| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Myxococcus xanthus increased diadenosine polyphosphate (Ap4A and Ap5A) by several to five times under various stress conditions, but the amount of increase was smaller than that of E. coli. In this strain, ApnA was mainly synthesized by lysyl-tRNA synthetase and was mainly degraded by ApaH. The intracellular ApnA concentration of apaH deletion mutant was a 5-10-fold higher than that of wild-type strain, and the mutant reduced spore formation. Since Ap5A strongly inhibited adenylate kinase activity, energy homeostasis can not be maintained. Therefore, ApnA is sequentially degraded by the degrading enzyme in this bacterium and is maintained at a low concentration.
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