| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Pseudomonas chlororaphis subsp. aurantiaca StFRB508 regulates phenazine production by AHL-mediated quorum sensing. The complete genome sequence revealed the presence of triplicate quorum-sensing gene sets, designated as phzI/R, aurI/R, and csaI/R. LC-MS/MS analysis revealed that StFRB508 produces six types of AHLs and the most important AHL is 3-OH-C6-HSL. PhzI mainly catalyzes the biosynthesis of 3-OH-C6-HSL. A mutation in phzI decreased phenazine production, but that in aurI or csaI did not affect phenazine production. Phenazine production by AHL synthase gene mutant was stimulated by exogenous AHLs and 3-OH-C6-HSL showed the highest effect on phenazine production at the lowest concentration tested. These results demonstrated that the triplicate quorum-sensing system plays an important role in phenazine production in StFRB508.
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