Optical analysis of the transport and exocytosis of neuropeptide and their neuromodulatory actions
| Project/Area Number |
16K07435
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Nagoya University |
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Principal Investigator |
Abe Hideki 名古屋大学, 生命農学研究科, 准教授 (90396804)
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| Research Collaborator |
NOGUCHI Akihisa
KAWAMINAMI Haruka
KIYAMA Jyunpei
OOSAKI Maho
KAJIURA Shinji
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 神経ペプチド / 開口放出 / イメージング / 神経修飾 / ペプチド放出 / トランスジェニックメダカ / 神経修飾ペプチド / ペプチド / Ca2+ |
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| Outline of Final Research Achievements |
Using the terminal nerve GnRH3 peptidergic neurons, we analyzed the release dynamics of single peptidergic neuron and its neuromodulatory action of sensory neural process. We found the localization of intracellular Ca 2+ elevation machinery (L-type Ca 2+ channel in soma and neurites, and N-type Ca 2+ channel in distal neurites) and resultant difference of exocytosis in single peptide neuron, We also established a transgenic medaka that specifically expresses synaptopHluorin, an exocytosis sensor, in GnRH3 neurons, and succeeded in visualizing exocytosis from GnRH3 neurons in the brain. We also found that i.v. application of GnRH peptide exerts an acute modulatory action on optokinertic nystagmus of medaka.
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| Academic Significance and Societal Importance of the Research Achievements |
単一ニューロンレベルでの動態が不明であった神経ペプチドの開口放出を、蛍光タンパク質による開口放出センサーを遺伝子導入したトランスジェニックメダカを作成することで、in vitro/ invivo で計測可能とした。これによって脳内におけるペプチド放出動態とその制御機構を解明するための基盤を作り出した。他のペプチドニューロンでも神経ペプチドの放出が神経突起のみならず細胞体からも直接生じることを実測することができ、これらの細胞内領域による放出動態制御は他の神経ペプチド産生ニューロンでもその存在が推測されており、ペプチドニューロンに共通した放出制御機構を探るツールとなる可能性がある。
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Nucleus Ruber of Actinopterygians2016
Author(s)
Nakayama, T., Miyajima, S., Nishino, A., Narita, J., Abe, H., Yamamoto, N.
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Journal Title
Brain Behav. Evol.
Volume: 88
Issue: 1
Pages: 25-42
DOI
Related Report
Peer Reviewed
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[Presentation] Can grass puffersifh (Takifugu niphobles) sniff “Tetrodotoxins”?2016
Author(s)
Noguchi, Y., Sakakibara, R., Satake, Y., Adachi, M., Yamamoto, N., Nishikawa, T., and Abe, H.
Organizer
The 22nd International Congress of Zoology and The 87th Meeting of The Zoological Society of Japan Joint Events
Place of Presentation
Okinawa, Japan
Year and Date
2016-11-14
Related Report
Int'l Joint Research
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