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A new technology for creating plant virus resistance by protein-protein interaction of RNA replicase and genome editing of host factor gene

Research Project

Project/Area Number 16K07623
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionMeiji University

Principal Investigator

Kuwata Shigeru  明治大学, 農学部, 専任教授 (20328967)

Co-Investigator(Kenkyū-buntansha) 大里 修一  明治大学, 農学部, 専任准教授 (30533228)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsタンパク質-タンパク質間相互作用 / 植物ウイルス / ジャガイモYウイルス / キュウリモザイクウイルス / ゲノム編集 / 植物ウイルス抵抗性 / アルファルフアモザイクウイルス / RNA編集 / RNA複製酵素 / RNA切断酵素 / ウイルスRNA複製酵素タンパク質 / 植物病理学 / 植物ウイルス学 / タンパク質相互作用
Outline of Final Research Achievements

To develop a new technology for creating plant virus resistance, we searched for peptides that bind to catalytic domains of RNA replicase and created plants that have deletions of a host factor for viral multiplication. We isolated 16 different peptides that bind to methyltransferase, helicase and polymerase domains of cucumber mosaic virus RNA replicase. In addition, genome editing was performed to create tobacco plants with a functional destruction of the tobacco eIF4E1 gene that is required for potato Y virus (PVY) multiplication. The tobacco plants showed strong resistance to PVY, demonstrating that conferring resistance to the virus can be achieved by destroying the host factor gene required by the virus.

Academic Significance and Societal Importance of the Research Achievements

植物ウイルスの被害を防止するために、ウイルス遺伝子配列を用いた遺伝子組換え作物が作られている。しかし、ウイルス遺伝子で形質転換した作物は新規のウイルスを生み出しかねないとの懸念などから、実用化されている例は少ない。そこで、ウイルス複製を阻害するペプチド農薬の探索やウイルス複製に必要な植物の宿主因子をゲノム編集によって欠損させた植物の作製を行い、植物ウイルスの被害を軽減化する新技術を開発した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (36 results)

All 2019 2018 2017 2016

All Journal Article (14 results) (of which Peer Reviewed: 10 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (22 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Inhibition of Fusarium trichothecene biosynthesis by yeast extract components extractable with ethyl acetate2019

    • Author(s)
      Tanaka Yuya、Nakajima Yuichi、Maeda Kazuyuki、Matsuyama Momoko、Kanamaru Kyoko、Kobayashi Tetsuo、Ohsato Shuichi、Kimura Makoto
    • Journal Title

      International Journal of Food Microbiology

      Volume: 289 Pages: 24-29

    • DOI

      10.1016/j.ijfoodmicro.2018.08.026

    • NAID

      120006844194

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Germination and appressorium formation of Pyricularia oryzae Cavara can be inhibited by reduced concentration of Blasin?Flowable with carbon dioxide microbubbles2018

    • Author(s)
      Masahiko Tamaki、Fumiyuki Kobayashi、Keisuke Suehiro、Shuichi Ohsato、Michio Sato
    • Journal Title

      Journal of Integrative Agriculture

      Volume: 17 Issue: 9 Pages: 2024-2030

    • DOI

      10.1016/s2095-3119(18)61962-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum2018

    • Author(s)
      Maeda Kazuyuki、Ichikawa Hinayo、Nakajima Yuichi、Motoyama Takayuki、Ohsato Shuichi、Kanamaru Kyoko、Kobayashi Tetsuo、Nishiuchi Takumi、Osada Hiroyuki、Kimura Makoto
    • Journal Title

      ACS Chemical Biology

      Volume: 13 Issue: 5 Pages: 1260-1269

    • DOI

      10.1021/acschembio.8b00044

    • NAID

      120006523680

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis2018

    • Author(s)
      Shiobara Takuya、Nakajima Yuichi、Maeda Kazuyuki、Akasaka Manami、Kitou Yoshiyuki、Kanamaru Kyoko、Ohsato Shuichi、Kobayashi Tetsuo、Nishiuchi Takumi、Kimura Makoto
    • Journal Title

      Antonie van Leeuwenhoek

      Volume: 112 Issue: 3 Pages: 471-478

    • DOI

      10.1007/s10482-018-1172-z

    • NAID

      120006844195

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on the mechanisms of pathogenic changes of plant viruses2018

    • Author(s)
      Kuwata Shigeru
    • Journal Title

      Journal of General Plant Pathology

      Volume: 84 Issue: 6 Pages: 437-440

    • DOI

      10.1007/s10327-018-0812-5

    • NAID

      130007472319

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Studies on the mechanisms of pathogenic changes of plant viruses2018

    • Author(s)
      KUWATA S.
    • Journal Title

      Japanese Journal of Phytopathology

      Volume: 84 Issue: 3 Pages: 139-142

    • DOI

      10.3186/jjphytopath.84.139

    • NAID

      130007472319

    • ISSN
      0031-9473, 1882-0484
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Development of a method to produce chromosome lacking lines (CLLs) in Nicotiana tabacum L. “Red Russian.2017

    • Author(s)
      1.Liu H. Ito Y., Muraida N., Hayakawa Y., Itoyama K., Ohsato S, Marubashi W.
    • Journal Title

      Journal of Plant Sciences

      Volume: 8 Issue: 12 Pages: 2923-2943

    • DOI

      10.4236/ajps.2017.812198

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased metabolite production by deletion of an HDA1-type histone deacetylase in the phytopathogenic fungi, Magnaporthe oryzae (Pyricularia oryzae) and Fusarium asiaticum.2017

    • Author(s)
      2.Maeda K., Izawa M., Nakajima Y., Jin Q., Hirose T., Nakamura T., Koshino H., Kanamaru K., Ohsato S., Kamakura T., Kobayashi T., Yoshida M., Kimura M.
    • Journal Title

      Lett. Appl. Microbiol.

