Control of meta-fermentation process for valuable production and development of analytical method
| Project/Area Number |
16K07670
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
酒井 謙二 九州大学, 農学研究院, 教授 (50205704)
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| Research Collaborator |
KOHARA Toshiya
MIZOGUCHI Takaharu
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | メタ発酵 / 有価物 / バイオマス / L-乳酸 / 食品廃棄物 / 微生物分離 / 微生物選抜 / 微生物群集構造 / 複合微生物種菌 / pH制御切替法 / 体系的フィードバック分離法 / 複合微生物 / 発酵制御 / 機能解析 |
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| Outline of Final Research Achievements |
In this study, the parameters of sterilization, agitation, and pH control affected L-lactic acid production in mete-fermentation (the fermentative production of pure chemicals and fuels by a controlled mixed culture) . In particular, a novel pH control by switching from swing to constant was newly proposed, and would be the most efficient method for L-lactic acid from food waste. Furthermore, systematic feed-back isolation method was established, and we succeded in isolation of 3 major species in meta-fermentation, and it would be efficient method to the targeted bacteria using solid medium. Finally, lactic acid-producing Bacillus coagulans would be predominant by controlling fermentation temperature even using different composts and autothermal thermophilic aerobic digestion sludge, to produce L-lactic acid from food waste.
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| Academic Significance and Societal Importance of the Research Achievements |
発展途上の学問分野である複合微生物工学領域において，本研究で確立したpH切替制御法は重要な技術として，広く応用されうる．また，微生物学領域全般で，固体培地による微生物の分離は100年以上も重要であるが，分離率はわずか1％である．本研究で確立した不体系的フィードバック分離法は，この分離率を向上させるとともに迅速化，すなわち杯スループット化の可能性を示し，微生物学領域の発展が期待される．また，生ごみから生分解性プラスチックポリL-乳酸生産が強化されれれば，資源の循環利用だけではなく，近年問題となっている石油プラスチック（マイクロプラスチック）による環境・生態系の解決方法としても有望である．
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Report
(4 results)
Research Products
(14 results)
