Development of gene introduction system for stable production of secondary metobolites in engineered Streptomyces host
Project/Area Number |
16K07674
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | Kitasato University |
Principal Investigator |
KOMATSU MAMORU 北里大学, 感染制御科学府, 講師 (40414057)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 放線菌 / 物質生産 / 二次代謝産物 / 接合伝達 / 線状プラスミド / 生合成遺伝子 / 異種発現 / 二次代謝産物生産 / 合成生物学 |
Outline of Final Research Achievements |
The purpose of this study was to develop a stable secondary metabolite production system in heterologous expression of exogenous gene cluster for secondary metabolite biosynthesis derived from heterogenous microorganisms using an engineered Stretomyces avermitilis as a host. In this study, we developed an efficient method to introduce a large gene cluster for secondary metabolite biosynthesis. In addition, we developed an experimental method for stable retention of the introduced gene cluster in cells, a multiple gene introduction system and a combinatorial biosynthesis by the multiple gene introduction system.
|
Academic Significance and Societal Importance of the Research Achievements |
S.avermitilis は認定宿主として登録されており、大腸菌などと同様に取り扱うことが可能である。本菌は現在も抗生物質生産のために利用されており、その様な産業微生物を二次代謝産物生産の為の包括的な異種発現宿主として利用する試みは成されていない。遺伝的な背景および遺伝子導入系が整備されていない生産菌の保有する二次代謝産物について、本研究によって得られる成果により、その生合成遺伝子群を汎用宿主に導入すれば、生産過程の包括的な解析の展開が可能となる。さらに、これまで遺伝的な不安定性あるいは分子遺伝学的な基盤の整備が乏しくて解析が断念された多くの二次代謝産物についても適用が期待できる。
|
Report
(4 results)
Research Products
(6 results)
-
-
[Journal Article] 1.Neothioviridamide, a Polythioamide Compound Produced by Heterologous Expression of a Streptomyces sp. Cryptic RiPP Biosynthetic Gene Cluster.2018
Author(s)
Kawahara T, Izumikawa M, Kozone I, Hashimoto J, Kagaya N, Koiwai H, Komatsu M, Fujie M, Sato N, Ikeda H, Shin-Ya K.
-
Journal Title
J. Nat. Prod.
Volume: 81
Issue: 2
Pages: 264-269
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Heterologous production of kasugamycin, an aminoglycoside antibiotic from Streptomyces kasugaensis, in Streptomyces lividans and Rhodococcus erythropolis L-88 by constitutive expression of the biosynthetic gene cluster2017
Author(s)
Kasuga, K., Sasaki, A., Matsuo, T., Yamamoto, C., Minato, Y., Kuwahara, N., Fujii, C., Kobayashi, M., Agematsu, H., Tamura, T., Komatsu, M., Ishikawa, J., Ikeda, H., Kojima, I.
-
Journal Title
Appl. Microbiol. Biotechnol
Volume: -
Issue: 10
Pages: 4259-4268
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-