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Comparison of mutation frequency of the avirulence gene AVR-Pia introduced to different genomic region of Pyricularia oryzae.

Research Project

Project/Area Number 16K07615
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine (2018)
Kobe University (2016-2017)

Principal Investigator

Chuma Izumi  帯広畜産大学, 畜産学部, 准教授 (90628926)

Research Collaborator Arazoe Takayuki  東京理科大学
Sone Teruo  北海道大学
Le Dinh Don  ノンラム大学
Nguyen Thi Thin Nga  農業遺伝学研究所
Cumagun Christian  フィリピン大学, ロスバニョス校
Sreewongchai Tanee  カセサート大学
Spring Otmar  ホーヘンハイム大学
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsイネ科植物いもち病 / いもち病菌 / 非病原力遺伝子 / 病原性変異機構 / イネ科植物いもち病菌 / イネいもち病菌 / AVR-Pizt / 病原性変異 / 転移因子 / Pyricularia oryzae
Outline of Final Research Achievements

We compared frequency of mutation of the avirurence gene AVR-Pia which was introduced to repeat rich (RR) and repeat poor (RP) regions of rice blast fungus, Pyricularia oryzae. Resistant rice cultivar Aichi-asahi (Pia) were inoculated with transformants ta#126 which has AVR-Pia in RR region and zt#1 which has AVR-Pia in RP region. When compared mutants isolated from susceptible lesions of inoculated rice leaves, mutation frequency of ta#126 was higher than that of zt#1. Mutation mechanism of all mutants were deletion of introduced AVR-Pia. The deleted region were surrounded by transposable elements and its size depends on the distance between transposable elements.

Academic Significance and Societal Importance of the Research Achievements

世界のイネの最重要病原体のひとつであるイネいもち病菌は、イネ品種に対する病原性変異が多様であり、この変異メカニズムの解明と変異リスクの予測は、抵抗性育種への応用にも繋がる。いもち病菌が宿主に対する病原性の決定を担っているのが非病原力遺伝子であるが、これらの遺伝子の重要な変異機構としてゲノムからの欠失が挙げられる。本研究結果は、非病原力遺伝子が欠失するか否かを決定するのが周辺に存在する転移因子であり、転移因子頻度の高いゲノム領域に存在する非病原力遺伝子は欠失するリスクの高い状態にあることを示唆するものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (23 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (6 results) Journal Article (10 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] University of Phillopines Los Bagnios(フィリピン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Nong Lam University/Agricultural Genetics Institute/Plant Protection Research Institute(ベトナム)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Kasetsart University(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Philippines Los Bagnios/IRRI(フィリピン)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Nong Lam University/Agricultural Genetics Institute/Plant Protecthion Research Institute(ベトナム)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Kasetsart University(Thailand)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Blast fungal genomes show frequent chromosomal changes, gene gains and losses, and effector gene turnover.2019

    • Author(s)
      Gomez Luciano LB, Jason Tsai I, Chuma I, Tosa Y, Chen YH, Li JY, Li MY, Jade Lu MY, Nakayashiki H, Li WH.
    • Journal Title

      Mol. Biol, Evol.

      Volume: 36 Issue: 6 Pages: 1148-1161

    • DOI

      10.1093/molbev/msz045

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rmg8 and Rmg7, wheat genes for resistance to the wheat blastfungus, recognize the same avirulence gene AVR-Rmg82018

    • Author(s)
      Anh, V. Y. Inoue, S. Asuke, T.T.P. Vy, N.T. Anh, S. Wang, I. Chuma, Y. Tosa
    • Journal Title

      Mol. Plant Pthology

      Volume: 19 Issue: 5 Pages: 1252-1256

    • DOI

      10.1111/mpp.12609

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A New Resistance Gene in Combination with Rmg8 Confers Strong Resistance Against Triticum Isolates of Pyricularia oryzae in a Common Wheat Landrace2018

    • Author(s)
      Wang Shizhen、Asuke Soichiro、Vy Trinh Thi Phuong、Inoue Yoshihiro、Chuma Izumi、Win Joe、Kato Kenji、Tosa Yukio
    • Journal Title

      Phytopathology

      Volume: 108 Issue: 11 Pages: 1299-1306

    • DOI

      10.1094/phyto-12-17-0400-r

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A new resistance gene in combination with Rmg8 confers strong resistance against Triticum isolates of Pyricularia oryzae in a common wheat landrace.2018

