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Development of new breeding material using dominant mutants causing by insertions of DNA transposon, nDart1

Research Project

Project/Area Number 16K07561
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Tsugane Kazuo  基礎生物学研究所, 多様性生物学研究室, 助教 (50343744)

Co-Investigator(Kenkyū-buntansha) 前川 雅彦  岡山大学, 資源植物科学研究所, 教授 (00142703)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsイネ / 優性変異 / 顕性変異 / 大粒 / トランスポゾン / 優性 / 顕性 / 転移因子 / 優性(顕性) / 育種素材 / 変異創生
Outline of Final Research Achievements

Transposons occupying a large portion of the rice genome. Active DNA transposons are important tools for gene functional analysis. The endogenous non-autonomous transposon, nDart1, in rice is said to generate various transposon-insertion mutants because nDart1 elements tend to insert into genic regions under natural growth conditions.
The Lgg mutant which was isolated from nDart1-promoted mutant plants bore slightly large grains as a dominant inheritance. Transposon-display identified the insertion site of nDart1 in the Lgg mutant. Identified LGG genes show the similarity with RNA binding proteins. The expression of LGG gene in Lgg mutant was lower than WT. Lgg mutation was essentially caused by the insertion of nDart1, knock-out mutant using genome editing (GE) and over-expressing (OE) lines were generated. GE lines showed large grain phenotypes that were the same as Lgg mutants. On the contrary, harvested seeds from the OE line were smaller than the WT and GE lines.

Academic Significance and Societal Importance of the Research Achievements

申請者はこれまでに様々なnDart1挿入変異体を解析して原因遺伝子の機能を解明してきた。特に近年は顕性変異に注目し、トランスポゾンの挿入によってしか得られない表現型の解析に力を注いでいる。顕性変異は交配によって直ぐに表現型を確認できたり、倍数性の高い植物でも表現型が現れ易いので利用し易い面がある。日本だけでなく多くの国々では遺伝子組換え植物に対して、十分なパブリックアクセプタンスを得られていない。遺伝子組換え体ではないKoshiタグラインの利用や理解を社会に広めることによって、遺伝子組換え技術に対するリテラシーをあげていくことにも貢献できると考えている。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Establishment of <i>nDart1</i>-tagged lines of Koshihikari, an elite variety of rice in Japan2019

    • Author(s)
      Nishimura Hideki、Himi Eiko、Rikiishi Kazuhide、Tsugane Kazuo、Maekawa Masahiko
    • Journal Title

      Breeding Science

      Volume: 69 Issue: 4 Pages: 696-701

    • DOI

      10.1270/jsbbs.19049

    • NAID

      130007772532

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transgenerational activation of an autonomous DNA transposon, Dart1-24, by 5-azaC treatment in rice2019

    • Author(s)
      2.Nishimura H, Himi E, Eun CH, Takahashi H, Qian Q, *Tsugane K, Maekawa M.
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 132 Issue: 12 Pages: 3347-3355

    • DOI

      10.1007/s00122-019-03429-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] イネのトランスポゾンを用いた優性変異を利用した育種素材の開発2019

    • Author(s)
      栂根 一夫、前川 雅彦
    • Journal Title

      アグリバイオ

      Volume: 3 Pages: 87-91

    • NAID

      40022053372

    • Related Report
      2019 Annual Research Report
  • [Journal Article] LARGE GRAIN Encodes a Putative RNA-Binding Protein that Regulates Spikelet Hull Length in Rice2019

    • Author(s)
      Chiou Wan-Yi、Kawamoto Tadafumi、Himi Eiko、Rikiishi Kazuhide、Sugimoto Manabu、Hayashi-Tsugane Mika、Tsugane Kazuo、Maekawa Masahiko
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 3 Pages: 503-515

    • DOI

      10.1093/pcp/pcz014

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Easy sectioning of whole grain of rice using cryomicrotome2018

    • Author(s)
      Chiou Wan-Yi、Tsugane Kazuo、Kawamoto Tadafumi、Maekawa Masahiko
    • Journal Title

      Breeding Science

      Volume: 68 Issue: 3 Pages: 381-384

    • DOI

      10.1270/jsbbs.17103

    • NAID

      130007430193

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Easy sectioning of whole grain of rice using cryomicrotome2018

    • Author(s)
      Wanyi Chiou, Kazuo Tsugane, Tadafumi Kawamoto, Masahiko Maekawa
    • Journal Title

      Breeding Science

      Volume: 印刷中 Pages: 1-1

    • NAID

      130007430193

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Morphological Characterization of Spikelet and Microarray Analysis of the Large grain Mutant found in Transposon‐tagged Lines in Rice (Oryza sativa L.)2018

    • Author(s)
      Chiou Wan-Yi, 川本忠文, 力石和英, 氷見英子, 西村秀希, 栂根一夫, 前川雅彦
    • Organizer
      日本育種学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Characterization of Spikelet and Microarray Analysis of the Large grain Mutant found in Transposon‐tagged Lines in Rice (Oryza sativa L.)2018

    • Author(s)
      Chiou Wanyi, 川本忠文, 力石和英, 氷見英子, 西村秀希, 栂根一夫, 前川雅彦
    • Organizer
      日本育種学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Genetic analysis of the large grain mutant found in transposon-tagged lines of rice2017

    • Author(s)
      Chiou Wayni, Kazuhide Rikiishi, Eiko Himi, Hideki Nishimura, Kazuo Tsugane, Masahiko Maekawa
    • Organizer
      日本植物生理学会・台湾植物学会(TSPB)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] DNAトランスポゾンnDart1の遺伝子への挿入によって生じるイネの優性変異の解析2016

    • Author(s)
      栂根一夫、栂根美佳、前川政彦
    • Organizer
      日本遺伝学会88回大会
    • Place of Presentation
      静岡県三島市 日本大学国際関係学部
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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