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Elucidation of structure and function of two basidiomycete proteins belonging to glycoside hydrolase family 131

Research Project

Project/Area Number 16K07687
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Tonozuka Takashi  東京農工大学, (連合)農学研究科(研究院), 教授 (50285194)

Research Collaborator YOSHIDA Makoto  
IGARASHI Kiyohiko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsセルロース / バイオマス / キシラン / 担子菌 / 糖質加水分解酵素 / バイオマス分解 / GH131
Outline of Final Research Achievements

This study focuses on the analysis of the function of two enzymes belonging to glycoside hydrolase family (GH) 131 from a basidiomycete Coprinopsis cinerea, CcGH131A and CcGH131B. A catalytic residue of CcGH131B was modified and the resulted variant was crystallized. The crystals were then soaked in various solutions containing sugars, and the structures were determined using the X-ray crystallographic analysis. The results indicated that cellobiose was found in the structure of CcGH131B. The analysis of CcGH131A was also carried out, indicating that CcGH131A acted on xylan. These findings suggest that the GH131 enzymes widely acted on cellulosic biomass with divergent functions.

Academic Significance and Societal Importance of the Research Achievements

近年、化石燃料の使用に伴う地球温暖化により、気候変動が自然や社会に対して深刻な影響を起こすことが懸念されており、再生可能エネルギーに関する技術の開発が嘱望されている。本研究はセルロース系バイオマスの効率的な利用を目標とし、セルロース製剤によって発現が誘導される機能未知酵素がバイオマス分解に果たす役割の一端を示した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2019 2018 2017 2016

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] 澱粉の酵素分解-研究の歴史的背景から最近の話題まで-2018

    • Author(s)
      殿塚隆史
    • Journal Title

      応用糖質科学

      Volume: 8 Pages: 267-275

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Mutagenesis-induced conformational change in domain B of a pullulan-hydrolyzing α-amylase TVA I2018

    • Author(s)
      Tonozuka, T., Nihira, T., Mizuno, M., Nishikawa, A. and Kamitori, S.
    • Journal Title

      Amylase

      Volume: 2 Issue: 1 Pages: 1-10

    • DOI

      10.1515/amylase-2018-0001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure of a β-fructofuranosidase with high transfructosylation activity from Aspergillus kawachii2017

    • Author(s)
      Nagaya, M., Kimura, M., Gozu, Y., Sato, S., Hirano, K., Tochio, T., Nishikawa, A. and Tonozuka, T.
    • Journal Title

      Bioscience Biotechnology and Biochemistry

      Volume: 81 Issue: 9 Pages: 1786-1795

    • DOI

      10.1080/09168451.2017.1353405

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure of the catalytic domain of α-L-arabinofuranosidase from Coprinopsis cinerea, CcAbf62A, provides insights into structure-function relationships in glycoside hydrolase family 622017

    • Author(s)
      Tonozuka, T., Tanaka, Y., Okuyama, S., Miyazaki, T., Nishikawa, A. and Yoshida, M.
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 181 Issue: 2 Pages: 511-525

    • DOI

      10.1007/s12010-016-2227-0

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase2016

    • Author(s)
      Miyazaki, T., Nishikawa, A. and Tonozuka, T.
    • Journal Title

      Journal of Structural Biology

      Volume: 196 Issue: 3 Pages: 479-486

    • DOI

      10.1016/j.jsb.2016.09.015

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Aspergillus niger 由来の糖転移活性を有する新規α-グルコシダーゼの結晶化2019

    • Author(s)
      堤賢太、 石川涼一、 松本雄治、 安武望、 西河淳、 殿塚隆史
    • Organizer
      量子ビームサイエンスフェスタ
    • Related Report
      2018 Annual Research Report
  • [Presentation] Expression, purification, and some properties of a GH13 enzyme from Rhodothermus marinus2019

