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Analysis of nitrogen dynamics in rice plants.

Research Project

Project/Area Number 16K07578
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Crop production science
Research InstitutionIshikawa Prefectural University

Principal Investigator

Tsukaguchi Tadashi  石川県立大学, 生物資源環境学部, 准教授 (40345492)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsイネ / 窒素利用効率 / タンパク質含量 / 子実窒素集積性 / 窒素分配 / 子実タンパク質集積性 / QTL / 水稲 / 窒素動態 / 量的形質遺伝子座(QTL) / 玄米タンパク質濃度 / 登熟 / QTL解析 / 準同質遺伝子系統
Outline of Final Research Achievements

We hypothesize that nitrogen allocation to rice grains is mostly determined by the grain-protein-accumulation ability which is genetically determined and is not affected by environments and aim to clarify the grain-protein-accumulation ability genetically and physio-ecologically. We developed a quantitative measure for the evaluation of grain-protein-accumulation ability, compared it among rice cultivars and selected two cultivars, one with high and one with low grain-protein-accumulation ability. Using these cultivars as parents we developed F2 and F3 populations. QTL (quantitative trait loci) analysis with these populations revealed that several QTLs contributed grain-protein-accumulation ability. Among these QTLs, two QTLs were detected in both generations and both nitrogen conditions. We now are developing near isogenic lines with the introgression of chromosome segments of the other parent’s genotype at these two QTL regions to validate the effect of these QTLs.

Academic Significance and Societal Importance of the Research Achievements

本研究により、子実窒素集積性は他の遺伝形質や環境要因ともに、子実のタンパク質含量、登熟期のイネ体窒素動態に寄与することが分かった。イネの子実タンパク質含量は栄養的および食味的な側面から重要な形質である。また、持続的な農業生産や環境負荷を最低限にしながら収量性を高めることが重要でなり、イネ体の窒素利用効率の高い品種の育成が求められるが、そのためにはイネ体窒素動態の解明が不可欠でかる。本研究の成果はそれらを目的とした品種育成に寄与することが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] 夜間掛流し灌漑が出穂後20日間の平均水温・地温および米の外観品質に与える影響.2018

    • Author(s)
      西田和弘・塚口直史・二宮悠樹・宇尾卓也・吉田修一郎・塩沢昌
    • Journal Title

      農業農村工学会論文集.

      Volume: 306 Pages: 105-115

    • NAID

      220000164647

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of high temperature and shading on grain abscisic acid content and grain filling pattern in rice (Oryza sativa L.).2018

    • Author(s)
      Tsukaguchi, T., Tanaka, R., Inoue, H. and Nakagawa, H.
    • Journal Title

      Plant Production Science

      Volume: 21 Issue: 4 Pages: 333-339

    • DOI

      10.1080/1343943x.2018.1524264

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 子実窒素集積に着目したイネ体窒素分配の解析.2018

    • Author(s)
      塚口直史
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 76-80

    • NAID

      40021803271

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 夜間掛流し灌漑が出穂後20日間の平均水温・地温および米の外観品質に与える影響2018

    • Author(s)
      西田和弘・塚口直史・二宮悠樹・宇尾卓也・吉田修一郎・塩沢昌
    • Journal Title

      農業農村工学会論文集

      Volume: 印刷中

    • NAID

      220000164647

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Detection and validation of QTLs for milky-white grains caused by high temperature during the ripening period in <i>Japonica</i> rice2017

    • Author(s)
      Miyahara, K., Wada, T., Sonoda, J., Tsukaguchi, T., Miyazaki, M., Tsubone, M., Yamaguchi, O., Ishibashi, M., Iwasawa, N., Umemoto, T., Kondo, M.
    • Journal Title

      Breeding Science

      Volume: 67 Issue: 4 Pages: 333-339

    • DOI

      10.1270/jsbbs.16203

    • NAID

      130006164471

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cultivar differences in the grain protein accumulation ability in rice (Oryza sativa L.)2016

    • Author(s)
      Tsukaguchi, T., Nitta, S., Matsuno, Y.
    • Journal Title

      Field Crops Research

      Volume: 192 Pages: 110-117

    • DOI

      10.1016/j.fcr.2016.04.022

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The effects of nitrogen uptake before and after heading on grain protein content and the occurrence of basal- and back-white grains in rice (Oryza sativa L.).2016

    • Author(s)
      Tsukaguchi, T., Taniguchi, Y., Ito, R.
    • Journal Title

      Plant Production Science.

      Volume: 19 Issue: 4 Pages: 508-517

    • DOI

      10.1080/1343943x.2016.1223527

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A quantitative measure for assimilate partitioning efficiency in rice (Oryza sativa L.).2016

    • Author(s)
      Tsukaguchi, T., Murakami, K., Michimoto, T.
    • Journal Title

      Field Crops Research

      Volume: 198 Pages: 122-130

    • DOI

      10.1016/j.fcr.2016.08.033

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 水稲の高温障害抑制のための掛流し灌漑が水田の窒素環境と稲の窒素吸収に与える影響.2018

    • Author(s)
      西田和弘・柴田里子・塚口直史・吉田修一郎・塩沢昌
    • Organizer
      平成30年度農業農村工学会大会講演会(京都)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 水稲の高温障害抑制のための掛流し灌漑が田面水・土壌水の窒素濃度と玄米タンパク質濃度に与える影響.2018

    • Author(s)
      西田和弘・柴田里子・塚口直史・吉田修一郎・塩沢昌
    • Organizer
      日本土壌肥料学会2018年度神奈川大会(藤沢)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Effect of continuous irrigation with running water on the nitrogen environment in a paddy field.2018

    • Author(s)
      Nishida, K., Shibata, S., Tsukaguchi, T., Yoshida, Y., and Shiozawa, S.
    • Organizer
      PAWEES & INWEPF international conference 2018 (Nara)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ダイズの栽植密度が収量および品質に及ぼす影響.2018

    • Author(s)
      丹保彩香・今本裕士・吉田翔伍・塚口直史
    • Organizer
      北陸作物・育種学会第55回講演会(長岡)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 発育予測モデルによる水稲新品種「石川65号」の出穂予測.2018

    • Author(s)
      宇野史生・島田雅博・吉田ひろえ・中川博視・塚口直史
    • Organizer
      北陸作物・育種学会第55回講演会(長岡)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 窒素が決めるコメの品質.2018

    • Author(s)
      塚口直史
    • Organizer
      平成30年度石川県土壌肥料懇話会(金沢)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 石川県で育成された水稲新品種「石川65号」における品質を考慮した目標収量の設定.2017

    • Author(s)
      宇野史生・島田雅博・塚口直史
    • Organizer
      日本作物学会第243回講演会
    • Place of Presentation
      東京大学農学部
    • Year and Date
      2017-03-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 水稲の登熟期窒素動態の支配要因としての玄米タンパク質集積性.2016

    • Author(s)
      松野由莉・新田純代・塚口直史
    • Organizer
      日本作物学会第242回講演会
    • Place of Presentation
      龍谷大学瀬田キャンパス
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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