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Evaluation of "evrything is everywhere" hypothesis using mercury resistance bacteria as tracer microorganism

Research Project

Project/Area Number 16K07529
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ecology/Environment
Research InstitutionKindai University

Principal Investigator

MATSUI Kazuaki  近畿大学, 理工学部, 教授 (40435532)

Research Collaborator SILVER simon  
ENDO ginro  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords水銀耐性 / Bacilli / 微生物の分散 / 遺伝子水平伝播 / 水銀耐性細菌 / 環境による選択 / merA / 細菌の分散 / everything is everywhere / 水銀耐性トランスポゾン / 遺伝子伝播 / 微生物生態
Outline of Final Research Achievements

In microbial ecosystems, the hypotheses of environmental selection and dispersal limitation are the most likely explanations for how new ecosystems form. However, the interactions between these two phenomena are not well characterized. To test the “everything is everywhere but the environments select” hypothesis, mercury resistant spore-forming Bacilli were isolated from different geographical regions in order to understand the dissemination of mercury resistance transposons across related Bacilli genera. Tn5084, originally identified from B. cereus strain RC607 from the USA, is identified from Bacillus isolated from Asia and Europe. Newly identified transposons Tn6294 and TndMER3 were also identified in soils from different continents. The Soil cultivation with mercury containing media leads to the enhancement of mer genes. The results obtained here indicated that the horizontal dissemination of mercury resistance transposons in the Bacilli.

Academic Significance and Societal Importance of the Research Achievements

大型の生物と違って、肉眼での観察が難しい微生物の移出入や新しい環境への定着機構については不明な点が多い。そこでバチルス属水銀耐性細菌を指標に細菌や遺伝子の動態を解析することで、これまで仮説に留まっていた微生物の移動実態を実証することを試みた。微生物の環境中の動態を明らかにすることは、微生物の動態に関わる様々な課題(薬剤耐性細菌の環境中での動態、病原性微生物の拡散、下水越流が河川水に及ぼす衛生面)に科学的に対応する上での重要な知見となる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] University of Illinois at Chicago(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Illinois at Chicago(U.S.A.)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] University of Illinois at Chicago(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Mercury bioremediation by mercury resistance transposon-mediated in situ molecular breeding2018

    • Author(s)
      Matsui Kazuaki、Endo Ginro
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 102 Issue: 7 Pages: 3037-3048

    • DOI

      10.1007/s00253-018-8847-2

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mercury resistance transposons in Bacilli strains from different geographical regions2016

    • Author(s)
      Kazuaki Matsui, Satoshi Yoshinami, Masaru Narita, Mei-Fang Chien, Le T. Phung, Simon Silver, Ginro Endo
    • Journal Title

      FEMS Microbiology Letters

      Volume: 363 Issue: 5 Pages: fnw013-fnw013

    • DOI

      10.1093/femsle/fnw013

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Real-time PCR assay for evaluating the distribution of mercury-resistant Bacilli in environmental soils.2018

    • Author(s)
      Kazuaki Matsui, Ginro Endo
    • Organizer
      ASM Microbe 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水銀Bacilliの分布および存在量の把握に向けた定量PCR法の検討2017

    • Author(s)
      松井一彰、鈴木孝司、簡梅芳、遠藤銀朗
    • Organizer
      環境微生物系学会合同大会 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Dissemination of mercury resistance transposons among Bacilli across geographical barriers.2016

    • Author(s)
      Matsui, K., S. Yoshinami, M. Narita, M-F. Chien, LT. Phung, S. Silver and G. Endo.
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2016-08-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2022-02-16  

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