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Roles of glutathione transport on sulfur and heavy metal movements

Research Project

Project/Area Number 16K07639
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Ohkama-Ohtsu Naoko  東京農工大学, グローバルイノベーション研究院, 教授 (40513437)

Research Collaborator Wongkaewa Arunee  
Nakamura Shin-ichi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsグルタチオン輸送体 / 植物 / カドミウム / 亜鉛 / 篩管輸送 / 重金属 / ファイトケラチン / グルタチオン / トランスポーター / シロイヌナズナ
Outline of Final Research Achievements

Organic sulfur in plants is mainly transported in the form of Glutathione, a tripeptide of gamma;-Glu-Cys-Gy. In this study we demonstrated that AtOPT6 is the glutathione transporter involved in transport of glutathione via pholoem, transport of cadmium, germination and development of reproductive organs. Furthermore we showed that Zn transport into shoots are increased by overexpressing AtOPT6 in phloem. Our study shows that regulation of AtOPT6 will lead to change movement of toxic metals and important mineral metals for human.

Academic Significance and Societal Importance of the Research Achievements

グルタチオンの篩管輸送は硫黄、窒素、重金属輸送に関与することが示唆されていたが、本研究はそれを担うグルタチオン輸送体を同定し、それが植物の生育や重金属動態に関与することを示した初めての例である。本研究の成果は、人間の健康に有用なミネラル金属成分を作物可食部で増加させるbio-fortificationや、有害重金属を減少させる方法につながる可能性がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 2 results) Book (2 results) Remarks (4 results)

  • [Int'l Joint Research] ミズーリ大学(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ミズーリ大学(米国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] University of Missouri(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] AtOPT6 Protein Functions in Long Distance Transport of Glutathione in Arabidopsis thaliana2018

    • Author(s)
      Arunee Wongkaew, Koki Asayama, Taisuke Kitaiwa, Shin-ichi Nakamura, Katsuhiro Kojima, Gary Stacey, Hitoshi Sekimoto, Tadashi Yokoyama and Naoko Ohkama-Ohtsu
    • Journal Title

      Plant and cell physiology

      Volume: in press Pages: 1443-1451

    • DOI

      10.1093/pcp/pcy074

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Elevated glutathione synthesis in leaves contributes to zinc transport from roots to shoots in Arabidopsis2018

    • Author(s)
      Wongkaew Arunee、Nakamura Shin-ichi、Suzui Nobuo、Yin Yong-Gen、Ishii Satomi、Kawachi Naoki、Kojima Katsuhiro、Sekimoto Hitoshi、Yokoyama Tadashi、Ohkama-Ohtsu Naoko
    • Journal Title

      Plant Science

      Volume: - Pages: 416-423

    • DOI

      10.1016/j.plantsci.2018.11.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Foliar-applied glutathione activates zinc transport from roots to shoots in oilseed rape2018

    • Author(s)
      Nakamura Shin-ichi、Wongkaew Arunee、Nakai Yuji、Rai Hiroki、Ohkama-Ohtsu Naoko
    • Journal Title

      Plant Science

      Volume: - Pages: 424-434

    • DOI

      10.1016/j.plantsci.2018.10.018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of C-terminal Regions in Arabidopsis thaliana Phytochelatin Synthase 1 Specifically Involved in Activation by Arsenite2017

    • Author(s)
      Uraguchi Shimpei、Sone Yuka、Ohta Yumika、Ohkama-Ohtsu Naoko、Hofmann Christian、Hess Natalia、Nakamura Ryosuke、Takanezawa Yasukazu、Clemens Stephan、Kiyono Masako
    • Journal Title

      Plant and cell physiology

      Volume: 59 Issue: 3 Pages: 500-509

    • DOI

      10.1093/pcp/pcx204

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Phytochelatin Synthase has Contrasting Effects on Cadmium and Arsenic Accumulation in Rice Grains2017

    • Author(s)
      Uraguchi Shimpei、Tanaka Nobuhiro、Hofmann Christian、Abiko Kaho、Ohkama-Ohtsu Naoko、Weber Michael、Kamiya Takehiro、Sone Yuka、Nakamura Ryosuke、Takanezawa Yasukazu、Kiyono Masako、Fujiwara Toru、Clemens Stephan
    • Journal Title

      Plant and cell physiology

      Volume: 58 Issue: 10 Pages: 1730-1742

    • DOI

      10.1093/pcp/pcx114

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plants prioritize phytochelatin synthesis during cadmium exposure even under reduced sulfate uptake caused by the disruption of SULTR1;22017