      Volume: 65 Issue: 5 Pages: 446-452

    • DOI

      10.1111/lam.12797

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a trichothecene production inhibitor by chemical array and library screening using trichodiene synthase as a target protein.2017

    • Author(s)
      3.Maeda K., Nakajima Y., Motoyama T., Kondoh Y., Kawamura T., Kanamaru K., Ohsato S., Nishiuchi T., Yoshida M., Osada H., Kobayashi T., Kimura M.
    • Journal Title

      Pestic. Biochem. Physiol

      Volume: 138 Pages: 1-7

    • DOI

      10.1016/j.pestbp.2017.03.006

    • NAID

      120006523682

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant.2017

    • Author(s)
      Osamu Mizutani, Takayuki Arazoe, Kenji Toshida, Risa Hayashi, Shuichi Ohsato, Tetsushi Sakuma, Takashi Yamamoto, Shigeru Kuwata, Osamu Yamada
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 123 Pages: 287-293

    • NAID

      40021152739

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular genetic characterization of Fusarium graminearum genes identified as encoding a precocene II-binding protein.2017

    • Author(s)
      Kazuyuki Maeda, Shuichi Ohsato
    • Journal Title

      JSM Mycotoxins

      Volume: 67 Pages: 1-3

    • NAID

      130005404237

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Plant viral translation strategies and disease resistance conferred by recessive host genes2016

    • Author(s)
      Shigeru Kuwata
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Issue: 6 Pages: 318-322

    • DOI

      10.1007/s10327-016-0684-5

    • Related Report
      2016 Research-status Report
    • Acknowledgement Compliant
  • [Journal Article] 植物ウイルスのタンパク質翻訳戦略と抵抗性について2016

    • Author(s)
      桑田 茂
    • Journal Title

      日本植物病理学会報

      Volume: 82 Pages: 149-152

    • NAID

      130007331088

    • Related Report
      2016 Research-status Report
  • [Journal Article] Novel avidin-like proteins with low isoelectric points from shiitake mushroom (Lentinula edodes).2016

    • Author(s)
      Yoshimitsu Takakura, Kozue Sofuku, Masako Tsunashima, Shigeru Kuwata
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 121 Pages: 420-423

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 組換えベントグラス病原性フザリウム病菌の蛍光観察と拮抗性種子随伴菌の分離2019

    • Author(s)
      前田一行,中山翔太,鄒馨逸,中嶋佑一,木村真,大里修一
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of expression pattern of trichothecene deacetyl esterase, Tri1042019

    • Author(s)
      前田一行,富永直樹,兵藤壮一郎,杉浦涼介,服部明日希,松山桃子,大里修一,鎌倉高志,木村真
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ベントグラスから分離された芝草病原性フザリウム病菌の感染生理および生態に関する研究2018

    • Author(s)
      前田一行,山下将,中嶋佑一,木村真,大里修一
    • Organizer
      2018年度日本芝草学会春期大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 培養時に多様なアセチル化型トリコテセンを生産するNBRC 9955株の遺伝的背景の解析2018

    • Author(s)
      前田一行,田中佑弥,中嶋佑一,新海航輝,松井宏介,足立健太郎,安藤直子,木村真,大里修一
    • Organizer
      日本マイコトキシン学会第81回学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネいもち病菌のRNAサイレンシング関連遺伝子のMoCV1-A感染への影響2018

    • Author(s)
      高野翔,木口歌菜,高崎恵利花,有江力,森山裕充,大里修一,小松健
    • Organizer
      第18回 糸状菌分子生物コンファレンス
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネいもち病菌の相同組換え関連遺伝子破壊株におけるDNAリセクション反応検出系の構築に向けて2018

    • Author(s)
      木口歌菜,田中寿樹,荒添貴之,佐久間哲史,山本卓,桑田茂,大里修一
    • Organizer
      平成30年度日本植物病理学会九州部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 二分子蛍光補完法を利用したイネいもち病菌における細胞膜透過ペプチド評価法の構築2018

    • Author(s)
      太田光祐,富田健一, 前田一行, 桑田茂, 大里修一
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物ウイルスの病原性変異機構に関する研究2018

    • Author(s)
      桑田 茂
    • Organizer
      平成30年度日本植物病理学会大会 学会賞受賞講演 神戸国際会議場
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Functional analysis of RecQ helicase MUSN in Pyricularia oryzae,2017

