Analysis of physiological mechanisms and quantitative trait loci for salinity tolerance related to grain productivity in rice

• Project/Area Number: 16K07573
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Crop production science
• Research Institution: Okayama University
• Principal Investigator: Hirai Yoshihiko 岡山大学, 環境生命科学研究科, 准教授 (80263622)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 耐塩性 / イネ / 量的形質遺伝子座 / 収量 / QTL

Outline of Final Research Achievements

Comparison of plant growth and grain yield using progeny populations and lines derived from a cross between Koshihikari and Nona Bokra (or IR64) grown under long-term saline conditions suggests that the salinity tolerance in several lines were related to Na+ and Cl- exclusion. Four salt-tolerance QTLs located on chromosome 2 related to grain yield, panicle number, and panicle dry weight were detected by using F2 plants from a cross between Koshihikari and SL506 (or SL2007). Furthermore, it was suggested that plant dry weight and grain yield under long-term salinity conditions was reduced by Cl－ toxicity rather than Na+ toxicity.

Academic Significance and Societal Importance of the Research Achievements

本研究おいて，塩条件下での収量維持にはCl-害が密接に関わることが示唆されたことは，耐塩性に重要な生理的要因を示唆したものであり，学術的意義が高い．また，収量形質に関わる耐塩性QTLが染色体上の複数領域に見いだされた．このことは，耐塩性機構の解析と遺伝子同定に有用な系統の育成につながることから有意義である．さらに，QTL 近傍マーカーの情報は，耐塩性育種にそのまま用いることが可能であり，その点においても意義が高いと考えられる．

Research Products

• [Journal Article] Identification and Characterization of Chromosome Regions Associated With Salinity Tolerance in Rice (2018)
  • Author(s): Thuy Nguyen Thi Thu、Tokuyasu Misato、Mai Nguyen Sao、Hirai Yoshihiko
  • Journal Title: Journal of Agricultural Science Volume: 10 Issue: 11 Pages: 57-57
  • DOI: 10.5539/jas.v10n11p57
  • Peer Reviewed / Open Access
• [Journal Article] Cl- More Detrimental Than Na+ in Rice Under Long-Term Saline Conditions (2018)
  • Author(s): Dao Hanh Duy、Hirai Yoshihiko
  • Journal Title: Journal of Agricultural Science Volume: 10 Issue: 10 Pages: 66-66
  • DOI: 10.5539/jas.v10n10p66
  • Peer Reviewed / Open Access
• [Presentation] QTL analysis for salt tolerance in rice under long-term salt stress using Koshihikari/IR64 chromosome segment substitution lines and backcross progeny F2 population (2019)
  • Author(s): Mai Nguyen Sao, Mai Fukada, Hanh Duy Dao and Yoshihiko Hirai
  • Organizer: 日本作物学会第247回講演会
• [Presentation] Identification and characterization of chromosome regions associated with salt tolerance in rice under long-term salt stress using chromosome segment substitution lines derived from Koshihikari and Nona Bokra (2017)
  • Author(s): Thuy Nguyen Thi Thu, Misato Tokuyasu, Makoto Tsuda and Yoshihiko Hirai
  • Organizer: 日本作物学会第243回講演会
  • Place of Presentation: 東京大学
  • Year and Date: 2017-03-29