Coordinated regulation of the expression network of genes encoding chitin-degradation and utilization system by small RNA
Project/Area Number |
16K07659
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | Niigata University |
Principal Investigator |
|
Research Collaborator |
WATANABE takeshi
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | small RNA / 遺伝子発現制御 / キチナーゼ / 細菌 / 応用微生物学 / 遺伝子発現 / 応用微生物 |
Outline of Final Research Achievements |
ChiX is a small RNA that coordinately regulates the gene expression of chitin-degrading enzymes and the uptake system of chitin degradation products in Serratia marcescens. Although ChiX suppressed the expression of chitin-degrading enzyme genes by suppressing its translation by base pairing with chiR mRNA encoding transcriptional factor, the expression of chiP led to the derepression of chiR by ChiX. The chiP mRNA was capable of base pairing with the region extending before and after the terminator of ChiX, which suggested that ChiX switched the target from chiR mRNA to chiP mRNA.
|
Academic Significance and Societal Importance of the Research Achievements |
本研究により、細菌におけるsmall RNAが介する2遺伝子間の新たな制御機構が明らかになる。この研究成果は、近年注目されているsmall RNAの新たな機能の理解やキチン分解利用機構の解明に貢献するだけでなく、細菌の新たな生命現象の解明につながる。さらには、small RNAによる遺伝子発現制御を利用した代謝改変により、細菌による新たな物質生産方法の開発などの応用に役立つと考えられる。
|
Report
(4 results)
Research Products
(10 results)