    • Author(s)
      Qu Pu、長屋美香、藤井義晴、西河淳、殿塚隆史
    • Organizer
      日本農芸化学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 担子菌のGH131に属する酵素の立体構造と機能の解析2018

    • Author(s)
      殿塚隆史
    • Organizer
      セルラーゼ研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] プルラン分解α-アミラーゼTVA Iの改変酵素におけるドメインBのコンフォメーション変化2018

    • Author(s)
      殿塚隆史、西河淳、神鳥成弘
    • Organizer
      日本応用糖質科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Flavobacterium johnsoniae 由来GH31デキストラナーゼFjDex31A改変酵素の性質解析2018

    • Author(s)
      堤賢太、郷津佳史、西河淳、殿塚隆史
    • Organizer
      日本応用糖質科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Studies of GH63 and GH31 enzymes ‐ Exploration of the properties of uncharacterized glycoside hydrolases by structural analysis2018

    • Author(s)
      Takashi Tonozuka
    • Organizer
      Danish‐Japanese Network for Glycobiology
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 担子菌Coprinopsis cinerea由来CcGH131Bと各種リガンドとのX線結晶構造解析2018

    • Author(s)
      石川涼一、吉田誠、砂川直輝、五十嵐圭日子、西河淳、殿塚隆史
    • Organizer
      日本結晶学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 担子菌Coprinopsis cinerea由来CcGH131Bとセロビオースの複合体の立体構造解析2018

    • Author(s)
      石川涼一、奥山舜朔、砂川直輝、五十嵐圭日子、西河淳、吉田誠、殿塚隆史
    • Organizer
      量子ビームサイエンスフェスタ
    • Related Report
      2017 Research-status Report
  • [Presentation] Aspergillus kawachii由来β-フルクトフラノシダーゼの改変による1-ケストース生産能向上と改変酵素の構造解析2018

    • Author(s)
      長屋美香、平野勝紹、藤井匡、栃尾巧、西河淳、殿塚隆史
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 担子菌Coprinopsis cinerea由来α-L-アラビノフラノシダーゼCcAbf62Aの触媒ドメインのX線結晶構造解析2017

    • Author(s)
      殿塚隆史、田中祐太郎、奥山舜朔、宮崎剛亜、西河淳、吉田誠
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] GH31に属するFlavobacterium johnsoniae由来デキストラナーゼの結晶化と構造解析2017

    • Author(s)
      郷津佳史、石川涼一、西河淳、殿塚隆史
    • Organizer
      2016年度量子ビームサイエンスフェスタ
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 担子菌Coprinopsis cinerea由来CcGH131Bとキシロオリゴ糖との複合体の立体構造解析2017

    • Author(s)
      石川涼一、奥山舜朔、砂川直輝、五十嵐圭日子、西河淳、吉田誠、殿塚隆史
    • Organizer
      日本応用糖質科学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Flavobacterium johnsoniae 由来GH31デキストラナーゼFjDex31Aとグルコースとの複合体の立体構造解析2017

    • Author(s)
      郷津佳史、石川涼一、西河淳、殿塚隆史
    • Organizer
      日本応用糖質科学会
    • Related Report
      2017 Research-status Report
  • [Presentation] GH31に属するFlavobacterium johnsoniae由来デキストラナーゼ(FjDex31A)の性質解析2016

    • Author(s)
      郷津佳史、石嵜雄一、石川涼一、西河淳、殿塚隆史
    • Organizer
      日本生化学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Research-status Report
  • [Presentation] Arthrobacter globiformis GH27 isomalto-dextranase and Flavobacterium johnsoniae GH31 dextranase/glucosyltransferase - Two dextran-hydrolyzing enzymes belonging to clan GH-D2016

    • Author(s)
      Gozu, Y., Ishizaki, Y., Miyazaki, T., Ishikawa, R., Okazawa, Y., Nishikawa, A. and Tonozuka, T.
    • Organizer
      6th Symposium on the Alpha-Amylase Family
    • Place of Presentation
      Smolenice Castle (Smolenice, Slovakia)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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