazbun A, Chen K, Cao C, Levine M, Horie T.
    • Journal Title

      Nature

      Volume: 560 Pages: 228-232

    • NAID

      120007133828

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics.2018

    • Author(s)
      Horie T, Horie R, Chen K, Cao C, Nakagawa M, Kusakabe TG, Sasakura Y, Levine M.
    • Journal Title

      Genes & Development

      Volume: 32 Issue: 19-20 Pages: 1297-1302

    • DOI

      10.1101/gad.317669.118

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Conserved gene regulatory module specifies lateral neural borders across bilaterians.2017

    • Author(s)
      Li Y, Zhao D, Horie T, Chen G, Bao H, Chen S, Liu W, Horie R, Liang T, Dong B, Feng Q, Tao Q, Liu X
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 114 Issue: 31

    • DOI

      10.1073/pnas.1704194114

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 単一細胞トランスクリプトームによるホヤ胚における神経細胞分化を制御する遺伝子カクテルの同定2019

    • Author(s)
      堀江 健生
    • Organizer
      日本動物学会 第90回 大阪大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Regulatory cocktail for individual neurons identified by whole embryos single cell transcriptomics in ascidian embryos2019

    • Author(s)
      Takeo Horie
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Regulatory cocktail for individual neurons in Ciona identified by whole-embryo single-cell transcriptomics2019

    • Author(s)
      Takeo Horie
    • Organizer
      The Ciona Conectome:creating a research network
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 単一細胞トランスクリプトームによる神経細胞の分化を制御する転写因子カクテルの同定2018

    • Author(s)
      堀江 健生
    • Organizer
      阪大学蛋白質研究所セミナー「高次脳機能の神経回路機構」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Neural crest and cranial placodes share a common evolutionary origin2017

    • Author(s)
      Horie T, Horie R, Levine M
    • Organizer
      9th International Tunicate Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Specification of the lateral border of the neural plate in the ascidian embryo2016

    • Author(s)
      Ryoko Horie, Michael Levine, Takeo Horie
    • Organizer
      The 22nd International Congress of Zoology
    • Place of Presentation
      Okinawa Convention Center、Ginowan, Okinawa, Japan
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Calcium imaging and single cell optogenetic analysis of a neural circuit for generating swimming2016

    • Author(s)
      Takeo Horie, Masamichi Ohkura, Kotaro Shimai, Ryoko Horie, Yasunori Sasakura, Takehiro Kusakabe, Junichi Nakai, Michael Levine, Masashi Nakagawa
    • Organizer
      The 22nd International Congress of Zoology
    • Place of Presentation
      Okinawa Institute of Science and Technology, Onna-son, Okinawa, Japan
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 堀江研究室 ホームページ

    • Related Report
      2019 Annual Research Report
  • [Remarks] 筑波大学生命環境系下田臨海実験センター堀江研究室ホームページ

    • Related Report
      2018 Research-status Report
  • [Remarks] 筑波大学生命環境系下田臨海実験センター堀江研究室ホームページ

    • Related Report
      2017 Research-status Report
  • [Remarks] 筑波大学生命環境系下田臨海実験センター堀江研究室ホームページ

    • Related Report
      2016 Research-status Report

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Published: 2016-10-24   Modified: 2024-03-04  

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