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Genetic mechanisms underlying parallel loss of photoperiodism and diversity of seasonal reproduction in sticklebacks

Research Project

Project/Area Number 16K07469
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Evolutionary biology
Research InstitutionNational Institute of Genetics

Principal Investigator

Ishikawa Asano  国立遺伝学研究所, ゲノム・進化研究系, 助教 (20722101)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords季節性繁殖 / 日長応答性 / 甲状腺刺激ホルモン / TSHb2 / トランスクリプトーム解析
Outline of Final Research Achievements

The timing of reproduction is one of the important life history traits that influence fitness. Some organisms use photoperiods to reproduce during favorable seasons, but others lose such photoperiodism. Compared to the theoretical studies, we know little about the molecular genetic mechanisms underlying variations in the timing of reproduction. To answer these questions, we use the threespine stickleback fish as a model. Ancestral marine ecotypes generally show seasonal reproduction, whereas derived freshwater ecotypes exhibit great diversity in both the timing and duration of reproduction. Transcriptome analysis showed that TSHb2 exhibits photoperiodic change in marine ecotypes, but not in multiple freshwater populations. TALEN-induced knockdown experiments demonstrated that TSHb2 has a pleiotropic role in suppressing gonadotropic hormone expression, sex steroid hormone secretion, gonad development and body growth under short days, which may underlie their long and early reproduction.

Academic Significance and Societal Importance of the Research Achievements

甲状腺刺激ホルモンは、イトヨ以外の魚や鳥、哺乳類でも季節性繁殖の制御をおこなっていることが知られてきています。このため、イトヨ以外の他の生物でも、季節性繁殖の進化の鍵を担っていることが考えられます。この遺伝子を制御することにより、より長い期間繁殖する家畜種や養殖種の作成なども可能かもしれません。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (34 results)

All 2019 2018 2017 2016

All Journal Article (12 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 12 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (22 results) (of which Int'l Joint Research: 8 results,  Invited: 14 results)

  • [Journal Article] A key metabolic gene for recurrent freshwater colonization and radiation in fishes2019

    • Author(s)
      Asano Ishikawa et al.
    • Journal Title

      Science

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phylogenomics reveals habitat-associated body shape divergence in Oryzias woworae species group (Teleostei: Adrianichthyidae)2018

    • Author(s)
      Mokodongan DF, Montenegro J, Mochida K, Fujimoto S, Ishikawa A, Kakioka R, Yong L, Mulis, Hadiaty RK, Mandagi IF, Masengi WA, Wachi N, Hashiguchi Y, Kitano J, Yamahira K
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: 118 Pages: 194-203

    • DOI

      10.1016/j.ympev.2017.10.005

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sex Differences in Recombination in Sticklebacks2018

    • Author(s)
      Sardell Jason M.、Cheng Changde、Dagilis Andrius J.、Ishikawa Asano、Kitano Jun、Peichel Catherine L.、Kirkpatrick Mark
    • Journal Title

      G3:Genes|Genomes|Genetics

      Volume: 8 Issue: 6 Pages: 1971-1983

    • DOI

      10.1534/g3.118.200166

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lateralized expression of left-right axis formation genes is shared by adult brains of lefty and righty scale-eating cichlids2018

    • Author(s)
      Takeuchi Yuichi、Ishikawa Asano、Oda Yoichi、Kitano Jun
    • Journal Title

      Comparative Biochemistry and Physiology Part D: Genomics and Proteomics

      Volume: 28 Pages: 99-106

    • DOI

      10.1016/j.cbd.2018.07.002

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional divergence of a heterochromatin‐binding protein during stickleback speciation2018

    • Author(s)
      Yoshida Kohta、Ishikawa Asano、Toyoda Atsushi、Shigenobu Shuji、Fujiyama Asao、Kitano Jun
    • Journal Title

      Molecular Ecology

      Volume: 28 Issue: 6 Pages: 1563-1578

    • DOI

      10.1111/mec.14841

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parallel transcriptome evolution in stream threespine sticklebacks2018

    • Author(s)
      Kitano Jun、Ishikawa Asano、Kusakabe Makoto
    • Journal Title

      Development, Growth & Differentiation

      Volume: 61 Issue: 1 Pages: 104-113

    • DOI

      10.1111/dgd.12576

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex differences in recombination in sticklebacks.2018

    • Author(s)
      Sardell JM, Cheng C, Dagilis AJ, Ishikawa A, Kitano J, Peichel CL, and Kirkpatrick M.
    • Journal Title

      G3: Genes, Genomes, Genetics.

      Volume: in press

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Different contributions of local- and distant-regulatory changes to transcriptome divergence between stickleback ecotypes.2017

    • Author(s)
      Ishikawa A, Kusakabe M, Yoshida K, Ravinet M, MakinoT, Toyoda A, Fujiyama A, Kitano J.
    • Journal Title

      Evolution

      Volume: 71 Issue: 3 Pages: 565-581

    • DOI

      10.1111/evo.13175

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genetic basis for variation in salinity tolerance between stickleback ecotypes2017

    • Author(s)
      Kusakabe M, Ishikawa A, Ravinet M, Yoshida K, Makino T, Toyoda A, Fujiyama A, Kitano J
    • Journal Title

      Molecular Ecology

      Volume: 26 Issue: 1 Pages: 304-319

    • DOI

      10.1111/mec.13875

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] New localities of the Oryzias woworae species group (Adrianichthyidae) in Sulawesi Tenggara.2016

