| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In order to develop the production method of bioethanol from cellulosic biomass, we searched for enzymes involved in pentose metabolism from yeasts and filamentous fungi and characterized them. A xylitol dehydrogenase with L-arabitol dehydrogenase activity was found in a yeast which had been isolated from alcoholic starter from Thailand. An L-xylulose reductase was also found in a zygomycetous fungus. These two enzymes were purified to homogeneity and characterized, and their genes were cloned and used to construct expression system in E. coli. Random mutagenesis was introduced into each genes and mutant enzymes with higher thermostability were obtained.
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