    • Author(s)
      Wang, S., S. Asuke, T.T.P. Vy, Y. Inoue, I. Chuma, J. Win, K. Kato, Y. Tosa
    • Journal Title

      Phytopathology

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] First report of Fusarium solani species complex as a causal agent of Erythrina variegata decline and death after gall formation by Quadrastichus erythrinae on Okinawa Island, Japan.2017

    • Author(s)
      Kuroda, K., I. Chuma*, T. Kihara, T. Murakami, K. Takashina, D. Hiraoka, N. Kameyama.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 83 Pages: 344-357

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolution of the wheat blast fungus through functional losses in a host specificity determinant.2017

    • Author(s)
      Inoue, Y., T.T.P. Vy, K. Yoshida, H. Asano, C. Mitsuoka, S Asuke, V.L. Anh, C.J. Cumagun, I. Chuma, R. Terauchi, K. Kato, T. Mitchell, B. Valent, M. Farman, Y. Tosa
    • Journal Title

      Science

      Volume: 357 Pages: 80-83

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Host specialization of the blast fungus Magnaporthe oryzae is associated with dynamic gain and loss of genes linked to transposable elements.2016

    • Author(s)
      Yoshida K, Saunders DGO, Mitsuoka C, Natsume S, Kosugi S, Saitoh H, Inoue Y, Chuma I, Tosa Y, Cano LM, Kamoun S, Terauchi, R
    • Journal Title

      BMC Genomics

      Volume: 17 Pages: 370-370

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Generic names in Magnaporthales.2016

    • Author(s)
      Zhang, N., J. Luo, A.Y. Rossman, T. Aoki, I. Chuma, P.W. Crous, R. Dean, R.P. de Vries, N. Donofrio, K.D. Hyde, M.-H. Lebrun, N.J. Talbot, D. Tharreau, Y. Tosa, B. Valent, Z. Wang, and J.-R. Xu.
    • Journal Title

      IMA Fungus

      Volume: 7 Pages: 155-159

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Genetic analysis of the resistance of barley to cryptic species of Pyricularia.2016

    • Author(s)
      Tagle, A.G., I. Chuma, H. Hisano, K. Sato, and Y. Tosa
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Pages: 302-306

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Anthracnose of black locust caused by Colletotrichum nymphaeae (Passerini) Aa.2016

    • Author(s)
      Yamagishi, N., T. Sato, I. Chuma, Y. Ishiyama, and Y. Tosa
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Pages: 174-176

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] イネいもち病菌非病原力遺伝子のrepeat-poor領域における欠失2018

    • Author(s)
      平岡大輝,荒添貴之,曾根輝雄,土佐幸雄,中馬いづみ
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fusarium solani種複合体に属する菌のデイゴへの病原性と沖縄県内の地理的分布2018

    • Author(s)
      高階空也,中馬いづみ,亀山統一,黒田慶子
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular taxonomy and parasitic specialization in Pyriculariaceae.2017

    • Author(s)
      Chuma, I., T. Aoki, Y. Tosa
    • Organizer
      2017 Taiwan-Japan Mycological Research Conference.
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Cloning of an avirulence gene of an Eleusine isolate of Pyricularia oryzae against common wheat2016

    • Author(s)
      Asuke, S., N.J. Magculia, I. Chuma, Y. Tosa
    • Organizer
      The 7th International Rice Blast Conference
    • Place of Presentation
      Alabang, Philippines
    • Year and Date
      2016-10-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] TALENにより異なるゲノム領域に導入されたイネいもち病菌非病原力(AVR)遺伝子の変異頻度比較解析2016

    • Author(s)
      平岡大輝・荒添貴之・土佐幸雄・中馬いづみ
    • Organizer
      平成28年度植物感染生理談話会
    • Place of Presentation
      シーパル須磨(兵庫県・神戸市)
    • Year and Date
      2016-08-11
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification of gene pairs involved in the incompatibility between Eleusine isolates of Pyricularia oryzae and common wheat2016

    • Author(s)
      Asuke, S., I. Chuma, Y. Tosa
    • Organizer
      IS-MPMI XVII Congress
    • Place of Presentation
      Portland, Oregon, U.S.A.
    • Year and Date
      2016-07-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 帯広畜産大学教員紹介

    • URL

      https://www.obihiro.ac.jp/faculty-r/izumi-chuma

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-02-16  

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