| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
Many specific-MOA-fungicides have been developed and contributed to crop and vegetable production though disease control, however, the problem of fungicide resistance becomes serious and more complicated in many fungal plant pathogens particularly of cucumber and tomato. In this study, we constructed the pyrosequencing assays and the suspension-array assays for the fungicide-resistance mutations in various plant pathogen such as Botrytis cinerea and Corynespora cassiicola. Pyrosequencing assays were useful not only to detect the fungicide resistance mutations in each isolate of plant pathogen, but also to estimate the frequency of fungicide resistance alleles in pooled DNA. In contrast, the suspension-array assays could be used to determine the fungicide resistance mutations in several fungicide-target genes in a single reaction. The combination of these two tools contributes to monitoring and management of the fungicide-resistance pathogens in each field.
|