    • Author(s)
      Yamahira K, Mochida K, Fujimoto S, Mokodongan DF, Montenegro Gonzalez JA, Kaito T, Ishikawa A, Kitano J, Sue T, Mulis Hadiaty RK, Mandagi IF, Masengi AKW.
    • Journal Title

      Indonesian Journal of Ichthyology

      Volume: 16 Pages: 125-131

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparison of freshwater tolerance during spawning migration between two sympatric Japanese marine threespine stickleback species.2016

    • Author(s)
      Ishikawa A, Kusakabe M, Kume M, and Kitano J.
    • Journal Title

      Evolutionary Ecology Research

      Volume: 17 Pages: 525-534

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Trophic niche differentiation and phenotypic divergence among cryptic species of Japanese ninespine stickleback.2016

    • Author(s)
      Ravinet M, Ishikawa A, and Kitano J.
    • Journal Title

      Evolutionary Ecology Research

      Volume: 17 Pages: 505-523

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The molecular genetic mechanisms of life history evolution in sticklebacks.2019

    • Author(s)
      Ishikawa A. and Kitano J.
    • Organizer
      JEB2019: Genome editing for comparative physiology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] トゲウオから探る適応進化の分子遺伝機構2019

    • Author(s)
      石川 麻乃
    • Organizer
      岡山大学昆虫学土曜セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] イトヨにおける季節性生活史の進化とその分子遺伝基盤2019

    • Author(s)
      石川 麻乃
    • Organizer
      第66回日本生態学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] A key metabolic gene for stickleback freshwater colonization.2018

    • Author(s)
      Ishikawa A, and Kitano J.
    • Organizer
      Stickleback 2018, Nineth International Conference on Stickleback Behavior and Evolution
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic mechanism underlying speciation and adaptation in stickleback2018

    • Author(s)
      Ishikawa A
    • Organizer
      14th JGFoS symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular and genetic basis underlying freshwater colonization and adaptation in sticklebacks2018

    • Author(s)
      Ishikawa A.
    • Organizer
      The 10th NIBB International practical course
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] イトヨにおける季節性繁殖の収斂的進化とその分子遺伝基盤2018

    • Author(s)
      石川麻乃
    • Organizer
      第20回日本進化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] DHA合成能が担う新規環境への適応放散の遺伝基盤2018

    • Author(s)
      石川 麻乃
    • Organizer
      京大生態研セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] A metabolic key innovation enabled freshwater colonization and radiation by marine stickleback fish2018

    • Author(s)
      Ishikawa A
    • Organizer
      首都大バイオコンファレンス
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] イトヨの回遊パターンの多様性を生む遺伝基盤2018

    • Author(s)
      石川 麻乃
    • Organizer
      生物の移動研究集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular and genetic basis underlying freshwater colonization and adaptation in sticklebacks2018

    • Author(s)
      Ishikawa A, and Kitano J
    • Organizer
      The 1st AsiaEvo conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] DHA合成能が担う淡水域への適応放散とその遺伝基盤2017

    • Author(s)
      石川 麻乃
    • Organizer
      第64回日本生態学会
    • Place of Presentation
      東京
    • Year and Date
      2017-03-14
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Genetic mechanisms underlying variation in seasonal reproduction in sticklebacks.2017

    • Author(s)
      Ishikawa A, and Kitano J
    • Organizer
      Congress of the European Society of Evolutionary Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] DHA合成能が担うトゲウオの淡水進出とその遺伝基盤2017

    • Author(s)
      石川 麻乃
    • Organizer
      道東森里海連関シンポジウム2017
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] A key role for an omega-3 fatty acid desaturase gene in stickleback freshwater colonization2017

    • Author(s)
      Ishikawa A
    • Organizer
      第33回個体群生態学会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] DHA合成能が担う新規環境への適応放散の遺伝基盤2017

    • Author(s)
      石川 麻乃、北野潤
    • Organizer
      第88回日本動物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Non-parallel genetic mechanisms underlying parallel loss of seasonal photoperiodism in freshwater sticklebacks.2016

    • Author(s)
      Ishikawa A, and Kitano J
    • Organizer
      The 22nd International Congress of Zoology
    • Place of Presentation
      Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] DHA合成能が担う新規環境への適応放散の遺伝学2016

    • Author(s)
      石川 麻乃
    • Organizer
      日本遺伝学会第88回大会
    • Place of Presentation
      三島
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] トゲウオ科魚類イトヨにおける季節性繁殖の多様性とその遺伝基盤2016

    • Author(s)
      石川 麻乃
    • Organizer
      生物リズム若手研究者の集い2016
    • Place of Presentation
      山口
    • Year and Date
      2016-09-02
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] トランスクリプトーム進化の遺伝基盤とその制約2016

    • Author(s)
      石川 麻乃、日下部 誠、吉田 恒太、Mark Ravinet、牧野 能士、豊田 敦、藤山 秋佐夫、北野 潤
    • Organizer
      第18回日本進化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
  • [Presentation] DHAが担う新規環境への適応放散の遺伝機構2016

    • Author(s)
      石川 麻乃、北野 潤
    • Organizer
      第2回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
    • Place of Presentation
      東岡崎
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Molecular and genetic mechanisms underlying variation in seasonal reproduction between stickleback ecotypes2016

    • Author(s)
      石川 麻乃、北野 潤
    • Organizer
      第22回小型魚類研究会
    • Place of Presentation
      東岡崎
    • Year and Date
      2016-08-20
    • Related Report
      2016 Research-status Report
    • Invited

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Published: 2016-04-21   Modified: 2020-03-30  

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