• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Control of meta-fermentation process for valuable production and development of analytical method

Research Project

Project/Area Number 16K07670
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionKyushu University

Principal Investigator

Tashiro Yukihiro  九州大学, 農学研究院, 准教授 (90448481)

Co-Investigator(Kenkyū-buntansha) 酒井 謙二  九州大学, 農学研究院, 教授 (50205704)
Research Collaborator KOHARA Toshiya  
MIZOGUCHI Takaharu  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsメタ発酵 / 有価物 / バイオマス / L-乳酸 / 食品廃棄物 / 微生物分離 / 微生物選抜 / 微生物群集構造 / 複合微生物種菌 / pH制御切替法 / 体系的フィードバック分離法 / 複合微生物 / 発酵制御 / 機能解析
Outline of Final Research Achievements

In this study, the parameters of sterilization, agitation, and pH control affected L-lactic acid production in mete-fermentation (the fermentative production of pure chemicals and fuels by a controlled mixed culture) . In particular, a novel pH control by switching from swing to constant was newly proposed, and would be the most efficient method for L-lactic acid from food waste. Furthermore, systematic feed-back isolation method was established, and we succeded in isolation of 3 major species in meta-fermentation, and it would be efficient method to the targeted bacteria using solid medium. Finally, lactic acid-producing Bacillus coagulans would be predominant by controlling fermentation temperature even using different composts and autothermal thermophilic aerobic digestion sludge, to produce L-lactic acid from food waste.

Academic Significance and Societal Importance of the Research Achievements

発展途上の学問分野である複合微生物工学領域において,本研究で確立したpH切替制御法は重要な技術として,広く応用されうる.また,微生物学領域全般で,固体培地による微生物の分離は100年以上も重要であるが,分離率はわずか1%である.本研究で確立した不体系的フィードバック分離法は,この分離率を向上させるとともに迅速化,すなわち杯スループット化の可能性を示し,微生物学領域の発展が期待される.また,生ごみから生分解性プラスチックポリL-乳酸生産が強化されれれば,資源の循環利用だけではなく,近年問題となっている石油プラスチック(マイクロプラスチック)による環境・生態系の解決方法としても有望である.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results)

  • [Journal Article] Development of a systematic feedback isolation approach for targeted strains from mixed culture systems2017

    • Author(s)
      Pramod Poudel, Yukihiro Tashiro, Hirokuni Miyamoto, Hisashi Miyamoto, Yuki Okugawa, Kenji Sakai.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 123 Issue: 1 Pages: 63-70

    • DOI

      10.1016/j.jbiosc.2016.07.019

    • NAID

      40021077184

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Novel pH control strategy for efficient production of optically active L-lactic acid from kitchen refuse using a mixed culture system2016

    • Author(s)
      Yukihiro Tashiro, Shota Inokuchi, Pramod Poudel, Yuki Okugawa, Hirokuni Miyamoto, Hisashi Miayamoto, Kenji Sakai.
    • Journal Title

      Bioresource Technology

      Volume: 216 Pages: 52-59

    • DOI

      10.1016/j.biortech.2016.05.031

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Establish of efficient meta-fermentation process for optically pure lactic acid production from waste biomass using mixed culture system2018

    • Author(s)
      Yukihiro Tashiro, Takeshi Zendo, Vichien Kitpreechavanich, Kenji Sonomoto, Kenji Sakai
    • Organizer
      The 2nd e-ASIA Joint Research Program Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reconstruction of a complex microbial system for producing optically pure L-lactic acid2018

    • Author(s)
      Takaharu Mizoguchi, Yukihiro Tashiro, Kenji Sakai
    • Organizer
      JSBBA West 1st Student Forum
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 複合微生物系から考える発酵生産2018

    • Author(s)
      田代幸寛、酒井謙二
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 複合微生物系によるメタ乳酸発酵プロセスの再構築と機能解析2018

    • Author(s)
      溝口尊春、田代幸寛、酒井謙二
    • Organizer
      第25回日本生物工学会九州支部鹿児島大会(2018)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分離菌群による光学活性L-乳酸生産のためのメタ発酵プロセスの再構築2018

    • Author(s)
      田代幸寛、溝口尊春、古原俊哉、宮本浩邦、酒井謙二
    • Organizer
      日本農芸化学会2019年度大会(東京)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 異なる種菌による複合微生物系を用いたメタ発酵による有価物生産2018

    • Author(s)
      田代幸寛、石田勇貴、木村 知弘、奥川 友紀、宮本 浩邦、酒井 謙二
    • Organizer
      日本農芸化学会2018年度大会, 名城大学, 2018年3月15-18日
    • Related Report
      2017 Research-status Report
  • [Presentation] Establish of efficient meta-fermentation process for optically pure lactic acid production from waste biomass using mixed culture system2017

    • Author(s)
      Yukihiro Tashiro, Kenji Sakai
    • Organizer
      The 4th Satellite Seminar, New Core to Core Program A. Advanced Research Networks on “Establishment of an international Research Core for New Bio-Research Fields with Microbes from Tropical Areas
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 高光学活性L-乳酸の生産のための複合微生物系の再構築2017

    • Author(s)
      溝口尊春、古原俊哉、田代幸寛、 宮本浩邦、酒井謙二
    • Organizer
      第69回日本生物工学会大会, 早稲田大学, 2017年9月11-14日
    • Related Report
      2017 Research-status Report
  • [Presentation] Establish of efficient meta-fermentation process for optically pure lactic acid production from waste biomass using mixed culture system2016

    • Author(s)
      Yukihiro Tashiro, Kenji Sakai
    • Organizer
      THE 2nd JOINT SEMINAR, New Core to Core Program A. Advanced Research Networks on “Establishment of an international Research Core for New Bio-Research Fields with Microbes from Tropical Areas”
    • Place of Presentation
      Chonburi, Thailand
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Establishment of efficient meta-fermentation process for value-added substance conversions with controlled mixed culture systems2016

    • Author(s)
      Yukihiro Tashiro, Kenji Sakai
    • Organizer
      22nd Symposium of Young Asian Biological Engineers’ Community (YABEC 2016)
    • Place of Presentation
      Miyazaki, Japan
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 複合微生物系からの体系的フィードバック分離法の開発と応用2016

    • Author(s)
      田代幸寛、Poudel Pramod、奥川友紀、酒井謙二
    • Organizer
      2016年度日本農芸化学会西日本支部大会
    • Place of Presentation
      長崎大学
    • Related Report
      2016 Research-status Report
  • [Presentation] L-乳酸の生産のための混合培養系の再構築2016

    • Author(s)
      古原俊哉、田代幸寛、酒井謙二
    • Organizer
      第23回日本生物工学会九州支部飯塚大会(2016),
    • Place of Presentation
      九州工業大学飯塚キャンパス
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-12-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi