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Elucidation of hydrogen production mechanisms from succinate oxidation in a thermophilic propionate oxidizing bacterium

Research Project

Project/Area Number 16K07666
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionYamaguchi University

Principal Investigator

Kosaka Tomoyuki  山口大学, 大学院創成科学研究科, 助教 (70500453)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords共生的プロピオン酸酸化 / メタン発酵 / コハク酸脱水素酵素 / フマル酸還元酵素 / プロピオン酸酸化細菌 / 水素生成 / 膜電位 / プロピオン酸酸化 / 大腸菌異種発現 / サブユニット構造 / コハク酸デヒドロゲナーゼ / 水素放出 / 微生物代謝 / コハク酸酸化 / 高温メタン発酵
Outline of Final Research Achievements

The revealed factors required for hydrogen production from propionate in Pelotomaculum thermopropionicum were membrane potential, ATP synthetase, and quinones. On the other hand, the two succinate dehydrogenases found in the genome of P. thermopropionicum proposed as responsible for succinate oxidation in the propionate metabolism, of which membrane binding SDH1 was proposed the major succinate dehydrogenase under a propionate oxidizing condition, whereas cytoplasmic SDH2 was predicted as a fumarate reductase under a non-propionate oxidizing condition. These results suggest that protein components localized in membrane responsible for the succinate oxidation are important for the propionate metabolism in P. thermopropionicum.

Academic Significance and Societal Importance of the Research Achievements

高温メタン発酵ではプロピオン酸などの有機酸が蓄積しやすく、その代謝に重要な因子の探索は高温メタン発酵の安定化につながることが期待される。今回の研究成果によって、中温の場合と同様に高温メタン発酵においてもプロピオン酸酸化には細胞の膜電位が重要であることが示されたことから、メタン発酵槽への微生物の膜電位に影響を及ぼす化学物質の混入やそれらを生産する微生物の増殖がメタン発酵槽の不安定化を引き起こすことが示唆された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Flagellum-mediated motility in Pelotomaculum thermopropionicum SI2019

    • Author(s)
      Kosaka Tomoyuki、Goda Mutsumi、Inoue Manami、Yakushi Toshiharu、Yamada Mamoru
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: - Issue: 7 Pages: 1-10

    • DOI

      10.1080/09168451.2019.1597618

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An aggregation-defective mutant of Methanothermobacter sp. CaT2 reveals a unique protein-dependent aggregation2019

    • Author(s)
      Kana Sumikawa, Tomoyuki Kosaka, Noriaki Mayahara, Minenosuke Matsutani, Koichi Udo and Mamoru Yamada
    • Journal Title

      Microbes and Environments

      Volume: ー

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 高温性プロピオン酸酸化細菌のプロピオン酸代謝におけるコハク酸脱水素酵素の生理的役割2018

    • Author(s)
      津島 由佳、石口 貴之、高坂 智之、山田 守
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] プロピオン酸酸化細菌のプロピオン酸酸化からの水素生成に関与する因子の探索2017

    • Author(s)
      高坂智之、津島由佳、石口貴之、山田守
    • Organizer
      日本農芸化学会2017年度(平成29年度)大会
    • Place of Presentation
      京都府京都市、京都女子大
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 高温性プロピオン酸酸化細菌のコハク酸酸化に関与する酵素の機能的特徴2017

    • Author(s)
      津島由佳、 石口貴之、高坂智之、山田守
    • Organizer
      日本農芸化学会中四国支部第47回講演会(例会)
    • Place of Presentation
      島根県松江市、島根大学
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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