Molecular mechanisms of bipolar spindle assembly in the absence of Kinesin-5/Cut7
| Project/Area Number |
16K07694
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Yukawa Masashi 広島大学, 先端物質科学研究科, 特任助教 (50403605)
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| Research Collaborator |
TODA takashi
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 染色体分配 / 紡錘体 / 微小管 / キネシンモーター / 5型キネシン / 14型キネシン / 微小管ポリメラーゼ / 有糸分裂 / ５型キネシン / １４型キネシン / ６型キネシン / キネシン / 分裂酵母 |
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| Outline of Final Research Achievements |
The assembly of a bipolar spindle is necessary to ensure the fidelity of chromosome segregation. In all eukaryotes, spindle formation is driven by the plus-end directed motor kinesin-5. Inactivation of this kinesin causes mitotic arrest with monopolar spindles. Interestingly, simultaneous inactivation of the minus-end directed kinesin-14 restores cell viability in fission yeast. Apparent normal spindle assembly in the absence of both kinesin-5 and kinesin-14 has highlighted the existence of other hitherto unknown factors by which to establish and maintain mitotic bipolar spindles. To uncover these molecules, we have conducted two complementary independent genetic screens. Consequently, we have succeeded in identifying kinesins, microtubule-associated proteins and their regulators.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果により同定に成功した新規紡錘体形成因子はいずれも酵母からヒトまで進化上高度に保存されていることから、高等生物においても類似した紡錘体形成機構が存在すると考えられる。また、これらの新規紡錘体形成因子を阻害することにより、がん細胞の増殖を抑えることができると考えられ、副作用の少ないがん治療薬の開発に繋がることが期待される。
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation2016
Author(s)
Matsuo, Y., Maurer, S. P., Yukawa, M., Zakian, S., Singleton, M. R., Surrey, T. and Toda,
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Journal Title
J Cell Sci
Volume: 129
Issue: 24
Pages: 4592-4606
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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