| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
A naturally occurring cucumber mutant, A192-18 shows a highly resistant against some potyviruses. The novel recessive resistance is determined by the mutation of a gene encoding a vacuolar protein sorting factor (Vps). The virus accumulation was drastically decreased in the susceptible cucumber leaves by coexpression of mutated Vps isolated from A192-18. Furthermore, A192-18 plants permitted the virus infection by coexpression of Vps isolated from the susuceptible cucumber. A single-nucleotide change in two nucleotide mutations of the Vps gene from A192-18 conferred resistance to the viruses, and caused a cancellation of interaction with viral proteins.
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