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Comparion of expression levels of CCD4 gene among azalea species belonging to subgenus tsutsusi and its application to breeding of yellow flowered evergreen azaleas

Research Project

Project/Area Number 16K07602
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionUniversity of the Ryukyus

Principal Investigator

URESHINO Kenji  琉球大学, 農学部, 教授 (10333759)

Co-Investigator(Kenkyū-buntansha) 宮島 郁夫  九州大学, 熱帯農学研究センター, 准教授 (20182024)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsツツジ / カロテノイド / CCD4 / サキシマツツジ / 黄色 / 黄色花
Outline of Final Research Achievements

Intersubgeneric crosses between white-flowered evergreen azalea species and Rhododendron japonicum f. flavum can produce yellow-flowered evergreen azaleas. The petal color of F1 progenies is known to fade during petal development, due to a high expression of the CCD4 gene inherited from the evergreen species. In this study, to facilitate the selection of evergreen azalea species as cross parents that have low expression levels of CCD4 in developing petals, CCD4 expression levels were compared among 16 evergreen azalea species. The expression levels of CCD4 genes in petals of evergreen azalea species at the day of anthesis varied widely from 2 to 20 times relative to that in R. japonicum. Among these species, R. amanoi maintained low CCD4 expression levels throughout all petal development stages, which were not significantly different from those of R. japonicum. This species, therefore, is considered a promising breeding material for producing yellow-flowered evergreen azaleas.

Academic Significance and Societal Importance of the Research Achievements

花卉類における黄花品種の作出は,多くの種目において育種目標としてあげられている.常緑性黄色花ツツジ作出を目的とした,白花常緑性ツツジと黄花落葉性キレンゲツツジとの交配において,F1実生は,カロテノイド色素生合成能自体は優性形質としてキレンゲツツジから遺伝しているものの,常緑性種より遺伝したCCD4の高発現により退色化している.本研究では,複数の常緑性ツツジ種について開花花弁におけるCCD4遺伝子発現量を調査し,発現量の低い常緑性種を明らかにしている.この結果は,今後,本種を交配親に用いることで,常緑性黄色花ツツジ作出の可能性を示しており,学術的意義がある.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017 2016

All Presentation (6 results)

  • [Presentation] 常緑性ツツジと落葉性キレンゲツツジとの亜属間交配で得られた実生の黄色花弁退色化要因の解明(第9報)数種の常緑性ツツジの花弁発育に伴うCCD4遺伝子の発現量の変化2019

    • Author(s)
      嬉野健次・宮島郁夫
    • Organizer
      園芸学会平成31年度春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 常緑性ツツジと落葉性キレンゲツツジとの亜属間交配で得られた実生の黄色花弁退色化要因の解明(第8報) ツツジ亜属内のCCD4遺伝子の相同性およびイントロンサイズの比較2018

    • Author(s)
      嬉野健次・伊禮凪沙
    • Organizer
      園芸学会平成30年度秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 常緑性ツツジと落葉性キレンゲツツジとの亜属間交配で得られた実生の黄色花弁退色化要因の解明(第7報)常緑性ツツジとキレンゲツツジ間のCCD4遺伝子ゲノム構造の比較2018

    • Author(s)
      嬉野健次、伊禮凪沙
    • Organizer
      園芸学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 常緑性ツツジと落葉性キレンゲツツジとの亜属間交配で得られた実生の黄色花弁退色化要因の解明(第6報)常緑性ツツジ種花弁内におけるCCD4遺伝子の発現量の比較2017

    • Author(s)
      嬉野健次、高良浩也
    • Organizer
      園芸学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 葉緑体ハプロタイプからみたタイワンヤマツツジの地理的変化2016

    • Author(s)
      嬉野健次,新垣友美,D.T.H. Huyen,宮島郁夫
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学 (愛知県名古屋市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] タイワンヤマツツジ花弁の赤紫色のブロッチ部にはクェルセチン3-グルコシドとクェルセチン3-ラムノシドが含まれる2016

    • Author(s)
      Huyen, DT.H.,D.T. Van,嬉野健次,宮島郁夫
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学 (愛知県名古屋市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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