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Elucidation of the ecological roles of non-chitin-degrading microorganisms in chitin-added soil

Research Project

Project/Area Number 16K07648
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionShizuoka Institute of Science and Technology

Principal Investigator

Saito Akihiro  静岡理工科大学, 理工学部, 教授 (50375614)

Co-Investigator(Kenkyū-buntansha) 鮫島 玲子  静岡大学, 農学部, 准教授 (00377722)
小谷 真也  静岡大学, 農学部, 准教授 (20510621)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsキチン / アセチルグルコサミン / 土壌改良 / キチナーゼ / N,N'-ジアセチルキトビオース / プロテオバクテリア / 病害防除 / ジアセチルキトビオース / ストレプトマイセス / Streptomyces / バチルス / Baillus / Paenibacillus / 抗菌活性 / 細菌 / キチン分解 / 細菌群集構造 / バイオーム / 細菌間相互作用 / 細菌群集構造解析 / 土壌 / 多糖 / 酵素 / 抗生物質 / マイクロバイオーム
Outline of Final Research Achievements

To clarify the ecological significance of non-chitin-degrading bacteria in chitin-supplemented soil, bacteria isolated from field soil cultured for 45 days with the addition of chitin were analyzed. All the obtained bacterial strains with or without chitin degradation ability could utilize chitin degradation products, either N-acetylglucosamine, N, N '-diacetylchitobiose, or both. These non-chitin-degrading bacteria were suggested to utilize chitinolytic products obtained by enzymes secreted by chitinolytic bacteria. Non-chitin-degrading bacterial strains included those closely related to bacterial strains of the classes α- and γ-Proteobacteria, which have been reported to have beneficial effects on plants.

Academic Significance and Societal Importance of the Research Achievements

畑土壌へのキチン添加によって植物の病害が低減されることが報告されてきた。その効果は,主にキチン添加によって増加するキチン分解微生物によるものとして説明されている。本研究課題では,キチン添加土壌に存在するキチン非分解細菌に焦点を当て,研究を行った。その結果,キチン非分解細菌は,キチン分解細菌が分泌生産するキチン分解酵素によって得られるキチン分解物を資化して生きていることを想像させる結果が得られた。一方,キチン非分解細菌には,植物に対して生育促進効果をもつことが報告されているものと近縁のものが含まれていた。キチン添加による植物病害低減効果の仕組みに関する新たな側面を示唆する結果が得られた。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (23 results)

All 2020 2019 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (15 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Now and future of “smart” agriculture in Shizuoka2020

    • Author(s)
      森田 明雄, 廣野 祐平, 切岩 祥和, 齋藤 明広, 甲斐 剛, 小池 誠, 中野 敬之, 二川 雅登, 名田 和義, 平塚 伸
    • Journal Title

      Japanese Journal of Soil Science and Plant Nutrition

      Volume: 91 Issue: 2 Pages: 106-111

    • DOI

      10.20710/dojo.91.2_106

    • NAID

      130007831581

    • ISSN
      0029-0610, 2424-0583
    • Year and Date
      2020-04-05
    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Journal Article] Transition of the Bacterial Community and Culturable Chitinolytic Bacteria in Chitin-treated Upland Soil: From <i>Streptomyces</i> to Methionine-auxotrophic <i>Lysobacter</i> and Other Genera2020

    • Author(s)
      Yukari Iwasaki, Tatsuya Ichino, Akihiro Saito
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME19070

    • NAID

      130007784452

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of chitin degradation products N-acetylglucosamine and N,N'-diacetylchitobiose on chitinase activity and bacterial community structure in an incubated upland soil2020

    • Author(s)
      Yusuke Shimoi, Daichi Honma, Airi Kurematsu, Yukari Iwasaki, Yohei Kotsuchibashi, Yusuke Wakikawa, Akihiro Saito
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: - Issue: 3 Pages: 429-437

    • DOI

      10.1080/00380768.2020.1767488

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermogravimetric evaluation of chitin degradation in soil: implication for the enhancement of ammonification of native organic nitrogen by chitin addition2018

    • Author(s)
      Yuta Kumeta, Kaori Inami, Kozue Ishimaru, Yasushi Yamazaki, Reiko Sameshima-Saito, Akihiro Saito
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 64 Issue: 4 Pages: 512-519

    • DOI

      10.1080/00380768.2018.1457408

    • NAID

      210000012001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the bactericidal and fungicidal activities of poly([2-(methacryloyloxy)ethyl]trimethyl ammonium chloride)(poly(METAC))-based materials2018

    • Author(s)
      Toshiki Shiga, Hiromitsu Mori, Keiichi Uemura, Ryota Moriuchi, Hideo Dohra, Aika Yamawaki-Ogata, Yuji Narita, Akihiro Saito, Yohei Kotsuchibashi
    • Journal Title