    • Author(s)
      1.Kana Kiguchi, Toshiki Tanaka, Takayuki Arazoe, Tetsushi Sakuma, Takashi Yamamoto, Sigeru Kuwata, Shuichi Ohsato,
    • Organizer
      Asian Conference on Plant Pathology, Korea, International Convention Center, Jeju (ICC Jeju)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] CRISPR/Cas9 system for genome editing in filamentous fungi2017

    • Author(s)
      Takayuki Arazoe, Tohru Yamato, Ai Handa, Kennosuke Miyoshi, Shuichi Ohsato and Shigeru Kuwata,
    • Organizer
      , 4th Japan-Korea Joint Symposium on Plant Pathology & KSPP Annual Meeting International Convention Center Jeju, Korea,
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] CRISPR/Cas9システムを利用した相同組換えによる多重遺伝子同時改変の試み2017

    • Author(s)
      飯田藍、大和澄、荒添貴之、大里修一、桑田茂
    • Organizer
      平成29年度日本植物病理学会大会 岩手県民情報交流センター、盛岡市
    • Related Report
      2017 Research-status Report
  • [Presentation] A型トリコテセン類を生産するNBRC 9955株の再同定と培養特性の解析2017

    • Author(s)
      2.前田一行、田中佑弥、中嶋佑一、新海航輝、松井宏介、足立健太郎、安藤直子、木村真、大里修一
    • Organizer
      日本マイコトキシン学会第80回学術講演会、パルテノン多摩・小ホール
    • Related Report
      2017 Research-status Report
  • [Presentation] イネいもち病菌におけるDna2遺伝子の破壊と性状解析2017

    • Author(s)
      3.國吉真史、田中寿樹、木口歌菜、高崎恵利花、荒添貴之、佐久間哲史、山本卓、桑田茂、大里修一
    • Organizer
      日本植物病理学会関東部会、横浜国立大学 教育文化ホール
    • Related Report
      2017 Research-status Report
  • [Presentation] 膜透過性ペプチドを用いたイネいもち病菌へのEGFP送達と経路2017

    • Author(s)
      4.冨田健一、太田光祐、桑田茂、大里修一
    • Organizer
      日本植物病理学会関東部会、横浜国立大学 教育文化ホール
    • Related Report
      2017 Research-status Report
  • [Presentation] ゲノム改変技術によるサトウキビの育種について2017

    • Author(s)
      5.西橋潤、近藤聡、大音徳、太田邦史、桑田茂、大里修一
    • Organizer
      日本植物病理学会九州部会、沖縄県立博物館・美術館
    • Related Report
      2017 Research-status Report
  • [Presentation] 新規ゲノム改変育種技術を用いたイネ耐病性系統取得の試み2017

    • Author(s)
      6.酒井皓大、三宅駿史、栗原美樹、近藤聡、村本伸彦、光川典宏、大音徳、太田邦史、桑田茂、大里修一
    • Organizer
      日本植物病理学会九州部会、沖縄県立博物館・美術館
    • Related Report
      2017 Research-status Report
  • [Presentation] イネいもち病菌における菌糸融合検出系の構築に向けて2017

    • Author(s)
      7.松尾涼平、前田一行、加納寛也、桑田茂、大里修一
    • Organizer
      日本植物病理学会九州部会、沖縄県立博物館・美術館
    • Related Report
      2017 Research-status Report
  • [Presentation] イネいもち病菌RecQ helicase MUSNのDNA修復機構への関与2017

    • Author(s)
      8.木口歌菜、田中寿樹、國吉真史、荒添貴之、佐久間哲史、山本卓、桑田茂、大里修一
    • Organizer
      第17回 糸状菌分子生物コンファレンス2017
    • Related Report
      2017 Research-status Report
  • [Presentation] イネいもち病菌における菌糸融合検出系の検討と関連遺伝子の解析2017

    • Author(s)
      9.松尾涼平、前田一行、桑田茂、大里修一
    • Organizer
      第17回 糸状菌分子生物コンファレンス2017、佐賀市立東与賀文化ホール
    • Related Report
      2017 Research-status Report
  • [Presentation] イネいもち病菌におけるRecQ helicase MUSNのDNA損傷応答への関与2016

    • Author(s)
      田中寿樹,國吉真史,高崎恵利花,小川哲央,桑田茂,大里修一
    • Organizer
      平成28年度日本植物病理学会九州部会
    • Place of Presentation
      佐賀市:グランデはがくれ
    • Year and Date
      2016-11-09
    • Related Report
      2016 Research-status Report
  • [Presentation] イネいもち病菌の相同組換え修復に関与するSRS2複合体の相互作用領域2016

    • Author(s)
      富田成美,小川哲央,荒添貴之,桑田茂,草野好司,大里修一
    • Organizer
      平成28年度日本植物病理学会関東部会
    • Place of Presentation
      横浜市:横浜国立大学
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 膜透過性ペプチドPenetratinとイネいもち病菌へのタンパク質の直接導入法2016

    • Author(s)
      太田光祐,冨田健一,桑田茂,大里修一
    • Organizer
      平成28年度日本植物病理学会関東部会
    • Place of Presentation
      横浜市:横浜国立大学
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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