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Regulation of insertion sequnces found in the genome of Bacillus subtilis (natto)

Research Project

Project/Area Number 16K07683
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

KIMURA Keitarou  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, ユニット長 (20353980)

Research Collaborator YOSHIKAWA Hirofumi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords挿入配列 / ゲノム進化 / 納豆菌 / 転移 / 遺伝子破壊 / insertion sequnce / transposition / IS256Bsu1 / insertion sequence / 発現制御 / Bacillus subtilis / transposase / trasposition frequency / トランスポゾン / RNA-seq / Insertion sequence / 微生物 / 発酵
Outline of Final Research Achievements

Insertion sequence (IS) is a mobile genetic element consist of a transposase gene and an inverted repeat sequence. ISs translocate in the genome and can disrupt gene(s), thus have committed on structural evolution of genome. This study started to elucidate regulatory mechanism of ISs found in the genome of Bacillus subtilis (natto).
On of the most important findings obtained in this study is suppressive function of the gene BSNT_10618. Disruption of BSNT_10618 greatly increased the frequency of transposition of the insertion sequence which suggested that Bacillus subtilis (natto) can harbor many copies of IS by suppressing the translocation of IS with the BSNT_10618.

Academic Significance and Societal Importance of the Research Achievements

挿入配列(以下、IS)の発現制御に関する先行研究として,大腸菌で見つかったIS転移促進タンパク質Ieeが挙げられる.本研究では,Ieeと部分的な類似性を持つ納豆菌BSNT_10618が,むしろ転移を抑制する機能を持つことが明らかになった.納豆菌のように非常に多種多コピーのISを有する株が転移を抑制する仕組みを持つ可能性が強く示唆されたことに学術的意義がある.また,RNS-seq解析(DNA配列解析装置による網羅的な遺伝子発現解析)を通じて,東京農大が開発したIS転移頻度測定法(Jumping CAT法)の再現性と妥当性が確認できたことは科学的に意義深く,継続的な研究深化に繋がる成果である.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2016 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (1 results) (of which Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] USDA/ARS/南部研究所(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Trends in the application of Bacillus in fermented foods2019

    • Author(s)
      Kimura (木村 啓太郎) Keitarou、Yokoyama (横山 智) Satoshi
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 56 Pages: 36-42

    • DOI

      10.1016/j.copbio.2018.09.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Poly-γ-glutamic Acid Production by <i>Bacillus subtilis</i> (natto) under High Salt Conditions2018

    • Author(s)
      NGUYEN Sy Le Thanh、INAOKA Takashi、KIMURA Keitarou
    • Journal Title

      Japan Agricultural Research Quarterly: JARQ

      Volume: 52 Issue: 3 Pages: 249-253

    • DOI

      10.6090/jarq.52.249

    • NAID

      130007437004

    • ISSN
      0021-3551, 2185-8896
    • Year and Date
      2018-07-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolome analysis of Escherichia coli ATCC25922 cells treated with high hydrostatic pressure at 400 and 600 MPa2018

    • Author(s)
      Kimura Keitarou、Inaoka Takashi、Yamamoto Kazutaka
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 126 Issue: 5 Pages: 611-616

    • DOI

      10.1016/j.jbiosc.2018.05.007

    • NAID

      40021726353

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Survey of Phage Contamination in Natto-producing Factories and Development of Phage-resistant <i>Bacillus subtilis</i> (natto) Strains2018

    • Author(s)
      Kubo Yuji、Sriyam Supawadee、Nakagawa Rikio、Kimura Keitarou
    • Journal Title

      Food Science and Technology Research

      Volume: 24 Issue: 3 Pages: 485-492

    • DOI

      10.3136/fstr.24.485

    • NAID

      130007419039

    • ISSN
      1344-6606, 1881-3984
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 納豆菌に関する基礎研究と応用利用研究2016

    • Author(s)
      木村啓太郎
    • Organizer
      日本乳酸菌学会(泊り込みセミナー)
    • Place of Presentation
      華やぎの章 慶山 (山梨県笛吹市)
    • Year and Date
      2016-05-12
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] なっとう菌2018

    • Author(s)
      木村啓太郎、高部晴市
    • Total Pages
      32
    • Publisher
      農山漁村文化協会
    • ISBN
      9784540171772
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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