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Experimental analysis of the evolution of symbiosis using an artificial ecosystem

Research Project

Project/Area Number 16K07520
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ecology/Environment
Research InstitutionEhime University

Principal Investigator

Toshiyuki Nakajima  愛媛大学, 理工学研究科(理学系), 教授 (70314945)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords共生の進化 / 実験進化 / 藻類 / 大腸菌 / 原生動物 / 共生 / 生態系 / ゲノム / 全ゲノム / 適応放散 / 進化 / マイクロコズム / 細菌 / 全ゲノム解析 / 微生物
Outline of Final Research Achievements

Living systems evolve in ecosystems, not in isolation. Before the present research, we synthesized an experimental ecosystem in a flask using three microbial species (an alga, E. coli, a protozoan), and cultivated them for ca. 13 years, in order to understand a possible mechanism of the evolution of component species under the restriction of matter/energy flow in an ecosystem. A previous research suggested that a primary stage of symbiotic associations between the alga and E. coli, and between the alga and the protozoan evolved in this long-term culture. In the present study, using algal and E. coli isolates obtained from 6, 8, 12-year cultures, we investigated their physiological and morphological traits, ecological interactions between them, and the whole-genome DNA sequences of E. coli isolates. The results indicated that the two species evolved some symbiotic associations, and that a variety of different genetic types coexisted in the experimental ecosystem.

Academic Significance and Societal Importance of the Research Achievements

微生物を用いて実験個体群や群集を作り,それを長期に培養し進化過程を明らかにする手法(実験進化)がある.しかし,従来の方法は着目する個体群のみを培養系に入れ,外部から資源を供給する形で行われてきた.本研究では,光照射のみで構成種が維持される自律型のモデル生態系を独自に開発した.これは,生態系から生物種を切り出さずに,生態系における生物進化を解析する全く新しい手法である.この培養系を13年間という長期に培養し,共生の進化の共存の仕組みを明らかにした.自然生態系は生物が互いに相互作用して進化した結果できた系あることから,本成果は,生物の共存の仕組みを進化の視点から理解することに大きく寄与する.

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2020 2019 2018 2017 2016 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Unification of Epistemic and Ontic Concepts of Information, Probability, and Entropy, Using Cognizers-System Model2019

    • Author(s)
      Toshiyuki Nakajima
    • Journal Title

      Entropy

      Volume: - Issue: 2 Pages: 1-26

    • DOI

      10.3390/e21020216

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ecological extension of the theory of evolution by natural selection from a perspective of Western and Eastern holistic philosophy2017

    • Author(s)
      Toshiyuki Nakajima
    • Journal Title

      Progress in Biophysics and Molecular Biology

      Volume: 131 Pages: 298-311

    • DOI

      10.1016/j.pbiomolbio.2017.09.005

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 合成生態系を用いた藻類-細菌の集塊形成による共生の進化2020

    • Author(s)
      安部雄一,藤井陽介、堀澤栄,佐久間洋,中島敏幸
    • Organizer
      日本ゲノム微生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 緑藻・大腸菌・テトラヒメナの3種からなる実験生態系の長期培養における緑藻-テトラヒメナの細胞内共生の進化と生態系過程2019

    • Author(s)
      中島敏幸,佐野明子,松原俊行,藤川佳之,大西陽一郎,松田達也
    • Organizer
      共生起源研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 合成生態系における藻類・細菌間の種間関係の進化と大腸菌の全ゲノム解析2019

    • Author(s)
      安部雄一,坪井睦枝,松浦正幸,藤川佳之,松本沙千,堀澤栄,佐久間洋, 中島敏幸
    • Organizer
      日本ゲノム微生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 藻類・細菌・繊毛虫からなる合成生態系を用いた進化の解析:大腸菌の進化と種間関係2019

    • Author(s)
      中島敏幸,安部雄一,坪井睦枝,松浦正幸,松本沙千,堀澤栄,佐久間洋
    • Organizer
      日本ゲノム微生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 長期培養モデル生態系における藻類・細菌間共生の進化2018

    • Author(s)
      安部雄一,坪井睦枝,松浦正幸,松本沙千,堀澤栄,佐久間洋,中島敏幸
    • Organizer
      日本原生生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 緑藻-繊毛虫の細胞内共生の進化における共生系内の資源交換と系外から取り込む資源の変化:3種モデル生態系を用いた解析2018

    • Author(s)
      松田達也,佐古壮一郎,西窪健太,中島敏幸
    • Organizer
      日本原生生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 実験培養系における藻類-繊毛虫細胞内共生の進化とこの共生系の安定に必要な系外の資源2016

    • Author(s)
      西窪健太、中島敏幸
    • Organizer
      日本原生生物学会
    • Place of Presentation
      岡山大学 (岡山県岡山市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] ダーウィンモデルの限界を超えて:適応進化の包括的モデルの展望2016

    • Author(s)
      中島敏幸
    • Organizer
      生物学基礎論研究会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県横浜市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 藻類・細菌・繊毛虫からなる人工生態系における細菌の適応放散と生存/繁殖戦略2016

    • Author(s)
      中島敏幸,藤井陽介,松浦正幸,松本沙
    • Organizer
      日本進化学会
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
  • [Presentation] volution of Symbiotic Associations in a Synthetic Microbial Ecosystem2016

    • Author(s)
      Toshiyuki Nakajima
    • Organizer
      American Society for Microbiology
    • Place of Presentation
      Boston, USA
    • Year and Date
      2016-06-18
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 遺伝子・多様性・循環の科学: 生態学の領域融合へ 10章:生態系とダーウィンマシーン2019

    • Author(s)
      中島敏幸 (門脇・立木編)
    • Total Pages
      432
    • Publisher
      京都大学学術出版会
    • ISBN
      4814001908
    • Related Report
      2018 Research-status Report
  • [Remarks] マイクロコズムの長期培養実験 から探る進化

    • URL

      https://www.sgmj.org/index.php?page=newsletter_index

    • Related Report
      2019 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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