Study for light response pathways for the circadian clock in grenn algae

• Project/Area Number: 16K07448
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Nagoya University
• Principal Investigator: Matsuo Takuya 名古屋大学, 遺伝子実験施設, 講師 (00452201)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 概日時計 / 緑藻 / 光応答 / クラミドモナス

Outline of Final Research Achievements

We have developed the Chlamydomonas reinhardtii as a model for studying circadian clocks. We found that ROC15, a Chlamydomonas clock protein, undergose light induced degradation. This phenomenon is induced by a wide range of wavelength of light. Interestingly, there are no known photoreceptor that has an absorption spectrum like this. In this study, we identified some new genes involved in this process. This study provide an insight into the evolution of light response pathways in green plant lineage.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、概日時計の人為的制御に繋がります。概日時計は微細藻類のエネルギー代謝の主要な部分（デンプンの蓄積など）に関わっていますので、概日時計の制御は微細藻類の持つ能力を最大限に引き出す手段の一つとなり得ます。また、この光シグナル伝達経路は新奇のものですので、新しい光センサータンパク質の発見や、光受容伝達システムの進化の理解に繋がると期待されます。

Research Products

• [Journal Article] The CONSTANS flowering complex controls the protective response of photosynthesis in the green alga Chlamydomonas (2019)
  • Author(s): Tokutsu Ryutaro、Fujimura-Kamada Konomi、Matsuo Takuya、Yamasaki Tomohito、Minagawa Jun
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 4099-4099
  • DOI: 10.1038/s41467-019-11989-x
  • Peer Reviewed / Open Access
• [Journal Article] Isolation of photoprotective signal transduction mutants by systematic bioluminescence screening in Chlamydomonas reinhardtii. (2019)
  • Author(s): Tokutsu R, Fujimura-Kamada K, Yamasaki T, Matsuo T, Minagawa J
  • Journal Title: Sci Rep. Volume: 9(1) Issue: 1 Pages: 2820-2820
  • DOI: 10.1038/s41598-019-39785-z
  • Peer Reviewed / Open Access
• [Journal Article] ROC75 is an Attenuator for the Circadian Clock that Controls LHCSR3 Expression. (2018)
  • Author(s): Kamrani YY, Matsuo T, Mittag M, Minagawa J
  • Journal Title: Plant Cell Physiol. Volume: 59(12) Pages: 2602-2607
  • DOI: 10.1093/pcp/pcy179
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CSL encodes a leucine-rich-repeat protein implicated in red/violet light signaling to the circadian clock in Chlamydomonas. (2017)
  • Author(s): Kinoshita A, Niwa Y, Onai K, Yamano T, Fukuzawa H, Ishiura M, Matsuo T.
  • Journal Title: PLoS Genet. Volume: 23 Issue: 3 Pages: e1006645-e1006645
  • DOI: 10.1371/journal.pgen.1006645
  • NAID: 120005998025
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Identification of a gene critical for the light induced degradation of circadian clock protein ROC15 in Chlamydomonas reinhardtii (2019)
  • Author(s): Gururaj M, Kinoshita A, Matsuo T
  • Organizer: 第13回クラミドモナス研究会
• [Presentation] 植物オルガネラの概日制御 (2019)
  • Author(s): 松尾拓哉
  • Organizer: 第21回植物オルガネラワークショップ
  • Invited
• [Presentation] 緑藻クラミドモナスの概日時計 (2018)
  • Author(s): 松尾拓哉
  • Organizer: 甲南生物学セミナー
  • Invited
• [Presentation] Functional characterization of two LOV-containing histidine kinases, LHK1 and LHK2, in the green alga Chlamydomonas reinhardtii. (2018)
  • Author(s): Tomida W, Matsuo T, Otsuka T, Suzuki T, Aoki S
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 緑藻クラミドモナスのGARP型DNA結合タンパク質 ROC75の概日時計における役割 (2018)
  • Author(s): 松尾拓哉、飯田高広、武藤梨沙、木下亜有美、石浦正寛
  • Organizer: 日本遺伝学会第90回大会
• [Presentation] A gene crucial for light induced degradation of the clock protein ROC15 in Chlamydomonas reinhardtii (2018)
  • Author(s): Gururaj M, Kinoshita A, Matsuo T
  • Organizer: 日本遺伝学会第90回大会
• [Presentation] Reverse Genetic Approach to Identify Circadian Photoreceptors in Chlamydomonas reinhardtii (2018)
  • Author(s): Cheng JY, Matsuo T
  • Organizer: 日本遺伝学会第90回大会
• [Presentation] クラミドモナスにおける LOV 含有ヒスチジンキナーゼ遺伝子の概日リズムへの機能的関与の検証 (2018)
  • Author(s): 冨田航、松尾拓哉、大塚徹寛、鈴木智紀、青木摂之
  • Organizer: 日本遺伝学会第90回大会
• [Presentation] 緑藻クラミドモナスの概日時計をリセットする光受容伝達機構 (2018)
  • Author(s): Gururaj M、Cheng JY、木下亜有美、松尾拓哉
  • Organizer: 第20回日本光生物学協会年会
• [Presentation] 緑藻クラミドモナスにおける概日時計の光リセット機構 (2018)
  • Author(s): 松尾拓哉、Malavika Gururaj
  • Organizer: 第二回名古屋リズム研究会
• [Presentation] The uncharacterized gene Cre07.g357500 plays a role in light induced degradation of ROC15 in Chlamydomonas reinhardtii (2018)
  • Author(s): Malavika Gururaj, Ayumi Kinoshita, Takuya Matsuo
  • Organizer: 第二回名古屋リズム研究会
• [Presentation] Reverse Genetic Approach to Identify Circadian Photoreceptors in Chlamydomonas reinhardtii (2018)
  • Author(s): Jen-Yin Cheng, Takuya Matsuo
  • Organizer: 第二回名古屋リズム研究会
• [Presentation] 緑藻クラミドモナスのROC75は概日時計の昼位相の制御に重要な因子である (2017)
  • Author(s): 松尾拓哉、飯田高広、武藤梨沙、木下亜有美、石浦正寛
  • Organizer: 日本遺伝学会第89回大会
• [Presentation] 緑藻クラミドモナスの概日時計 (2016)
  • Author(s): 松尾拓哉
  • Organizer: 生物リズム若手研究者の集
  • Place of Presentation: 山口市、湯田温泉、ホテル喜良久
  • Year and Date: 2016-09-03
  • Invited
• [Presentation] Existence of a red/violet light signaling pathway involved in resetting of circadian clock in Chlamydomonas. (2016)
  • Author(s): Ayumi Kinoshita, Takuya Matsuo, Kiyoshi Onai, Takashi Yamano, Hideya Fukuzawa, Masahiro Ishiura
  • Organizer: 17TH INTERNATIONAL MEETING CELL & MOLECULAR BIOLOGY OF CHLAMYDOMONAS
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2016-06-26
  • Int'l Joint Research