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Molecular ecological study on gene duplication and insecticide resistance in diamondback moth

Research Project

Project/Area Number 16K07624
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionUtsunomiya University

Principal Investigator

Sonoda Shoji  宇都宮大学, 農学部, 教授 (00325127)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords殺虫剤抵抗性 / ジアミド剤 / コナガ / フルベンジアミド / クロラントラニリプロール / アミノ酸変異 / リアノジン受容体 / 量的シーケンシング / 遺伝子重複 / 変異 / 昆虫
Outline of Final Research Achievements

A method to estimate proportions of the G4946E mutation in the ryanodine receptor gene, a major mechanism of diamide insecticide resistance, was developed for diamondback moth populations using quantitative sequencing (QS). Using the QS-based method, the relationship between proportions of the G4946E mutation and mortalities for diamide was examined using field-collected populations. Results showed that the populations might be classifiable according to the proportions. The seasonal proportion change of the G4946E mutation for diamondback moth populations was examined using the QS-based method. Results showed that the proportions of the G4946E mutation generally increased from spring through summer, but then decreased in autumn. After termination of diamide use, noticeable decrease of the proportions of the G4946E mutation was observed, suggesting fitness cost of the mutation.

Academic Significance and Societal Importance of the Research Achievements

コナガのジアミド剤抵抗性に関わるG4946Eの頻度を個体群レベルで解析するための手法を初めて開発した。この手法を用いて抵抗性レベルの異なる野外個体群のG4946E頻度を調べ、ジアミド剤はG4946E頻度が40%未満の個体群に対して防除効果が期待できることを明らかにした。また、野外個体群のG4946E頻度の季節的な変化を調べ、晩秋のG4949E頻度と翌春のG4946E頻度はほぼ同じであることから、当該年のジアミド剤の効果は前年のG4946E頻度に基づいてあらかじめ予測できることを明らかにした。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Invited: 1 results) Funded Workshop (1 results)

  • [Journal Article] Molecular evaluation of diamide resistance in diamondback moth (Lepidoptera: Yponomeutidae) populations using quantitative sequencing2017

    • Author(s)
      Shoji Sonoda, Kayo Inukai, Satoshi Kitabayashi, Seigo Kuwazaki, Akiya Jouraku
    • Journal Title

      Applied Entomology and Zoology

      Volume: 52 Issue: 2 Pages: 353-357

    • DOI

      10.1007/s13355-017-0482-3

    • Related Report
      2017 Research-status Report
  • [Journal Article] Seasonal proportion change of ryanodine receptor mutation (G4946E) in diamondback moth populations2017

    • Author(s)
      Yuki Itagaki, Shoji Sonoda
    • Journal Title

      Journal of Pesticide Science

      Volume: 42 Issue: 3 Pages: 116-118

    • DOI

      10.1584/jpestics.D17-033

    • NAID

      130005988564

    • ISSN
      1348-589X, 1349-0923
    • Related Report
      2017 Research-status Report
  • [Journal Article] Genotyping for the G4946E site of ryanodine receptor gene in Plutella xylostella (Lepidoptera: Yponomeutidae) cnsidering gene duplication2016

    • Author(s)
      Sonoda, S, Kataoka, Y
    • Journal Title

      Applied Entomology and Zoology

      Volume: 51 Issue: 2 Pages: 195-204

    • DOI

      10.1007/s13355-015-0385-0

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] コナガ個体群におけるジアミド剤抵抗性の主要因であるリアノジン受容体遺伝子変異頻度の季節的な変化2018

    • Author(s)
      園田昌司・板垣有紀
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] コナガ個体群におけるジアミド剤抵抗性の主要因であるリアノジン受容体遺伝子頻度の季節的変化2018

    • Author(s)
      園田昌司, 板垣有紀
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 量的シーケンシングを用いたコナガ個体群のジアミド剤抵抗性に関する分子診断2017

    • Author(s)
      園田昌司, 犬飼佳代, 北林聡, 桑崎誠剛, 上樂明也
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 薬剤抵抗性のメカニズムと管理に向けた試み2017

    • Author(s)
      園田昌司
    • Organizer
      四国植物防疫研究協議会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] コナガのジアミド剤抵抗性管理2016

    • Author(s)
      園田昌司
    • Organizer
      第39回C-Bioセミナー
    • Place of Presentation
      宇都宮大学バイオサイエンス教育研究センター
    • Year and Date
      2016-05-20
    • Related Report
      2016 Research-status Report
  • [Funded Workshop] XXV International Congress of Entomology2016

    • Place of Presentation
      Orland, FL, USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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