Analysis of uncharacterized phototransduction system in the vertebrate photoreceptor cells

• Project/Area Number: 16K07437
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Kyoto University
• Principal Investigator: Yamashita Takahiro 京都大学, 理学研究科, 助教 (50378535)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 光受容蛋白質 / 視細胞 / 光受容

Outline of Final Research Achievements

Opn5 forms one of the photoreceptive protein, opsin, groups, whose genes are widely distributed in vertebrate genomes. We previously revealed that vertebrate Opn5 is classified into several subgroups and is diversified based on their molecular properties and tissue distribution patterns. In this study, we analyzed one of the subgroups, Opn5n, which is sensitive to visible light and is expressed in the photoreceptor cells. We constructed knock-out medaka fish line and revealed that the fish shows deficient morphological changes in dark-adapted and light-adapted retinas. In addition, we characterized the key amino acid residue(s) responsible for the visible light sensitivity in Opn5n.

Academic Significance and Societal Importance of the Research Achievements

動物は外界の光環境の変化から多様な情報を手に入れている。そのために重要な光センサーがオプシン類である。動物のゲノムでオプシン遺伝子を探索すると、哺乳類は10以下と少ないが、他の脊椎動物ではその倍以上持つものが多い。つまり、哺乳類以外の脊椎動物はより多様なオプシンを用いて、光環境の変化から多彩な情報を手に入れていると考えられる。本研究では、そのようなことに関わる１つのオプシン遺伝子を解析し、哺乳類では見られない光応答システムを明らかにできた。

