Elucidation of Activation Mechanism of Pathogen Effector Proteins by Yeast Genetic System and Structural Analysis
| Project/Area Number |
16K07668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
櫻庭 春彦 香川大学, 農学部, 教授 (90205823)
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| Research Collaborator |
Ohnishi Kouhei
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | エフェクター / 青枯病菌 / 出芽酵母 / Ⅲ型分泌装置 / グルタチオン / チオレドキシン / 酵母 / 青枯病 / 病原因子 / 結晶構造解析 |
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| Outline of Final Research Achievements |
Many bacterial pathogens express their virulence by injecting the effector proteins into host cells. In this study, we investigated the host factor-dependent activation mechanism of the effector RipAY and RipAA derived from the phytopathogenic bacterium Ralstonia solanacearum. As a result, it was revealed that RipAY formed a complex with thioredoxin, which is a host activating factor, at a molecular ratio of 1: 2. In addition, we found that RipAA is a multi-domain protein consisting of a membrane localization domain, an autoinhibitory domain, and a growth inhibitory domain. It also clarified that RipAA was activated by phosphorylation via the plasma membrane targeting.
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| Academic Significance and Societal Importance of the Research Achievements |
ヒト及び植物に感染する病原菌は、エフェクターを宿主細胞内に注入することにより宿主内の免疫機能を阻害することで感染を成立させている。エフェクターの多くは、宿主細胞に注入されることでその活性を宿主内因子により活性化されることが知られており、これはエフェクターの活性により病原菌自身の増殖が阻害されないためにも有効であり、また、宿主内においてその活性を時間的空間的に制御されることで効率的に感染を引き起こしていることが考えられる。本研究によってこれらのエフェクターの宿主依存的な活性化メカニズムの一旦を明らかにすることができ、病原菌の感染戦略の理解に繋がる成果であると言える。
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Report
(3 results)
Research Products
(36 results)
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[Presentation] Functional analysis of Ralstonia solanacearum effector, RipAA using yeast expression system2018
Author(s)
Hirata, A., Kitasono, T., Shirai, S., Fujiwara, S., Tanaka, N., Tabuchi, M.
Organizer
Phytogene Symposium X
Related Report
Int'l Joint Research
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[Presentation] Ralstonia solanacearum effector, RipAY exhibits robust γ-glutamy cyclotransferase activity when stimulated by eukaryotic thgioredoxins2016
Author(s)
Tabuchi, M., Fujiwara, S., Ohnishi, O., Popa, C., Valls, M., Genin, S., and Tanaka, N.
Organizer
The Sixth International Bacterial Wilt Sympodium
Place of Presentation
Toulouse, France
Year and Date
2016-07-03
Related Report
Int'l Joint Research
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[Presentation] A Ralstonia solanacearum effector, RipAA causes growth inhibition that is stimulated through membrane localization and subsequent phosphorylation in yeast2016
Author(s)
Kitasono, T., Shin, S., Fujiwara, S., Tanaka, N., and Tabuchi, M.
Organizer
Phytogene Symposium VIII
Place of Presentation
Kagawa International Conference Hall.
Related Report
Int'l Joint Research
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