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Evaluation of soil microbial communities and chemical properties in fruit orchards across all of Japan for the establishment of sustainable agriculture

Research Project

Project/Area Number 16K07645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKyoto Prefectural University

Principal Investigator

Cruz Andre Freire  京都府立大学, 生命環境科学研究科, 講師 (80405240)

Co-Investigator(Kenkyū-buntansha) 矢内 純太  京都府立大学, 生命環境科学研究科, 教授 (00273491)
福田 秀美  京都大学, 情報学研究科, 助教 (40726361)
中尾 淳  京都府立大学, 生命環境科学研究科, 准教授 (80624064)
Research Collaborator Yanai Junta  
Fukuda Hidemi  
Nakao Atsushi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords土壌微生物 / 多様性 / 酵素活性 / 土壌微生物バイオマス / 土壌化学 / 果樹園 / Fruit / Soil / Microbial community / Soil Fertility / Enzyme activity / Microbial biomass / Amplicon analysis / Orchard / Microbiology / Metagenome / bioinformatic / Fertility / Bacteria / Fungi / Chemistry / Agriculture / Fruits
Outline of Final Research Achievements

The soils from fruit orchards from all over japan had their microbial community (bacterial and fungal) determined by amplicon gene analysis (Illumina MiSeq 250bp). The soil microbial biomass (C, N, P and K) and activity, represented by enzymes (Phosphatase, Dehydrogenase, Arylsulphatase, b-gluosidase, protease and Urease) were also determined. Furthermore, soil chemical properties: pH, Total N, Total P, Available P, Amourphous fraction, and EC were analysed. The abundance, diversity, and composition of the microbial communities were specific according to fruit type and region (Location). The estimated microbial richness was generally higher in northern regions than in those from southern. Similar pattern was found for microbial biomass N and C, and enzyme activity. Considering the relationship between these data, the enzyme activities were positively correlated to bacterial richness, but not with fungal ones. Also, the soil microbial biomass P had correlation with amourphous fraction.

Academic Significance and Societal Importance of the Research Achievements

This research could demonstrate that the soil characteristics (microbial community, biomass, activity and chemical properties) can range according to land using in fruit orchards. Especially the high values of diversity and enzyme activity could confirm the influence of organic matter.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 Other

All Int'l Joint Research (6 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (2 results)

  • [Int'l Joint Research] University of the Phillipines Los Banos(フィリピン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Universidade de Brasilia(ブラジル)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Makerere University(ウガンダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Tadulako University(インドネシア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Makerere University(Uganda)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Agricultural Res. Co. Minas Gerais(Brazil)

    • Related Report
      2016 Research-status Report
  • [Presentation] The soil microbial status as a crucial indicator of sustainable agriculture at local and global scale2019

    • Author(s)
      Andre Freire Cruz
    • Organizer
      1st International Research Conference - Manila - The Philippines
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Survey of the bacterial community in soils of fruit orchards from all over Japan.2018

    • Author(s)
      Andre Freire Cruz, Atsushi Nakao, Junta Yanai
    • Organizer
      World Congress of Soil Science - Rio de Janeiro - Brazil
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 7th International Conference on Environmental Microbiology, Soil Microbiology & Microbial biogeochemistry”, and “2nd International Conference on Microbial Ecology & Ecosystems”, and “2nd International Conference on Ecology, Ecosystems and Conservation Biology2018

    • Author(s)
      Andre Freire Cruz, Tetsushi Tanaka, Junta Yanai, Naoaki Ono, Shigehiko Kanaya
    • Organizer
      Operational taxonomic units that distinguish soils of crops and fruits in the whole Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 日本の果樹園における土壌微生物活性の評価-卒論発表

    • Related Report
      2017 Research-status Report
  • [Remarks] メタゲノム解析による日本果樹園土壌微生物群集の評価-卒論発表

    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-07-01  

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