Comparison of mutation frequency of the avirulence gene AVR-Pia introduced to different genomic region of Pyricularia oryzae.

• Project/Area Number: 16K07615
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine (2018); Kobe University (2016-2017)
• Principal Investigator: Chuma Izumi 帯広畜産大学, 畜産学部, 准教授 (90628926)
• Research Collaborator: Arazoe Takayuki 東京理科大学 ; Sone Teruo 北海道大学 ; Le Dinh Don ノンラム大学 ; Nguyen Thi Thin Nga 農業遺伝学研究所 ; Cumagun Christian フィリピン大学, ロスバニョス校 ; Sreewongchai Tanee カセサート大学 ; Spring Otmar ホーヘンハイム大学
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: イネ科植物いもち病 / いもち病菌 / 非病原力遺伝子 / 病原性変異機構 / イネ科植物いもち病菌 / イネいもち病菌 / AVR-Pizt / 病原性変異 / 転移因子 / Pyricularia oryzae

Outline of Final Research Achievements

We compared frequency of mutation of the avirurence gene AVR-Pia which was introduced to repeat rich (RR) and repeat poor (RP) regions of rice blast fungus, Pyricularia oryzae. Resistant rice cultivar Aichi-asahi (Pia) were inoculated with transformants ta#126 which has AVR-Pia in RR region and zt#1 which has AVR-Pia in RP region. When compared mutants isolated from susceptible lesions of inoculated rice leaves, mutation frequency of ta#126 was higher than that of zt#1. Mutation mechanism of all mutants were deletion of introduced AVR-Pia. The deleted region were surrounded by transposable elements and its size depends on the distance between transposable elements.

Academic Significance and Societal Importance of the Research Achievements

世界のイネの最重要病原体のひとつであるイネいもち病菌は、イネ品種に対する病原性変異が多様であり、この変異メカニズムの解明と変異リスクの予測は、抵抗性育種への応用にも繋がる。いもち病菌が宿主に対する病原性の決定を担っているのが非病原力遺伝子であるが、これらの遺伝子の重要な変異機構としてゲノムからの欠失が挙げられる。本研究結果は、非病原力遺伝子が欠失するか否かを決定するのが周辺に存在する転移因子であり、転移因子頻度の高いゲノム領域に存在する非病原力遺伝子は欠失するリスクの高い状態にあることを示唆するものである。