    • Author(s)
      Yamaguchi Chisato、Ohkama-Ohtsu Naoko、Shinano Takuro、Maruyama-Nakashita Akiko
    • Journal Title

      Plant Signaling and Behavior

      Volume: 12 Issue: 5 Pages: 1325053-1325053

    • DOI

      10.1080/15592324.2017.1325053

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Assessment of glutathione transporter AtOP6 functions on sink organs in Arabidopsis2018

    • Author(s)
      Arunee Wongkaew・Shin-ichi Nakamura・Tadashi Yokoyama・Naoko Ohkama-Ohtsu
    • Organizer
      日本土壌肥料学会神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] グルタチオン分解経路の機能分化と生理的役割の解析2017

    • Author(s)
      西薗亘祐・北岩泰祐・横山正・大津直子
    • Organizer
      日本土壌肥料学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 亜ヒ酸応答におけるAtPCS1のC末端領域の機能解析2017

    • Author(s)
      浦口晋平,曽根有香,太田有美佳,大津(大鎌)直子,中村亮介,高根沢康一,Stephan Clemens, 清野正子
    • Organizer
      日本土壌肥料学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] シロイヌナズナのグルタチオン輸送体がカドミウム輸送と花茎形成に果たす役割2017

    • Author(s)
      朝山紘貴・北岩泰祐・ 中村進一・横山正 ・ 大津(大鎌)直子
    • Organizer
      日本土壌肥料学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] The inner and outer enhancement of glutathione on Zn behavior in plants2017

    • Author(s)
      Arunee Wongkaewa, S. Nakamurab, N. Suzuic, Y.-G. Yinc, S Ishiic, S Fujimakic, N. Kawachic, N. Ohkama-Ohtsu
    • Organizer
      日本土壌肥料学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Mechanisms of cadmium-induced increases of sulfate uptake, translocation and thiol synthesis in Arabidopsis thaliana2017

    • Author(s)
      Chisato Yamaguchi, Yuki Takimoto, Naoko Ohkama-Ohtsu, Akiko Hokura, Takuro Shinano, Toshiki Nakamura, Akiko Suyama, Akiko Maruyama-Nakashita
    • Organizer
      TAIWAN-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞内グルタチオン分解酵素の同定と硫黄代謝における役割の解析2016

    • Author(s)
      北岩泰祐・横山 正・大津(大鎌)直子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      佐賀県佐賀市佐賀大学
    • Related Report
      2016 Research-status Report
  • [Presentation] Effects of Cadmium Treatment on the Uptake and Translocation of Sulfate in Arabidopsis thaliana.2016

    • Author(s)
      Chisato Yamaguchi, Yuki Takimoto, Naoko Ohkama-Ohtsu, Akiko Hokura, Takuro Shinano, Toshiki Nakamura, Akiko Suyama and Akiko Maruyama-Nakashita
    • Organizer
      5th Sulphyton Workshop: “Sulfur Nutrition and Metabolism in Plants"
    • Place of Presentation
      University of Taipei (台北、台湾)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Physiological roles of a rice phytochelatin synthase gene OsPCS1 in cadmium and arsenic tolerance.2016

    • Author(s)
      浦口晋平・田中伸裕・Christian Hofmann・Stephan Hoereth・Michael Weber・神谷岳洋・大津直子・安孫子果歩・曽根有香・中村亮介・高根沢康一・清野正子・Stephan Clemens・藤原 徹
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      佐賀県佐賀市佐賀大学
    • Related Report
      2016 Research-status Report
  • [Book] Chapter 18: Sulfur Assimilation and Glutathione Metabolism in Plants In "Glutathione in Plant Growth, Development, and Stress Tolerance"2017

    • Author(s)
      Akiko Maruyama-Nakashita, Naoko Ohkama-Ohtsu
    • Total Pages
      22
    • Publisher
      Springer
    • ISBN
      9783319666815
    • Related Report
      2017 Research-status Report
  • [Book] Glutathione in plant growth, development and stress tolerance2017

    • Author(s)
      Akiko Maruyama-Nakashita, Naoko Ohkama-Ohtsu
    • Publisher
      Springer Science+Business Media
    • Related Report
      2016 Research-status Report
  • [Remarks] http://web.tuat.ac.jp/~plantnut/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 研究者プロフィール

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/32/0003141/profile.html

    • Related Report
      2017 Research-status Report
  • [Remarks] 大津 直子 - 東京農工大学研究者情報検索

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/32/0003141/profile.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 東京農工大学植物栄養学研究室

    • URL

      http://web.tuat.ac.jp/~plantnut/

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2022-02-16  

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