| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
Geobacter sulfurreducens has been historically studied as iron- and electrode-reducing microorganism. G. sulfurreducens can transfer electrons from inside the cell to outside via various c-type cytochromes. Among the c-type cytochromes, OmcZ is essential for electron transfer to the electrode. In this study, OmcZ-overexpressing G. sulfurreducens was generated and OmcZ was purified by affinity chromatography by FPLC. Purified OmcZ was crystallized by sitting drop method. X-ray diffraction data at 3.4 angstrom were obtained.
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