| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The discrimination method of IYSV genotypes using the single nucleotide polymorphism (SNP) was developed for the study on the possibility emerging reassortants in plants and thrips The local lesions were developed on total eight NL x BR combination. The genotyping of these local lesion indicated that all RNA segment can coexist in a local lesion, although the isolates consisted from either genotype were predominated. As second trial, viruliferous adult thrips were tested. As result, genotype in a half of thrips (5 sample) was classified to NL type and either reassortant or ‘mosaic’ ones was detected from the other half. Typical IYSV symptom was occurred on nine impatiens leaf samples inoculated using viral acquired thrips. The genotype of these all leaf sample was classified to BR type and no detected reassortant and ‘mosaic’ ones. These results suggest that the recombinant can emerge in infected plants and viruliferous thrips but is unstable and get rid of the nature.
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