Study on diverse photo-sensing switches in non-phototrophic bacteria
| Project/Area Number |
16K07676
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
TAKANO Hideaki 日本大学, 生物資源科学部, 准教授 (50385994)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 光センサー / LitR/CarHファミリー / ビタミンB12 / カロテノイド / Burkholderia属細菌 / LitRファミリー / 微生物スクリーニング / ゲノムマイニング / 非光合成細菌 / バクテリア / LitR / LOV |
|---|
| Outline of Final Research Achievements |
We screened conditions for crystallization of class II LitR-LOV protein complex, but we were unable to identify the proper condition among 5,800 conditions. We showed that class III LitR serves as UV-A sensor, and controls the expression of folate biosynthesis gene. We also found thatγ-PGA production in Bacillus subtilis natto is repressed in a photo-dependent manner.
|
| Academic Significance and Societal Importance of the Research Achievements |
グラム陰性細菌Burkholderia multivoransのクラスIII LitRが、一次代謝の葉酸合成遺伝子の光依存的な発現に関与することを示した最初の例である。また、クラスIII LitRは転写調節とUV-Aセンサーの２機能を合わせもったバクテリアからの初めての例と予想されることから、学術的に高い意義を有する。納豆菌において見出したユニークな現象およびその研究成果は、光によるγ-ポリグルタミンの精密な生産制御技術の基盤となることが見込まれる。
|
Report
(4 results)
Research Products
(30 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] High production of a class III lantipeptide AmfS in Streptomyces griseus2017
Author(s)
Hideaki Takano, Yuhei Matsui, Junpei Nomura, Masahiro Fujimoto, Naoto Katsumata, Takafumi Koyama, Isamu Mizuno, Shoichi Amano, Hatsumi Shiratori-Takano, Mamoru Komatsu, Haruo Ikeda, and Kenji Ueda
Organizer
XVIII. International symposium on the Biology of Actinomycetes
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