Research Products

• [Int'l Joint Research] University of Phillopines Los Bagnios(フィリピン)
• [Int'l Joint Research] Nong Lam University/Agricultural Genetics Institute/Plant Protection Research Institute(ベトナム)
• [Int'l Joint Research] Kasetsart University(タイ)
• [Int'l Joint Research] University of Philippines Los Bagnios/IRRI(フィリピン)
• [Int'l Joint Research] Nong Lam University/Agricultural Genetics Institute/Plant Protecthion Research Institute(ベトナム)
• [Int'l Joint Research] Kasetsart University(Thailand)
• [Journal Article] Blast fungal genomes show frequent chromosomal changes, gene gains and losses, and effector gene turnover. (2019)
  • Author(s): Gomez Luciano LB, Jason Tsai I, Chuma I, Tosa Y, Chen YH, Li JY, Li MY, Jade Lu MY, Nakayashiki H, Li WH.
  • Journal Title: Mol. Biol, Evol. Volume: 36 Issue: 6 Pages: 1148-1161
  • DOI: 10.1093/molbev/msz045
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rmg8 and Rmg7, wheat genes for resistance to the wheat blastfungus, recognize the same avirulence gene AVR-Rmg8 (2018)
  • Author(s): Anh, V. Y. Inoue, S. Asuke, T.T.P. Vy, N.T. Anh, S. Wang, I. Chuma, Y. Tosa
  • Journal Title: Mol. Plant Pthology Volume: 19 Issue: 5 Pages: 1252-1256
  • DOI: 10.1111/mpp.12609
  • Peer Reviewed
• [Journal Article] A New Resistance Gene in Combination with Rmg8 Confers Strong Resistance Against Triticum Isolates of Pyricularia oryzae in a Common Wheat Landrace (2018)
  • Author(s): Wang Shizhen、Asuke Soichiro、Vy Trinh Thi Phuong、Inoue Yoshihiro、Chuma Izumi、Win Joe、Kato Kenji、Tosa Yukio
  • Journal Title: Phytopathology Volume: 108 Issue: 11 Pages: 1299-1306
  • DOI: 10.1094/phyto-12-17-0400-r
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A new resistance gene in combination with Rmg8 confers strong resistance against Triticum isolates of Pyricularia oryzae in a common wheat landrace. (2018)
  • Author(s): Wang, S., S. Asuke, T.T.P. Vy, Y. Inoue, I. Chuma, J. Win, K. Kato, Y. Tosa
  • Journal Title: Phytopathology Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First report of Fusarium solani species complex as a causal agent of Erythrina variegata decline and death after gall formation by Quadrastichus erythrinae on Okinawa Island, Japan. (2017)
  • Author(s): Kuroda, K., I. Chuma*, T. Kihara, T. Murakami, K. Takashina, D. Hiraoka, N. Kameyama.
  • Journal Title: Journal of General Plant Pathology Volume: 83 Pages: 344-357
  • Peer Reviewed / Open Access
• [Journal Article] Evolution of the wheat blast fungus through functional losses in a host specificity determinant. (2017)
  • Author(s): Inoue, Y., T.T.P. Vy, K. Yoshida, H. Asano, C. Mitsuoka, S Asuke, V.L. Anh, C.J. Cumagun, I. Chuma, R. Terauchi, K. Kato, T. Mitchell, B. Valent, M. Farman, Y. Tosa
  • Journal Title: Science Volume: 357 Pages: 80-83
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Host specialization of the blast fungus Magnaporthe oryzae is associated with dynamic gain and loss of genes linked to transposable elements. (2016)
  • Author(s): Yoshida K, Saunders DGO, Mitsuoka C, Natsume S, Kosugi S, Saitoh H, Inoue Y, Chuma I, Tosa Y, Cano LM, Kamoun S, Terauchi, R
  • Journal Title: BMC Genomics Volume: 17 Pages: 370-370
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Generic names in Magnaporthales. (2016)
  • Author(s): Zhang, N., J. Luo, A.Y. Rossman, T. Aoki, I. Chuma, P.W. Crous, R. Dean, R.P. de Vries, N. Donofrio, K.D. Hyde, M.-H. Lebrun, N.J. Talbot, D. Tharreau, Y. Tosa, B. Valent, Z. Wang, and J.-R. Xu.
  • Journal Title: IMA Fungus Volume: 7 Pages: 155-159
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genetic analysis of the resistance of barley to cryptic species of Pyricularia. (2016)
  • Author(s): Tagle, A.G., I. Chuma, H. Hisano, K. Sato, and Y. Tosa
  • Journal Title: Journal of General Plant Pathology Volume: 82 Pages: 302-306
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Anthracnose of black locust caused by Colletotrichum nymphaeae (Passerini) Aa. (2016)
  • Author(s): Yamagishi, N., T. Sato, I. Chuma, Y. Ishiyama, and Y. Tosa
  • Journal Title: Journal of General Plant Pathology Volume: 82 Pages: 174-176
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] イネいもち病菌非病原力遺伝子のrepeat-poor領域における欠失 (2018)
  • Author(s): 平岡大輝，荒添貴之，曾根輝雄，土佐幸雄，中馬いづみ
  • Organizer: 平成31年度日本植物病理学会大会
• [Presentation] Fusarium solani種複合体に属する菌のデイゴへの病原性と沖縄県内の地理的分布 (2018)
  • Author(s): 高階空也，中馬いづみ，亀山統一，黒田慶子
  • Organizer: 平成31年度日本植物病理学会大会
• [Presentation] Molecular taxonomy and parasitic specialization in Pyriculariaceae. (2017)
  • Author(s): Chuma, I., T. Aoki, Y. Tosa
  • Organizer: 2017 Taiwan-Japan Mycological Research Conference.
  • Int'l Joint Research / Invited
• [Presentation] Cloning of an avirulence gene of an Eleusine isolate of Pyricularia oryzae against common wheat (2016)
  • Author(s): Asuke, S., N.J. Magculia, I. Chuma, Y. Tosa
  • Organizer: The 7th International Rice Blast Conference
  • Place of Presentation: Alabang, Philippines
  • Year and Date: 2016-10-09
  • Int'l Joint Research
• [Presentation] TALENにより異なるゲノム領域に導入されたイネいもち病菌非病原力（AVR）遺伝子の変異頻度比較解析 (2016)
  • Author(s): 平岡大輝・荒添貴之・土佐幸雄・中馬いづみ
  • Organizer: 平成28年度植物感染生理談話会
  • Place of Presentation: シーパル須磨（兵庫県・神戸市）
  • Year and Date: 2016-08-11
• [Presentation] Identification of gene pairs involved in the incompatibility between Eleusine isolates of Pyricularia oryzae and common wheat (2016)
  • Author(s): Asuke, S., I. Chuma, Y. Tosa
  • Organizer: IS-MPMI XVII Congress
  • Place of Presentation: Portland, Oregon, U.S.A.
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Remarks] 帯広畜産大学教員紹介
  • URL: https://www.obihiro.ac.jp/faculty-r/izumi-chuma