      Polymers

      Volume: 10 Issue: 9 Pages: 947-947

    • DOI

      10.3390/polym10090947

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] NgcE<sup>Sco</sup> Acts as a Lower-Affinity Binding Protein of an ABC Transporter for the Uptake of <i>N,N′</i>-Diacetylchitobiose in <i>Streptomyces coelicolor</i> A3(2)2018

    • Author(s)
      Chiharu Iinuma, Akihiro Saito, Takayuki Ohnuma, Elodie Tenconi, Adeline Rosu, Severine Colson, Yuuki Mizutani, Feng Liu, Magdalena Swiatek-Poltynska, Gilles P. van Wezel, Sebastien Rigali, Takeshi Fujii, Kiyotaka Miyashita
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 3 Pages: 272-281

    • DOI

      10.1264/jsme2.ME17172

    • NAID

      130007491795

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The concentration of iodine in horse serum and its relationship with thyroxin concentration by geological difference2016

    • Author(s)
      Mochizuki M, Hayakawa N, Minowa F, Saito A, Ishioka K, Ueda F, Okubo K, Tazaki H.
    • Journal Title

      Environmental monitoring and assessment

      Volume: 188 Issue: 4 Pages: 226-226

    • DOI

      10.1007/s10661-016-5221-7

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] N-アセチルグルコサミンとN,N'-ジアセチルキトビオースの培養畑土壌での消長と細菌群集構造への影響2019

    • Author(s)
      下位祐輔,本間大智,齋藤明広
    • Organizer
      日本キチン・キトサン学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細菌と真菌の細胞壁に由来する土壌中のN-アセチルグルコサミンの定量の試み2019

    • Author(s)
      本間大智,下位祐輔,齋藤明広
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analysis of bacterial community structure for evaluating maturation of compost for Agaricus blazei Murrill cultivation2019

    • Author(s)
      Naoto Tada, Natsumi Sugawara, Mitsuo Kawade, Akihiro Saito, Akikazu Ando
    • Organizer
      Asian Mycological Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] キチン添加土壌で増えるキチン分解細菌群の変動と相互作用2018

    • Author(s)
      岩崎由夏里、齋藤明広
    • Organizer
      日本土壌微生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 土壌でのN-アセチルグルコサミン残基の定量方法の開発2018

    • Author(s)
      本間大智、脇川祐輔、齋藤明広
    • Organizer
      日本土壌微生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] キチン添加土壌でのLysobacter属細菌の増加への放線菌の関与2018

    • Author(s)
      岩崎由夏里、齋藤明広
    • Organizer
      日本微生物生態学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 観賞魚水槽用濾材としての木質プラスチックの利用: アンモニア/亜硝酸除去能と付着細菌群集構造2018

    • Author(s)
      組田恵里、岩崎由夏里、齋藤明広
    • Organizer
      日本微生物生態学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Induction of chitinase production and transporters for N,N'-diacetylchitobiose in Streptomyces coelicolor A3(2)2018

    • Author(s)
      Akihiro Saito
    • Organizer
      14th International Chitin and Chitosan Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 酵素とGC/MSを用いた土壌中のN-アセチルヘキソサミンの検出2018

    • Author(s)
      本間大智、齋藤明広
    • Organizer
      日本土壌肥料学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 培養畑土壌でのキチン分解物の消長と細菌文集構造の変化2018

    • Author(s)
      本間大智,下位裕輔,榑松愛理,岩崎由夏里,齋藤明広
    • Organizer
      日本土壌肥料学会中部支部
    • Related Report
      2018 Research-status Report
  • [Presentation] キチン添加畑土壌でのキチン分解菌と非分解菌の挙動と相互作用2017

    • Author(s)
      岩崎由夏里、齋藤明広
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Streptomyces coelicolor A3(2)の推定N-アセチルグルコサミダーゼの解析と土壌添加2017

    • Author(s)
      本間大智、齋藤明広
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Research-status Report
  • [Presentation] キチンと低分子量キチンの畑土壌での分解特性と微生物群集構造への影響の比較解析2016

    • Author(s)
      大塚恵巳、三澤義知、樋口昌宏、齋藤明広
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2016-09-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 畑土壌での低分子量キチンの分解特性と微生物群集構造への影響2016

    • Author(s)
      大塚恵巳、三澤義知、樋口昌宏、齋藤明広
    • Organizer
      日本キチン・キトサン学会
    • Place of Presentation
      川越市
    • Year and Date
      2016-08-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 土壌中のキチン分解酵素活性と微生物に対するN-アセチルグルコサミンとN,N'-ジアセチルキトビオースの影響2016

    • Author(s)
      榑松愛理、齋藤明広
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      岐阜大学
    • Year and Date
      2016-06-11
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] ベンゾオキサボロール基を含有する抗菌性ドレッシング材2019

    • Inventor(s)
      小土橋洋平,齋藤明広
    • Industrial Property Rights Holder
      学校法人静岡理工科大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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