Research Products

• [Journal Article] Elementary response triggered by transducin in retinal rods. (2019)
  • Author(s): Yue WWS, Silverman D, Ren X, Frederiksen R, Sakai K, Yamashita T, Shichida Y, Cornwall MC, Chen J, Yau KW
  • Journal Title: Proc Natl Acad Sci U S A Volume: 116 Issue: 11 Pages: 5144-5153
  • DOI: 10.1073/pnas.1817781116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Shift in Conformational Equilibrium Induces Constitutive Activity of G-Protein-Coupled Receptor, Rhodopsin. (2018)
  • Author(s): Maeda R, Hiroshima M, Yamashita T, Wada A, Sako Y, Shichida Y, Imamoto Y.
  • Journal Title: J. Phys. Chem. B Volume: 122 Issue: 18 Pages: 4838-4843
  • DOI: 10.1021/acs.jpcb.8b02819
  • NAID: 120006708207
  • Peer Reviewed / Open Access
• [Journal Article] Genotype determination of the OPN1LW/OPN1MW genes: novel disease-causing mechanisms in Japanese patients with blue cone monochromacy. (2018)
  • Author(s): Katagiri S, Iwasa M, Hayashi T, Hosono K, Yamashita T, Kuniyoshi K, Ueno S, Kondo M, Ueyama H, Ogita H, Shichida Y, Inagaki H, Kurahashi H, Kondo H, Ohji M, Hotta Y, Nakano T.
  • Journal Title: Sci Rep Volume: 8 Issue: 1 Pages: 11507-11507
  • DOI: 10.1038/s41598-018-29891-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Single-molecule diffusion-based estimation of ligand effects on G protein?coupled receptors (2018)
  • Author(s): Yanagawa Masataka、Hiroshima Michio、Togashi Yuichi、Abe Mitsuhiro、Yamashita Takahiro、Shichida Yoshinori、Murata Masayuki、Ueda Masahiro、Sako Yasushi
  • Journal Title: Science Signaling Volume: 11 Issue: 548 Pages: 1-16
  • DOI: 10.1126/scisignal.aao1917
  • Peer Reviewed / Open Access
• [Journal Article] Red-Tuning of the Channelrhodopsin Spectrum Using Long Conjugated Retinal Analogues. (2018)
  • Author(s): Shen YC, Sasaki T, Matsuyama T, Yamashita T, Shichida Y, Okitsu T, Yamano Y, Wada A, Ishizuka T, Yawo H, Imamoto Y.
  • Journal Title: Biochemistry Volume: 57 Issue: 38 Pages: 5544-5556
  • DOI: 10.1021/acs.biochem.8b00583
  • NAID: 120006708206
  • Peer Reviewed
• [Journal Article] Pinopsin evolved as the ancestral dim-light visual opsin in vertebrates (2018)
  • Author(s): Keita Sato, Takahiro Yamashita, Keiichi Kojima, Kazumi Sakai, Yuki Matsutani, Masataka Yanagawa, Yumiko Yamano, Akimori Wada, Naoyuki Iwabe, Hideyo Ohuchi, Yoshinori Shichida
  • Journal Title: Communications Biology Volume: 1 Issue: 1 Pages: 156-156
  • DOI: 10.1038/s42003-018-0164-x
  • NAID: 120006528356
  • Peer Reviewed / Open Access
• [Journal Article] Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor (2018)
  • Author(s): Sato Keita、Yamashita Takahiro、Ohuchi Hideyo、Takeuchi Atsuko、Gotoh Hitoshi、Ono Katsuhiko、Mizuno Misao、Mizutani Yasuhisa、Tomonari Sayuri、Sakai Kazumi、Imamoto Yasushi、Wada Akimori、Shichida Yoshinori
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 1255-1255
  • DOI: 10.1038/s41467-018-03603-3
  • NAID: 120006535079
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evolutionary steps involving counterion displacement in a tunicate opsin (2017)
  • Author(s): Kojima Keiichi、Yamashita Takahiro、Imamoto Yasushi、Kusakabe Takehiro G.、Tsuda Motoyuki、Shichida Yoshinori
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 114 Issue: 23 Pages: 6028-6033
  • DOI: 10.1073/pnas.1701088114
  • Peer Reviewed / Open Access
• [Journal Article] Drosophila melanogaster rhodopsin Rh7 is a UV-to-visible light sensor with an extraordinarily broad absorption spectrum. (2017)
  • Author(s): K. Sakai
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 7349-7349
  • DOI: 10.1038/s41598-017-07461-9
  • Peer Reviewed / Open Access
• [Journal Article] Chimeric microbial rhodopsins for optical activation of Gs-proteins (2017)
  • Author(s): K. Yoshida
  • Journal Title: Biophysics and Physicobiology Volume: 14 Issue: 0 Pages: 183-190
  • DOI: 10.2142/biophysico.14.0_183
  • NAID: 130006252951
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Spontaneous activation of visual pigments in relation to openness/closedness of chromophore-binding pocket. (2017)
  • Author(s): W.W. Yue
  • Journal Title: Elife Volume: 6
  • DOI: 10.7554/elife.18492
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Alternative Formation of Red-Shifted Channelrhodopsins: Noncovalent Incorporation with Retinal-Based Enamine-Type Schiff Bases and Mutated Channelopsin (2017)
  • Author(s): T. Okitsu
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 65 Issue: 4 Pages: 356-358
  • DOI: 10.1248/cpb.c17-00054
  • NAID: 130005529782
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed
• [Journal Article] Adaptation of cone pigments found in green rods for scotopic vision through a single amino acid mutation (2017)
  • Author(s): K. Kojima
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Two UV-Sensitive Photoreceptor Proteins, Opn5m and Opn5m2 in Ray-Finned Fish with Distinct Molecular Properties and Broad Distribution in the Retina and Brain. (2016)
  • Author(s): K. Sato
  • Journal Title: PLoS One Volume: 11 Issue: 5 Pages: e0155339-e0155339
  • DOI: 10.1371/journal.pone.0155339
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Two Opsin 3-Related Proteins in the Chicken Retina and Brain: A TMT-Type Opsin 3 Is a Blue-Light Sensor in Retinal Horizontal Cells, Hypothalamus, and Cerebellum. (2016)
  • Author(s): M. Kato
  • Journal Title: PLoS One Volume: 11 Issue: 11 Pages: 1-23
  • DOI: 10.1371/journal.pone.0163925
  • Peer Reviewed / Open Access
• [Presentation] 光で活性化されず不活性化される 非視覚光受容タンパク質Opn5L1 (2018)
  • Author(s): 山下高廣
  • Organizer: 日本動物学会第89回大会
  • Invited
• [Presentation] Revisit of the acquisition process of vertebrate scotopic vision (2018)
  • Author(s): Takahiro Yamashita
  • Organizer: 日本生物物理学会第56回年会
  • Invited
• [Presentation] Pinopsin acts as a scotopic visual pigment in lower vertebrates (2018)
  • Author(s): Takahiro Yamashita
  • Organizer: 18th International Conference on Retinal Proteins
  • Int'l Joint Research
• [Presentation] Blue light-sensitive subtype of vertebrate non-visual opsin Opn5 (2017)
  • Author(s): T. Yamashita
  • Organizer: International Symposium on Biophysics of Rhodopsins
  • Int'l Joint Research / Invited
• [Presentation] Comparison of thermal activation rates between vertebrate visual pigments and pinopsin (2017)
  • Author(s): 山下高廣
  • Organizer: 日本生物物理学会第55回年会
• [Presentation] Diversity of the photoreceptor protein Opn5 found in deuterostomes (2016)
  • Author(s): T. Yamashita
  • Organizer: 日本生物物理学会第54回大会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Analysis of the photoreceptor protein Opn5 found in deuterostomes other than vertebrates (2016)
  • Author(s): T. Yamashita
  • Organizer: 日本動物学会第87回大会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2016-11-17
• [Presentation] Characterization of visible light-sensitive subtype of non-visual opsin, Opn5 (2016)
  • Author(s): T. Yamashita
  • Organizer: 17th International Conference on Retinal Proteins
  • Place of Presentation: ポツダム（ドイツ）
  • Year and Date: 2016-10-05
  • Int'l Joint Research / Invited
• [Remarks] 視覚進化の新モデル ー哺乳類が失った”ピノプシン”からたどる脊椎動物の暗所視と色覚
  • URL: https://academist-cf.com/journal/?p=9119