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Analysis of non-self recognition system of F-box gene for pollen in self-incompatibility using citrus doubled haploids.

Research Project

Project/Area Number 16K07599
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionUniversity of Miyazaki

Principal Investigator

KUNITAKE HISATO  宮崎大学, 農学部, 教授 (80289628)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsカンキツ / 自家不和合性 / 花粉 / SSR / 倍加半数体 / 非自己認識 / ハプロタイプ / 液体花粉培養 / 半数体 / 単為発生 / 花粉DNA / 雌性配偶子 / マイクロマニュピレーター
Outline of Final Research Achievements

Mechanism of the pollen S gene expression in flowering plants are difficult to interpret, because of the complex physiological phenomena that occur in the stigma and the style tissue. In this study, we examine about the non-self or self-recognition system using the mature pollen culture in self-incompatibility(SI) of citrus. As a result of investigating the multiple microsatellite (SSR) genotyping of a single pollen grain, for analyzing the element of pollen S gene and its segregation, it was revealed that seven of the 8 segregating loci in the pollen population of ‘Banpeiyu’ pummelo(Citrus maxima (Burm.)Merr.) fit a 1: 1 ratio. In addition, the results of inhibition rate of pollen tube using the mature pollen culture were showed the possibility having non-self recognition by multiple factors SI system.

Academic Significance and Societal Importance of the Research Achievements

主要なカンキツのひとつである‘晩白柚’ブンタンの花粉1粒におけるSSR解析技術が確立できたことは、自家不和合性因子の解析だけでなく、様々な有用遺伝子の解析を促進するものである。特に、液体花粉培養による自己・非自己認識機構の評価を行い、バラ科植物のように、複数の花粉側因子によって花柱S因子が認識される可能性が明らかになったことは大きな成果である。これらの成果を活かし、カンキツの自家不和合性の機序が明らかになり、化学的および物理的なストレス付与による自家和合性への転換機構が確立され、植物生長調節物質等の農薬応用が期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (4 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Characteristics in autotetraploid kumquats (Fortunella spp.) induced bycolchicine treatment to nucellar embryos and their utilization for triploid breeding.2019

    • Author(s)
      Tsunaki Nukayaa, Miki Sudo, Masaki Yahata, Yoshiyuki Nakajo, Tomohiro Ohta, Kiichi Yasuda, Akiyoshi Tominaga, Hiroo Mukai and Hisato Kunitake
    • Journal Title

      Scientia Horticulturae

      Volume: 245 Pages: 210-217

    • DOI

      10.1016/j.scienta.2018.10.015

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Morphological Characterization and Evaluation of Reproductive Function in a Haploid Pummelo [<i>Citrus maxima</i> (Burm.) Merr.]2017

    • Author(s)
      Masaki YAHATA, Hisato KUNITAKE, Haruki KOMATSU
    • Journal Title

      Japan Agricultural Research Quarterly: JARQ

      Volume: 51 Issue: 4 Pages: 293-298

    • DOI

      10.6090/jarq.51.293

    • NAID

      130006179795

    • ISSN
      0021-3551, 2185-8896
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Self-incompatible-like response induces alterations of actin cytoskeleton and cytosolic calcium in the Citrus pollen tube of ‘Banpeiyu’ and ‘Hyuganatsu’2016

    • Author(s)
      Li, Y., T. Fuse, S. Kawano and H. Kunitake
    • Journal Title

      Acta Hortic.

      Volume: 1135 Issue: 1135 Pages: 109-115

    • DOI

      10.17660/actahortic.2016.1135.13

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ブンタン倍加半数体の交雑後代におけるSSRマーカーによる遺伝子型解析2019

    • Author(s)
      河野美樹・西川奈菜・末吉乃梨子・八幡昌紀・清水徳朗・平野智也・國武久登
    • Organizer
      園芸学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カンキツにおける単一花粉のSSRマーカーによる遺伝子型解析2018

    • Author(s)
      河野美樹・西川奈菜・末吉乃梨子・八幡昌紀・清水徳朗・平野智也・國武久登
    • Organizer
      園芸学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 二倍体ブンタンと三倍体グレープフルーツの交雑から得られた種子の種類と倍数体との関係2017

    • Author(s)
      永嶋友香・國武久登・八幡昌紀(他6名)
    • Organizer
      園芸学会平成29年度春季大会
    • Place of Presentation
      日本大学生物資源科学部
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] SSRマーカーによる半数体ブンタンの遺伝子型解析2017

    • Author(s)
      西川奈菜・末吉乃梨子・八幡昌紀・清水徳朗・平野智也・國武久登
    • Organizer
      園芸学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Book] Citrus, Health Benefits and Production Technology2019

    • Author(s)
      Masaki Yahata and Hisato Kunitake
    • Total Pages
      10
    • Publisher
      IntechOpen
    • ISBN
      9781789854282
    • Related Report
      2018 Annual Research Report
  • [Remarks] 宮崎大学農学部植物遺伝育種学研究室ホームページ

    • URL

      http://www.agr.miyazaki-u.ac.jp/~abs/plant_breeding/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 宮崎大学農学部応用生物科学科植物遺伝育種学研究室ホームページ

    • URL

      http://www.agr.miyazaki-u.ac.jp/~abs/plant_breeding/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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