Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
Coiled-coil proteins Gmp1, 3 and 4 were isolated from analysis of the Golgi fraction. As a result of localization analysis, aggregates were observed in several places in the cell. This aggregate was localized to Vps35, one of the retromer-constituting proteins responsible for retrograde transport to the Golgi apparatus. Furthermore, enlargement of the endosomal fraction was observed due to overexpression of Gmp1. According to the yeast two-hybrid method, it was inferred that Gmp1 and Gmp3, and Gmp3 and Gmp4 interact to form a complex. As a result of examining the diffusion conditions of the aggregates, it was observed that they were diffused into the cytoplasm under the addition of a microtubule synthesis inhibitor or under nitrogen starvation conditions.
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